##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_17_TGACCA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	33021066	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5049145294098	34.0	31.0	34.0	31.0	34.0
2	32.840345553956375	34.0	31.0	34.0	31.0	34.0
3	32.97184027917209	34.0	31.0	34.0	31.0	34.0
4	36.44204720707684	37.0	37.0	37.0	35.0	37.0
5	36.36537048803936	37.0	37.0	37.0	35.0	37.0
6	36.34697477664712	37.0	37.0	37.0	35.0	37.0
7	36.33472835189512	37.0	37.0	37.0	35.0	37.0
8	36.3339241380033	37.0	37.0	37.0	35.0	37.0
9	38.10606193028414	39.0	38.0	39.0	37.0	39.0
10	38.103027867119735	39.0	38.0	39.0	37.0	39.0
11	38.08185508002679	39.0	38.0	39.0	37.0	39.0
12	38.07368217609934	39.0	38.0	39.0	37.0	39.0
13	38.06024826696994	39.0	38.0	39.0	37.0	39.0
14	39.598268026840806	41.0	40.0	41.0	37.0	41.0
15	39.59622599706503	41.0	40.0	41.0	37.0	41.0
16	39.585583942081094	41.0	40.0	41.0	37.0	41.0
17	39.53890852584832	41.0	40.0	41.0	37.0	41.0
18	39.52084866067013	41.0	39.0	41.0	37.0	41.0
19	39.528534360459474	41.0	40.0	41.0	37.0	41.0
20	39.50491719437525	41.0	39.0	41.0	37.0	41.0
21	39.49069497029563	41.0	39.0	41.0	37.0	41.0
22	39.46100153156776	41.0	39.0	41.0	37.0	41.0
23	39.43019704451697	41.0	39.0	41.0	37.0	41.0
24	39.40666470307167	41.0	39.0	41.0	36.0	41.0
25	39.363064384414486	41.0	39.0	41.0	36.0	41.0
26	39.298940076616546	41.0	39.0	41.0	36.0	41.0
27	39.23613780366751	40.0	39.0	41.0	36.0	41.0
28	39.169054112305155	40.0	39.0	41.0	36.0	41.0
29	39.10762084422108	40.0	39.0	41.0	36.0	41.0
30	39.049663902431256	40.0	39.0	41.0	36.0	41.0
31	38.98493570740569	40.0	39.0	41.0	36.0	41.0
32	32.44768451751376	40.0	35.0	41.0	2.0	41.0
33	38.84954186518388	40.0	38.0	41.0	35.0	41.0
34	38.96691596812774	40.0	39.0	41.0	36.0	41.0
35	38.99466916058979	40.0	39.0	41.0	35.0	41.0
36	38.98510647718035	40.0	39.0	41.0	35.0	41.0
37	38.947527072566345	40.0	39.0	41.0	35.0	41.0
38	38.900087295788694	40.0	39.0	41.0	35.0	41.0
39	38.77192517043514	40.0	38.0	41.0	35.0	41.0
40	38.66161961579314	40.0	38.0	41.0	35.0	41.0
41	38.68603269803585	40.0	38.0	41.0	35.0	41.0
42	38.68584512080864	40.0	38.0	41.0	35.0	41.0
43	38.639483685959746	40.0	38.0	41.0	35.0	41.0
44	38.56515843552719	40.0	38.0	41.0	35.0	41.0
45	38.54666723963424	40.0	38.0	41.0	35.0	41.0
46	38.46722243915445	40.0	38.0	41.0	34.0	41.0
47	38.40695906061906	40.0	38.0	41.0	34.0	41.0
48	38.344580820013505	40.0	38.0	41.0	34.0	41.0
49	38.30369010497723	40.0	38.0	41.0	34.0	41.0
50	38.12693224379855	40.0	38.0	41.0	34.0	41.0
51	37.971103991615536	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	4.0
11	21.0
12	58.0
13	94.0
14	192.0
15	305.0
16	518.0
17	812.0
18	1522.0
19	2625.0
20	4932.0
21	8786.0
22	15084.0
23	23860.0
24	36246.0
25	51449.0
26	71299.0
27	97171.0
28	129264.0
29	171421.0
30	223763.0
31	290806.0
32	380631.0
33	514166.0
34	736617.0
35	1095024.0
36	1719234.0
37	3127589.0
38	8270098.0
39	1.6040086E7
40	7383.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.842080683005683	29.65159480381449	28.43430923791454	23.072015275265287
2	20.917295643938328	28.978589001336296	30.78952387545575	19.314591479269627
3	19.90058743458269	27.811995614910444	31.63859438484909	20.648822565657778
4	20.488421095743227	27.70434699579673	31.337522767423277	20.469709141036763
5	23.814864123405343	27.838840817555678	28.57400484890464	19.772290210134344
6	22.069621493858318	27.522115717071816	29.803260826805243	20.60500196226462
7	23.649106300808096	28.135563521783336	27.92704814556865	20.288282031839916
8	21.170909503648367	27.747153892609038	30.23192225229797	20.85001435144462
9	21.016014443628197	27.565430504272637	30.65168762268305	20.76686742941612
10	21.241337272394535	27.8426171947326	30.30766178172443	20.608383751148434
11	23.247262074513728	27.737276654675775	28.345176591973054	20.670284678837444
12	21.167968956544286	29.771522215545676	28.280313542875934	20.780195285034107
13	22.433021998744678	27.942047661332314	28.667645072391064	20.957285267531944
14	22.101353245310325	27.977023998984407	28.828650100432863	21.092972655272405
15	22.165226283124838	27.975226481180226	28.63608643040173	21.223460805293204
16	22.117290217099594	27.930412664448813	28.642124999841013	21.310172118610588
17	22.187616037934145	27.97566542549615	28.543302555454396	21.29341598111531
18	22.074941165322574	27.933091621895294	28.590535508144733	21.401431704637396
19	21.903310449442166	28.092260556866083	28.734248113589377	21.27018088010237
20	21.786848499423375	28.104018352381853	28.723300097487414	21.385833050707355
21	21.880726079527534	28.075913721259028	28.61434879176826	21.429011407445174
22	21.870147983714396	28.07959016223159	28.663826298036533	21.38643555601748
23	21.937956212558372	28.10478922757975	28.556885474260586	21.400369085601294
24	21.850845154423542	28.11679974232207	28.516444623562425	21.515910479691964
25	21.88878457164284	28.21375300240156	28.44451175501118	21.45295067094442
26	21.918807830128802	28.169832554769737	28.42116908036827	21.49019053473319
27	21.945996534454704	28.11612138748034	28.335766022817072	21.602116055247883
28	21.9431892356231	28.15612009618345	28.3775242143909	21.52316645380255
29	21.94424491364022	28.206008621123818	28.327863149100523	21.521883316135444
30	21.953652253382735	28.123846758914446	28.295167696887795	21.62733329081502
31	22.04479710012996	28.11980994193222	28.22013983437119	21.615253123566635
32	22.05160911274275	28.18194376923045	28.15010849464515	21.616338623381655
33	22.067982967841893	28.11634330994473	28.117569799762666	21.698103922450716
34	22.053623192970377	28.172291093941432	28.11548090991266	21.658604803175532
35	22.053064307380833	28.14752499723433	28.08673026487152	21.712680430513316
36	22.093492829646316	28.18926784093836	28.02658366099064	21.69065566842469
37	22.089374704014702	28.13485791161315	28.06570811493487	21.710059269437274
38	22.059957119494566	28.149805945089717	28.121184821834643	21.66905211358107
39	22.09087677544995	28.162615949466925	28.046705094257103	21.699802180826023
40	22.12880172096206	28.1792849503794	28.008066971795127	21.683846356863416
41	22.06335815103761	28.083633583327934	28.1014607689833	21.751547496651156
42	22.067425953594817	28.042879574213337	28.063563942742277	21.826130529449575
43	22.087872632579458	28.114688968551167	27.99622822594522	21.801210172924158
44	22.14494831874901	28.07015981858369	27.9752234528104	21.809668409856908
45	22.125523390661446	28.015702484519693	28.02179869618598	21.83697542863288
46	22.049713781909254	28.10050922519417	28.017440320856934	21.83233667203964
47	22.10572501196326	28.11521705042536	27.987859340363507	21.79119859724787
48	22.137791062740558	28.057000299632428	27.95656559336639	21.848643044260623
49	22.137501210974335	28.10668481757828	27.93803118391786	21.81778278752952
50	22.071143872686857	28.227763303087478	27.903335985756094	21.797756838469574
51	22.137059546893877	28.030419076250645	27.941542165073447	21.890979211782028
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.91409595827097
2	40.231887123207954
3	40.549410000240464
4	40.958130236779986
5	43.58715433353969
6	42.67462345612294
7	43.93738833264801
8	42.02092385509299
9	41.78288187304432
10	41.84972102354297
11	43.917546753351175
12	41.94816424157839
13	43.39030726627662
14	43.19432590058273
15	43.388687088418045
16	43.42746233571018
17	43.48103201904945
18	43.476372869959974
19	43.17349132954454
20	43.17268155013073
21	43.30973748697271
22	43.25658353973188
23	43.33832529815966
24	43.366755634115506
25	43.34173524258726
26	43.40899836486199
27	43.54811258970258
28	43.466355689425654
29	43.466128229775656
30	43.58098554419775
31	43.660050223696594
32	43.667947736124404
33	43.766086890292605
34	43.712227996145906
35	43.765744737894146
36	43.784148498071005
37	43.799433973451976
38	43.72900923307564
39	43.790678956275976
40	43.81264807782547
41	43.81490564768877
42	43.893556483044385
43	43.88908280550361
44	43.95461672860591
45	43.962498819294325
46	43.88205045394889
47	43.89692360921113
48	43.98643410700118
49	43.95528399850386
50	43.868900711156435
51	44.028038758675905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10774.0
1	6949.5
2	3125.0
3	4323.0
4	5521.0
5	7041.5
6	8562.0
7	12697.5
8	16833.0
9	16340.0
10	15847.0
11	20424.5
12	25002.0
13	34505.0
14	44008.0
15	67252.0
16	90496.0
17	141621.5
18	192747.0
19	241005.5
20	289264.0
21	339988.5
22	390713.0
23	463245.0
24	535777.0
25	641585.5
26	879818.0
27	1012242.0
28	1084623.0
29	1157004.0
30	1222424.0
31	1287844.0
32	1381718.5
33	1475593.0
34	1570840.0
35	1666087.0
36	1826427.5
37	1986768.0
38	2053915.5
39	2121063.0
40	2193502.0
41	2265941.0
42	2269239.0
43	2272537.0
44	2270469.0
45	2268401.0
46	2221160.0
47	2173919.0
48	2114184.0
49	2054449.0
50	1952709.0
51	1850969.0
52	1728474.0
53	1605979.0
54	1475831.0
55	1345683.0
56	1216054.0
57	1086425.0
58	956552.0
59	826679.0
60	724450.0
61	622221.0
62	530601.0
63	438981.0
64	372601.5
65	306222.0
66	256922.5
67	207623.0
68	188069.0
69	168515.0
70	146051.0
71	123587.0
72	103960.0
73	84333.0
74	69924.5
75	46456.0
76	37396.0
77	35319.0
78	33242.0
79	32527.5
80	31813.0
81	27813.5
82	23814.0
83	20228.5
84	16643.0
85	15533.0
86	14423.0
87	15928.0
88	17433.0
89	11030.5
90	4628.0
91	2666.5
92	705.0
93	474.0
94	243.0
95	151.5
96	60.0
97	39.0
98	18.0
99	11.0
100	4.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.02330330583512961
2	0.0
3	0.0057539026753406445
4	0.030538081356913188
5	0.0
6	0.0016504615568740271
7	0.0
8	0.0
9	0.0
10	0.0
11	5.17851240780658E-4
12	0.0
13	0.0
14	6.05673965825331E-6
15	0.0
16	0.0
17	0.011647110362821116
18	4.512271045398716E-4
19	6.359576641165975E-5
20	0.0011901493428467754
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0016504615568740271
30	0.0
31	0.0
32	17.48750328048162
33	3.028369829126655E-6
34	0.0017503977612352065
35	3.4220579069131206E-4
36	9.38794647029263E-5
37	0.0
38	0.0
39	0.0
40	3.028369829126655E-6
41	0.02933279016492078
42	0.002913291775619842
43	0.0
44	0.0
45	0.0016928587344818002
46	0.004512271045398716
47	0.016344111967796556
48	0.0018412488561090061
49	4.088299269320984E-4
50	0.018070282770398752
51	0.10257391448234893
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.3021066E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.9320535089896
#Duplication Level	Relative count
1	100.0
2	10.47109274736282
3	2.91169749898269
4	1.328450245719473
5	0.8288728206091339
6	0.5703195918239584
7	0.4031677465802736
8	0.3255391742573638
9	0.2911071462109118
10++	8.077127742824052
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	4711495	2.7525601	21.658485	4
TGTTT	4730585	2.1165354	16.723188	6
GAGGG	1931585	1.9576579	6.941232	11
CTTTG	3197830	1.9159286	21.51634	1
GTTTG	3235765	1.8904061	20.860064	7
TTTGT	3923000	1.7552097	16.363066	2
TTTGA	3811360	1.7350051	16.54281	8
TGAGG	2162145	1.6781871	8.3422365	10
GTGTT	2821485	1.6483743	20.773304	5
TTGTG	2707005	1.5814927	20.772766	3
TTGAG	2483975	1.4765066	13.2313175	9
>>END_MODULE