FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_12_CCGTCC_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_12_CCGTCC_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 37435085
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 14309870 3.7675948 4.70764 17
TGTGT 7258025 3.3687313 34.099182 4
AAAAA 9059290 3.0273597 3.2164752 44
GTTTG 6022735 2.7953846 33.782124 7
TGTTT 7568410 2.6457136 26.029331 6
GGGGG 2370555 2.5752945 5.468358 13
GTGTT 5389855 2.5016403 33.631874 5
CTTTG 4897210 2.4237375 35.98837 1
TTTGA 6555850 2.4036734 26.878368 8
GAGGG 2655540 2.278916 11.030954 11
TTGTG 4890525 2.2698822 33.558655 3
TTTGT 5781020 2.0208898 25.618006 2
TTGAG 4097515 1.9946958 21.325865 9
TGAGG 2956890 1.9111772 13.088755 10
AGGGG 1959405 1.6815108 5.933628 12
AACTT 3881280 1.5915442 11.109361 2
TGAGC 2148265 1.4806166 5.957329 10
ACTTT 3709900 1.4504329 10.097102 3
TGAGT 2437925 1.186797 6.2186136 10
TTGAT 3026010 1.1094731 6.195456 9
TAACT 2663130 1.0920337 10.769517 1
TTGAC 1971765 1.0235293 5.6665893 9