##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_12_CCGTCC_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	37435085	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.087539697051575	33.0	31.0	34.0	30.0	34.0
2	32.25864274116113	34.0	31.0	34.0	30.0	34.0
3	32.31989955412149	34.0	31.0	34.0	30.0	34.0
4	35.78952301029903	37.0	35.0	37.0	35.0	37.0
5	35.810523737290836	37.0	35.0	37.0	35.0	37.0
6	35.803234051692414	37.0	36.0	37.0	35.0	37.0
7	35.84473407767072	37.0	36.0	37.0	35.0	37.0
8	35.8471058099641	37.0	36.0	37.0	35.0	37.0
9	37.571219512390584	39.0	38.0	39.0	35.0	39.0
10	37.56307095335833	39.0	38.0	39.0	35.0	39.0
11	37.52331300970734	39.0	38.0	39.0	35.0	39.0
12	37.497603625048534	39.0	38.0	39.0	35.0	39.0
13	37.492243573107956	39.0	38.0	39.0	35.0	39.0
14	38.934069817124765	41.0	39.0	41.0	36.0	41.0
15	38.89509870219341	41.0	39.0	41.0	36.0	41.0
16	38.84737496923007	41.0	39.0	41.0	36.0	41.0
17	38.839210836572164	41.0	39.0	41.0	36.0	41.0
18	38.77758648070387	41.0	39.0	41.0	35.0	41.0
19	38.79516365463041	41.0	39.0	41.0	35.0	41.0
20	38.78434001151594	40.0	39.0	41.0	35.0	41.0
21	38.74671049898778	40.0	39.0	41.0	35.0	41.0
22	38.67497450586796	40.0	39.0	41.0	35.0	41.0
23	38.64975362016675	40.0	39.0	41.0	35.0	41.0
24	38.60373064466128	40.0	39.0	41.0	34.0	41.0
25	38.56361490831395	40.0	39.0	41.0	34.0	41.0
26	38.503093501724386	40.0	38.0	41.0	34.0	41.0
27	38.45832437671773	40.0	38.0	41.0	34.0	41.0
28	38.39059278214541	40.0	38.0	41.0	34.0	41.0
29	38.32611882676372	40.0	38.0	41.0	34.0	41.0
30	38.289268983895724	40.0	38.0	41.0	34.0	41.0
31	38.23765382127488	40.0	38.0	41.0	34.0	41.0
32	38.174147273874226	40.0	38.0	41.0	34.0	41.0
33	38.12140910058038	40.0	38.0	41.0	33.0	41.0
34	38.0679313002762	40.0	38.0	41.0	33.0	41.0
35	38.01375282038227	40.0	38.0	41.0	33.0	41.0
36	37.94803783669785	40.0	38.0	41.0	33.0	41.0
37	37.87710702406579	40.0	38.0	41.0	33.0	41.0
38	37.806511324870776	40.0	38.0	41.0	33.0	41.0
39	37.746186391723164	40.0	38.0	41.0	33.0	41.0
40	37.64476557218983	40.0	38.0	41.0	32.0	41.0
41	37.83960939316687	40.0	38.0	41.0	33.0	41.0
42	37.87893092803182	40.0	38.0	41.0	33.0	41.0
43	37.86523727674186	40.0	38.0	41.0	33.0	41.0
44	37.82481105625912	40.0	38.0	41.0	33.0	41.0
45	37.769537347117016	40.0	38.0	41.0	33.0	41.0
46	37.72049565267449	40.0	38.0	41.0	33.0	41.0
47	37.66450862873692	40.0	38.0	41.0	32.0	41.0
48	37.595102535495776	40.0	37.0	41.0	32.0	41.0
49	37.523033325555424	40.0	37.0	41.0	32.0	41.0
50	37.46146124684905	40.0	37.0	41.0	32.0	41.0
51	35.94534942287429	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	2.0
5	25.0
6	528.0
7	3257.0
8	10715.0
9	17254.0
10	21306.0
11	25875.0
12	34704.0
13	45850.0
14	55951.0
15	62630.0
16	66344.0
17	68017.0
18	69634.0
19	72095.0
20	75798.0
21	80582.0
22	89043.0
23	98378.0
24	111856.0
25	127816.0
26	147296.0
27	172341.0
28	204728.0
29	246918.0
30	300658.0
31	377805.0
32	485851.0
33	647233.0
34	911431.0
35	1334487.0
36	2021281.0
37	3329284.0
38	7612417.0
39	1.850281E7
40	2884.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.29098853729425	27.89412443970164	29.707370376668703	23.10751664633541
2	21.307615569725566	29.213490499620875	31.72953928113159	17.749354649521965
3	21.596021486260817	28.006288752917218	31.38843681001392	19.009252950808044
4	20.976191185354594	26.287967557706892	32.363156114110595	20.372685142827912
5	25.60647591506825	26.435380627396682	29.075964183580215	18.882179273954858
6	20.92421360794217	26.01309314409765	33.568905565901886	19.493787682058294
7	24.525007181729542	26.5119314239218	29.9508248922126	19.012236502136055
8	21.665128810101862	26.530834676381083	32.39527065897098	19.40876585454608
9	21.687352897073612	26.634101247019004	32.29630448089442	19.38224137501296
10	21.686831924920156	27.808341615052512	31.256372907796244	19.24845355223109
11	25.04486266245392	26.532306607000994	28.95569585167942	19.467134878865668
12	20.853397514844012	30.35369989541397	29.117777933408835	19.67512465633319
13	23.250283731997136	26.8685519158762	29.77354975576234	20.10761459636432
14	23.534483926233378	27.179072650942288	28.892101587860303	20.394341834964035
15	22.822837319732564	28.255804559611757	28.399797839302014	20.52156028135366
16	22.994951374371247	27.478767121420816	28.76298491152119	20.763296592686746
17	22.86836811201927	27.545901617053488	28.868143234453726	20.71758703647352
18	22.61238848864016	27.498299102069613	28.96093374908731	20.92837866020292
19	22.237841704186064	27.81403773480045	29.29890949373828	20.649211067275207
20	22.001622306403156	27.847436519194257	29.303091064315602	20.847850110086984
21	22.067561543734936	27.93956153817858	29.20285674452477	20.79002017356171
22	21.86221065412755	28.057669753911508	29.392821719552863	20.687297872408077
23	21.83504062371718	28.142211740195094	29.269992816015765	20.752754820071956
24	21.736223967884815	28.186337632384795	29.217478824819644	20.85995957491075
25	21.773692641291333	28.34391395069379	29.08665910431886	20.79573430369602
26	21.766078394109122	28.298205574879375	29.11455880520159	20.821157225809912
27	21.772229579769366	28.274329522473664	28.96907929428757	20.984361603469402
28	21.766900871316526	28.29398507151053	29.009897117930024	20.929216939242927
29	21.777547959527936	28.338829597116582	28.972641607124366	20.910980836231115
30	21.77893065452089	28.34840112794615	28.821878210175417	21.05079000735754
31	21.907434990406134	28.23814859829564	28.80197562519733	21.052440786100888
32	21.906508594198634	28.32693606736919	28.693326757740937	21.073228580691243
33	21.90071735214213	28.263417926082617	28.672488445984595	21.163376275790654
34	21.949895779403825	28.295422976814237	28.59368426767271	21.160996976109228
35	21.877670765763884	28.284249042518617	28.61891466170986	21.21916553000764
36	21.9075414354906	28.3371810467592	28.573390013867154	21.181887503883047
37	21.988114291625962	28.245908436376666	28.59629059494644	21.169686677050933
38	21.881674003828856	28.297327314818823	28.641140888371137	21.179857792981185
39	21.843700289544845	28.179799896669184	28.693971840869477	21.282527972916498
40	21.92597459068829	28.24006451964219	28.601627494293957	21.232333395375562
41	21.88437656001844	28.193945834543182	28.574099023928618	21.347578581509758
42	21.822921406240557	28.144894688415928	28.616271611855797	21.41591229348772
43	21.88014109409269	28.282532968998435	28.520635128620004	21.31669080828887
44	21.913411717712048	28.26298025045247	28.44249657636378	21.381111455471704
45	21.908720866460644	28.218317791571202	28.511385676229985	21.361575665738172
46	21.83046062757358	28.328435166512815	28.418718143142346	21.422386062771253
47	21.927704907398017	28.30106275889392	28.36088776667162	21.410344567036443
48	21.89143027400496	28.281130821490013	28.385251942459806	21.442186962045216
49	21.896163529401296	28.372178310746122	28.31507583191555	21.416582327937032
50	21.872919269910312	28.31010815821879	28.375446372924813	21.441526198946086
51	21.92106316911836	28.26515320157363	28.389449864797562	21.424333764510447
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.39850518362966
2	39.05697021924753
3	40.605274437068864
4	41.348876328182506
5	44.488655189023106
6	40.41800129000047
7	43.5372436838656
8	41.07389466464794
9	41.069594272086576
10	40.93528547715125
11	44.51199754131959
12	40.528522171177194
13	43.35789832836146
14	43.92882576119741
15	43.34439760108623
16	43.758247967058
17	43.585955148492786
18	43.54076714884308
19	42.88705277146127
20	42.849472416490144
21	42.85758171729665
22	42.54950852653563
23	42.58779544378914
24	42.596183542795565
25	42.569426944987356
26	42.587235619919035
27	42.75659118323877
28	42.69611781055945
29	42.68852879575905
30	42.82972066187843
31	42.959875776507026
32	42.97973717488988
33	43.064093627932785
34	43.11089275551305
35	43.096836295771524
36	43.08942893937365
37	43.157800968676895
38	43.061531796810044
39	43.126228262461346
40	43.15830798606385
41	43.2319551415282
42	43.23883369972828
43	43.19683190238156
44	43.294523173183755
45	43.27029653219881
46	43.25284669034484
47	43.338049474434456
48	43.33361723605018
49	43.31274585733833
50	43.3144454688564
51	43.345396933628805
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20087.0
1	12894.5
2	5702.0
3	8184.0
4	10666.0
5	27531.0
6	44396.0
7	55216.0
8	66036.0
9	49771.0
10	33506.0
11	34010.0
12	34514.0
13	43642.5
14	52771.0
15	78516.5
16	104262.0
17	166593.0
18	228924.0
19	285211.5
20	341499.0
21	396114.5
22	450730.0
23	534184.5
24	617639.0
25	732135.0
26	1006473.0
27	1166315.0
28	1252252.5
29	1338190.0
30	1407608.0
31	1477026.0
32	1582525.0
33	1688024.0
34	1784826.5
35	1881629.0
36	2099870.0
37	2318111.0
38	2406969.0
39	2495827.0
40	2592870.0
41	2689913.0
42	2660695.5
43	2631478.0
44	2604407.0
45	2577336.0
46	2484349.0
47	2391362.0
48	2305541.5
49	2219721.0
50	2107876.0
51	1996031.0
52	1855740.5
53	1715450.0
54	1587221.5
55	1458993.0
56	1324485.0
57	1189977.0
58	1060289.5
59	930602.0
60	816159.5
61	701717.0
62	599461.5
63	497206.0
64	422399.5
65	347593.0
66	291562.0
67	235531.0
68	205490.5
69	175450.0
70	152006.5
71	128563.0
72	108581.5
73	88600.0
74	73957.0
75	50237.0
76	41160.0
77	37035.0
78	32910.0
79	31204.5
80	29499.0
81	25808.0
82	22117.0
83	19155.5
84	16194.0
85	14110.5
86	12027.0
87	12768.5
88	13510.0
89	9565.5
90	5621.0
91	3918.0
92	2215.0
93	1762.5
94	1310.0
95	1003.0
96	696.0
97	545.0
98	394.0
99	252.0
100	110.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.1788361105631257E-4
2	0.0
3	0.0
4	0.0
5	0.0019927829735126817
6	0.0026445779407205834
7	0.02739675894952556
8	0.0025323837250536495
9	0.006774393593603434
10	0.0032883590353808465
11	0.009680758037546863
12	0.027760054505018485
13	0.005003327760575407
14	0.006368357384523102
15	0.009288078282712595
16	0.03266187321332381
17	0.003691723953611966
18	0.0011913957187488688
19	0.023747775649500997
20	0.0052330587736077
21	0.0011913957187488688
22	0.02446368159709
23	0.0011379699017646148
24	0.03149719040306707
25	0.0036970665353103912
26	0.0322398092591482
27	0.0312086909913521
28	0.003090683512539106
29	0.03795904296731262
30	0.003694395244461179
31	0.0033765116334048663
32	0.00996391486756341
33	0.011686897465305608
34	0.015554926614965613
35	0.01784689416359012
36	0.016994752382691262
37	0.02123943354209026
38	0.03178034723308362
39	0.02062770793762055
40	0.023942779881493525
41	0.025767271531505806
42	0.017200441778080645
43	0.023274957169190345
44	0.009061018560529514
45	0.028449247544115368
46	0.03997052497676979
47	0.02028311141807211
48	0.04317874528667425
49	0.020403319506286682
50	0.02623207613926882
51	0.10006655521150813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.7435085E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	39.01885177535077
#Duplication Level	Relative count
1	100.0
2	16.004153388950552
3	5.8777614665759605
4	3.0785205342117514
5	1.8754699416377243
6	1.2517454975115472
7	0.9158938737512979
8	0.7010634107916502
9	0.5900676715958323
10++	12.925632854738803
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	14309870	3.7675948	4.70764	17
TGTGT	7258025	3.3687313	34.099182	4
AAAAA	9059290	3.0273597	3.2164752	44
GTTTG	6022735	2.7953846	33.782124	7
TGTTT	7568410	2.6457136	26.029331	6
GGGGG	2370555	2.5752945	5.468358	13
GTGTT	5389855	2.5016403	33.631874	5
CTTTG	4897210	2.4237375	35.98837	1
TTTGA	6555850	2.4036734	26.878368	8
GAGGG	2655540	2.278916	11.030954	11
TTGTG	4890525	2.2698822	33.558655	3
TTTGT	5781020	2.0208898	25.618006	2
TTGAG	4097515	1.9946958	21.325865	9
TGAGG	2956890	1.9111772	13.088755	10
AGGGG	1959405	1.6815108	5.933628	12
AACTT	3881280	1.5915442	11.109361	2
TGAGC	2148265	1.4806166	5.957329	10
ACTTT	3709900	1.4504329	10.097102	3
TGAGT	2437925	1.186797	6.2186136	10
TTGAT	3026010	1.1094731	6.195456	9
TAACT	2663130	1.0920337	10.769517	1
TTGAC	1971765	1.0235293	5.6665893	9
>>END_MODULE