FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_12_CCGTCC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_12_CCGTCC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 37435085
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 13453800 3.5766995 4.386638 18
TGTGT 6079395 2.9305394 37.484352 4
GTTTG 4891135 2.3577454 36.89805 7
TGTTT 6468625 2.3156579 28.03835 6
CTTTG 4436025 2.2280166 38.471992 1
GAGGG 2375905 2.1533985 12.139489 11
GTGTT 4261540 2.0542524 36.843647 5
TTTGA 5505285 2.0436223 28.649565 8
GGGGG 1702570 2.0038571 5.3551984 13
TTTGT 5315985 1.9030321 27.773127 2
TTGTG 3875770 1.8682941 36.839523 3
TGAGG 2656245 1.7878811 14.495948 10
TTGAG 3434245 1.7166309 23.040833 9
AGGGG 1727020 1.5652825 6.530131 12
TGAGC 2024580 1.4198507 6.175108 10
TGAGT 2292805 1.1460743 6.7039304 10
GAGTG 1673400 1.1263419 5.201572 11
TTGAT 2807440 1.0421525 6.6542892 9
TTGAC 1862500 0.97001654 5.923907 9
Produced by FastQC (version 0.10.1)