##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_12_CCGTCC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	37435085	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.547956148623676	34.0	31.0	34.0	31.0	34.0
2	32.82736684049201	34.0	31.0	34.0	31.0	34.0
3	32.92303156250347	34.0	31.0	34.0	31.0	34.0
4	36.37830564562629	37.0	37.0	37.0	35.0	37.0
5	36.28814234026716	37.0	37.0	37.0	35.0	37.0
6	36.255803479543324	37.0	37.0	37.0	35.0	37.0
7	36.26425493624497	37.0	37.0	37.0	35.0	37.0
8	36.233480517006974	37.0	37.0	37.0	35.0	37.0
9	37.982776131001174	39.0	38.0	39.0	35.0	39.0
10	37.97208469007082	39.0	38.0	39.0	35.0	39.0
11	37.966749587986776	39.0	38.0	39.0	35.0	39.0
12	37.93319528992655	39.0	38.0	39.0	35.0	39.0
13	37.93487713464521	39.0	38.0	39.0	35.0	39.0
14	39.425969461535885	41.0	39.0	41.0	37.0	41.0
15	39.43114006552943	41.0	39.0	41.0	37.0	41.0
16	39.4174241623867	41.0	39.0	41.0	37.0	41.0
17	39.39466949253621	41.0	39.0	41.0	36.0	41.0
18	39.34761267404629	41.0	39.0	41.0	36.0	41.0
19	39.36072713605432	41.0	39.0	41.0	36.0	41.0
20	39.33758563657596	41.0	39.0	41.0	36.0	41.0
21	39.31406240963524	40.0	39.0	41.0	36.0	41.0
22	39.29364308909677	40.0	39.0	41.0	36.0	41.0
23	39.276437652004795	40.0	39.0	41.0	36.0	41.0
24	39.24075390773121	40.0	39.0	41.0	36.0	41.0
25	39.19412962465559	40.0	39.0	41.0	36.0	41.0
26	39.12489633721948	40.0	39.0	41.0	36.0	41.0
27	39.04262816552974	40.0	39.0	41.0	36.0	41.0
28	39.00181944291031	40.0	39.0	41.0	36.0	41.0
29	38.92487114694678	40.0	39.0	41.0	35.0	41.0
30	38.84073379291112	40.0	38.0	41.0	35.0	41.0
31	38.78911857686446	40.0	38.0	41.0	35.0	41.0
32	38.72937633238979	40.0	38.0	41.0	35.0	41.0
33	38.6522255792928	40.0	38.0	41.0	35.0	41.0
34	38.78955346301471	40.0	38.0	41.0	35.0	41.0
35	38.80698112479242	40.0	38.0	41.0	35.0	41.0
36	38.79083474767054	40.0	38.0	41.0	35.0	41.0
37	38.765811484066354	40.0	38.0	41.0	35.0	41.0
38	38.729740108777634	40.0	38.0	41.0	35.0	41.0
39	38.6483505246482	40.0	38.0	41.0	35.0	41.0
40	38.601458604942394	40.0	38.0	41.0	35.0	41.0
41	38.5650047809428	40.0	38.0	41.0	35.0	41.0
42	37.93157651438484	40.0	38.0	41.0	33.0	41.0
43	38.17672429486937	40.0	38.0	41.0	33.0	41.0
44	38.28365299557888	40.0	38.0	41.0	34.0	41.0
45	38.28612794655068	40.0	38.0	41.0	34.0	41.0
46	38.25133919156321	40.0	38.0	41.0	34.0	41.0
47	38.18769926661045	40.0	38.0	41.0	34.0	41.0
48	38.12739543666056	40.0	38.0	41.0	34.0	41.0
49	37.9578677061906	40.0	38.0	41.0	33.0	41.0
50	38.1145275347979	40.0	38.0	41.0	34.0	41.0
51	37.896300943353005	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	4.0
10	19.0
11	38.0
12	85.0
13	134.0
14	249.0
15	404.0
16	636.0
17	1089.0
18	1947.0
19	3475.0
20	6464.0
21	11813.0
22	19784.0
23	31683.0
24	47303.0
25	68080.0
26	94479.0
27	127882.0
28	170605.0
29	223256.0
30	289968.0
31	374786.0
32	486768.0
33	642980.0
34	895230.0
35	1320558.0
36	1992088.0
37	3346294.0
38	7620807.0
39	1.9646705E7
40	9469.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.304174083465853	29.069382549831296	28.591284572250753	24.035158794452098
2	20.139086027096436	28.504239659819604	32.561959928314074	18.794714384769897
3	19.057384850032427	27.336939452046664	33.767874903704254	19.837800794216655
4	19.97135791454746	27.2609017494639	33.24736111109998	19.52037922488866
5	24.954945340714467	27.330198395435723	28.78330849255451	18.931547771295296
6	21.372260808276515	26.967912587883795	31.903483056068925	19.75634354777076
7	24.912880523711912	27.602990616957328	28.075958689555534	19.408170169775225
8	20.82505756297869	27.410476028036268	31.87584053836127	19.888625870623773
9	20.368899923694578	27.405082691811707	32.35101776849178	19.87499961600194
10	20.738777539839965	27.442980295089487	32.09531646582344	19.72292569924711
11	24.58579137363797	27.32843660742472	28.28126758569571	19.804504433241604
12	20.691340756939645	31.33452481809511	28.03624193720944	19.937892487755803
13	22.987096195988336	27.683476610244107	28.95348040481276	20.3759467889548
14	22.287119155733183	27.847296192862924	29.243371559060172	20.62221309234372
15	22.459339948072778	27.8054717920368	28.959525536004527	20.775662723885898
16	22.24203845136187	27.816520250989146	29.01634389236728	20.925097405281704
17	22.369336573523878	27.931341015984874	28.838191860403768	20.861130550087488
18	22.083828501932153	27.905664832065114	29.041501410853172	20.96900525514956
19	21.722515121843585	28.237483633334875	29.350760122489373	20.689241122332167
20	21.525101385505067	28.243988226552712	29.34452800093816	20.886382387004062
21	21.691271704071195	28.222361455837486	29.16970804260228	20.916658797489042
22	21.580215458306025	28.247028155539116	29.316671779962565	20.856084606192294
23	21.653860275727972	28.30238531580735	29.161207995120087	20.882546413344592
24	21.504885056358226	28.33631070959235	29.153418511003782	21.00538572304564
25	21.580549369662176	28.480344040891055	29.01943457587982	20.919672013566952
26	21.611969092630616	28.42696630714208	28.98900323052559	20.972061369701713
27	21.65512241439714	28.399146412301775	28.85513758738996	21.090593585911126
28	21.620829764377454	28.389667073014525	28.96512723291533	21.02437592969269
29	21.641927619504536	28.47314224076157	28.891359536114315	20.99357060361957
30	21.650945897411482	28.40058463871526	28.830948293559373	21.117521170313893
31	21.765558481008952	28.332746494440116	28.774803003379212	21.12689202117172
32	21.771193010039898	28.46090427423849	28.64508510483078	21.122817610890827
33	21.77660422292755	28.383724440815755	28.620620533021285	21.21905080323541
34	21.774965876191768	28.408529481509113	28.587845595968503	21.228659046330616
35	21.697912582066326	28.429577035686005	28.58381047609347	21.2886999061542
36	21.798662416756333	28.470310362577056	28.505261771063516	21.225765449603095
37	21.86453964242368	28.380175976627275	28.539117782155433	21.216166598793617
38	21.74998725794231	28.40793144740907	28.606095821769763	21.235985472878863
39	21.726992205306868	28.32470395085252	28.636782846893492	21.311520996947113
40	21.74845335598944	28.41035622064168	28.57396209999256	21.26722832337632
41	21.759310410413512	28.309049100240312	28.567848216775783	21.363792272570393
42	21.874497712259235	28.368065610956727	28.408225924174047	21.349210752609988
43	21.7827524671289	28.402956034847804	28.473376741538107	21.340914756485184
44	21.836491088506946	28.33273117985441	28.429955481602352	21.400822250036295
45	21.83871093120264	28.254879079344953	28.53101308571892	21.37539690373349
46	21.723410004278072	28.400691490349228	28.46351223725016	21.41238626812254
47	21.82607420085588	28.440846586272123	28.374313743986164	21.35876546888583
48	21.77481853131253	28.39033734341062	28.4053437419017	21.429500383375142
49	21.896098272516276	28.47420007193786	28.2882969278686	21.341404727677258
50	21.756079760994766	28.388951669183264	28.387394073114518	21.46757449670745
51	21.84073245024317	28.346935009778534	28.3467960512805	21.465536488697797
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.339332877917954
2	38.93380041186633
3	38.89518564424908
4	39.491737139436125
5	43.88649311200976
6	41.128604356047276
7	44.32105069348714
8	40.71368343360246
9	40.243899539696514
10	40.46170323908708
11	44.39029580687957
12	40.62923324469545
13	43.363042984943135
14	42.9093322480769
15	43.235002671958675
16	43.167135856643576
17	43.23046712361137
18	43.052833757081714
19	42.411756244175756
20	42.41148377250913
21	42.60793050156023
22	42.43630006449832
23	42.536406689072564
24	42.51027077940387
25	42.50022138322913
26	42.58403046233233
27	42.74571600030827
28	42.64520569407015
29	42.63549822312411
30	42.76846706772537
31	42.89245050218067
32	42.89401062093072
33	42.995655026162964
34	43.00362492252239
35	42.98661248822052
36	43.024427866359424
37	43.0807062412173
38	42.98597273082117
39	43.03851320225398
40	43.015681679365755
41	43.123102682983905
42	43.22370846486922
43	43.123667223614085
44	43.237313338543245
45	43.21410783493613
46	43.13579627240061
47	43.184839669741706
48	43.204318914687676
49	43.237503000193534
50	43.22365425770221
51	43.30626893894097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33798.0
1	20581.5
2	7365.0
3	9846.5
4	12328.0
5	18010.5
6	23693.0
7	40583.0
8	57473.0
9	43692.5
10	29912.0
11	31402.0
12	32892.0
13	42873.5
14	52855.0
15	82277.0
16	111699.0
17	179307.0
18	246915.0
19	308414.5
20	369914.0
21	424191.5
22	478469.0
23	561677.0
24	644885.0
25	770653.0
26	1065495.5
27	1234570.0
28	1307646.0
29	1380722.0
30	1440670.0
31	1500618.0
32	1601719.0
33	1702820.0
34	1798694.0
35	1894568.0
36	2109711.0
37	2324854.0
38	2401403.5
39	2477953.0
40	2565831.5
41	2653710.0
42	2619805.0
43	2585900.0
44	2561970.5
45	2538041.0
46	2446472.5
47	2354904.0
48	2276248.0
49	2197592.0
50	2085038.0
51	1972484.0
52	1835916.5
53	1699349.0
54	1575852.5
55	1452356.0
56	1319998.0
57	1187640.0
58	1056138.5
59	924637.0
60	810757.0
61	696877.0
62	594599.5
63	492322.0
64	416286.5
65	340251.0
66	284623.0
67	228995.0
68	199888.0
69	170781.0
70	147888.0
71	124995.0
72	105578.5
73	86162.0
74	71259.5
75	46549.5
76	36742.0
77	33653.5
78	30565.0
79	29161.5
80	27758.0
81	24307.0
82	20856.0
83	17594.5
84	14333.0
85	12283.0
86	10233.0
87	11217.5
88	12202.0
89	7780.0
90	3358.0
91	1996.0
92	634.0
93	443.0
94	252.0
95	149.0
96	46.0
97	33.0
98	20.0
99	14.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026475163606547177
2	3.312400653023761E-4
3	0.005946293430347493
4	0.04279942198608605
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.006192052188475063
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.008361140358035783
18	0.004559893479606097
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	4.006936273819066E-4
28	0.0
29	0.0
30	0.0
31	1.3890712415906095E-4
32	3.6329555549292864E-4
33	2.8582812086576E-4
34	2.0836068623859141E-4
35	7.265911109858573E-4
36	6.838504573984539E-4
37	0.0
38	2.6712908492127106E-6
39	0.0
40	0.0
41	0.001722982597742198
42	3.152123202070998E-4
43	1.9500423199252787E-4
44	0.0
45	0.0
46	0.0
47	0.006528634835475864
48	0.011713610373797735
49	0.0
50	0.01499128424578173
51	0.036970665353103914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.7435085E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	40.82491582491582
#Duplication Level	Relative count
1	100.0
2	16.95663274610643
3	6.444906444906445
4	3.229383229383229
5	2.0264799212167635
6	1.3342087026297553
7	1.0205347047452311
8	0.7360396834081044
9	0.60838166101324
10++	13.538315643578802
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13453800	3.5766995	4.386638	18
TGTGT	6079395	2.9305394	37.484352	4
GTTTG	4891135	2.3577454	36.89805	7
TGTTT	6468625	2.3156579	28.03835	6
CTTTG	4436025	2.2280166	38.471992	1
GAGGG	2375905	2.1533985	12.139489	11
GTGTT	4261540	2.0542524	36.843647	5
TTTGA	5505285	2.0436223	28.649565	8
GGGGG	1702570	2.0038571	5.3551984	13
TTTGT	5315985	1.9030321	27.773127	2
TTGTG	3875770	1.8682941	36.839523	3
TGAGG	2656245	1.7878811	14.495948	10
TTGAG	3434245	1.7166309	23.040833	9
AGGGG	1727020	1.5652825	6.530131	12
TGAGC	2024580	1.4198507	6.175108	10
TGAGT	2292805	1.1460743	6.7039304	10
GAGTG	1673400	1.1263419	5.201572	11
TTGAT	2807440	1.0421525	6.6542892	9
TTGAC	1862500	0.97001654	5.923907	9
>>END_MODULE