FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_11_ACTTGA_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_11_ACTTGA_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34870697
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 7979215 4.030419 28.710936 4
TTTTT 11199385 3.2984512 4.0267262 17
TGTTT 7022435 2.7085738 21.962069 6
GTTTG 5348630 2.7016716 27.892513 7
GGGGG 2269410 2.5746257 5.1072345 13
GTGTT 5007010 2.5291142 27.830645 5
CTTTG 4581090 2.4797542 29.93785 1
TTTGA 5841390 2.3350494 22.274078 8
TTGTG 4605480 2.3262956 27.844053 3
GAGGG 2489100 2.2347727 9.041755 11
TTTGT 5472645 2.1108153 21.527899 2
TGAGG 2934450 2.0117807 11.067769 10
TTGAG 3801445 1.9900575 17.846224 9
AGGGG 1944700 1.7459974 5.3778806 12
ACTTT 3453130 1.4792534 11.994165 3
AACTT 3244510 1.440475 12.815001 2
TGAGT 2513775 1.3159618 5.712462 10
TAACT 2439865 1.0832343 12.593464 1
TTGAT 2572975 1.0285263 5.086023 9