##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_11_ACTTGA_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34870697	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.487903037900274	34.0	31.0	34.0	31.0	34.0
2	32.64327707587835	34.0	31.0	34.0	31.0	34.0
3	32.71247408676689	34.0	31.0	34.0	31.0	34.0
4	36.109451038503764	37.0	37.0	37.0	35.0	37.0
5	36.12883860623721	37.0	37.0	37.0	35.0	37.0
6	36.140904955240785	37.0	37.0	37.0	35.0	37.0
7	36.14298460968532	37.0	37.0	37.0	35.0	37.0
8	36.15013149866204	37.0	37.0	37.0	35.0	37.0
9	37.92410788347592	39.0	39.0	39.0	37.0	39.0
10	37.919973179773265	39.0	39.0	39.0	37.0	39.0
11	37.89838052276386	39.0	39.0	39.0	37.0	39.0
12	37.86027959234655	39.0	39.0	39.0	35.0	39.0
13	37.85960174527054	39.0	38.0	39.0	35.0	39.0
14	39.39167410390449	41.0	40.0	41.0	37.0	41.0
15	39.35406387202412	41.0	40.0	41.0	37.0	41.0
16	39.321057849804376	41.0	40.0	41.0	37.0	41.0
17	39.29182539712355	41.0	40.0	41.0	37.0	41.0
18	39.25942767934922	41.0	40.0	41.0	36.0	41.0
19	39.28075931490558	41.0	40.0	41.0	37.0	41.0
20	39.251116747107176	41.0	40.0	41.0	37.0	41.0
21	39.218250756501945	41.0	40.0	41.0	36.0	41.0
22	39.15643111464047	41.0	39.0	41.0	36.0	41.0
23	39.135716501451064	41.0	39.0	41.0	36.0	41.0
24	39.0954057213138	41.0	39.0	41.0	36.0	41.0
25	39.06996209453456	41.0	39.0	41.0	36.0	41.0
26	39.01484891454851	41.0	39.0	41.0	36.0	41.0
27	38.9641166335161	41.0	39.0	41.0	36.0	41.0
28	38.920764015700634	41.0	39.0	41.0	36.0	41.0
29	38.84682729456196	40.0	39.0	41.0	35.0	41.0
30	38.80517487218567	40.0	39.0	41.0	35.0	41.0
31	38.77934748479504	40.0	39.0	41.0	35.0	41.0
32	38.71278385975479	40.0	39.0	41.0	35.0	41.0
33	38.65995755691376	40.0	39.0	41.0	35.0	41.0
34	38.621791700922984	40.0	38.0	41.0	35.0	41.0
35	38.565525289041396	40.0	38.0	41.0	35.0	41.0
36	38.516940140313224	40.0	38.0	41.0	35.0	41.0
37	38.466225180414376	40.0	38.0	41.0	34.0	41.0
38	38.39041224211836	40.0	38.0	41.0	34.0	41.0
39	38.33778670956878	40.0	38.0	41.0	34.0	41.0
40	38.25994367132954	40.0	38.0	41.0	34.0	41.0
41	38.455861579136204	40.0	38.0	41.0	35.0	41.0
42	38.49185664398965	40.0	38.0	41.0	35.0	41.0
43	38.47640759804715	40.0	38.0	41.0	35.0	41.0
44	38.44766463945358	40.0	38.0	41.0	34.0	41.0
45	38.41770234761869	40.0	38.0	41.0	34.0	41.0
46	38.36841024428046	40.0	38.0	41.0	34.0	41.0
47	38.31959969139705	40.0	38.0	41.0	34.0	41.0
48	38.24143248412844	40.0	38.0	41.0	34.0	41.0
49	38.19697722130418	40.0	38.0	41.0	34.0	41.0
50	38.105727482304125	40.0	38.0	41.0	33.0	41.0
51	36.64219149390676	38.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	98.0
5	242.0
6	1051.0
7	3873.0
8	12054.0
9	16627.0
10	15894.0
11	17051.0
12	22175.0
13	29218.0
14	35962.0
15	40889.0
16	44042.0
17	45534.0
18	46780.0
19	48323.0
20	50584.0
21	53414.0
22	58288.0
23	65068.0
24	73630.0
25	84247.0
26	97173.0
27	113790.0
28	135988.0
29	163803.0
30	200220.0
31	254036.0
32	328808.0
33	441259.0
34	632510.0
35	951465.0
36	1458335.0
37	2524399.0
38	6296204.0
39	2.0502097E7
40	5557.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.831801540995624	29.026906264163248	28.613719115393476	22.527573079447656
2	21.96846225232263	30.124970031511367	29.810679450162837	18.095888266003175
3	21.557700184599227	28.718174872257286	30.5967848000198	19.127340143123693
4	21.31874029370572	26.588468378953	31.40026384544789	20.69252748189339
5	24.66370043201891	26.734244134387225	29.5264482246067	19.07560720898717
6	21.06567272888075	26.21602678222306	33.00022457318119	19.718075915714998
7	23.955747751154867	26.572306955133705	30.353214681185293	19.118730612526132
8	22.407767319986473	26.733649253540204	31.314550571750793	19.54403285472253
9	21.68539205955534	27.014003229210992	31.829644721394217	19.470959989839447
10	22.28203184143496	27.66940066862688	30.676905124542785	19.371662365395373
11	24.42706331393918	26.633124362229697	29.41639218360821	19.523420140222917
12	21.06105482685557	29.709379122832953	29.55581950482207	19.6737465454894
13	22.923927433768885	26.981066719662326	30.061611468607772	20.03339437796102
14	23.647459253741037	27.285301360223794	28.839454038330015	20.227785347705158
15	22.715391572898575	28.63745616964207	28.26280252558308	20.38434973187627
16	23.157825638238407	27.643557951371122	28.5724446220445	20.626171788345975
17	22.97056239477013	27.648035658431752	28.718860089307835	20.662541857490286
18	22.798165011319256	27.55147354560662	28.797380682028063	20.85298076104606
19	22.513779221140176	27.821838007167855	28.97319149452458	20.69119127716739
20	22.322903342109974	27.873375228578702	28.99902173305816	20.804699696253163
21	22.27990379082592	27.951487830574205	28.962720608411637	20.80588777018823
22	22.11490312151343	28.133404070484147	29.02414715622168	20.72754565178074
23	22.082418652805515	28.167562474200214	28.978065794585184	20.77195307840909
24	21.989238966612564	28.231390048583314	28.92136033279843	20.858010652005692
25	22.008708418714555	28.297390597625494	28.841763561909083	20.852137421750868
26	21.988065832738197	28.314470401536152	28.831857443517478	20.865606322208173
27	21.986329864121377	28.296850682678894	28.735476990793206	20.981342462406523
28	21.9688838609102	28.29701281565739	28.744901893947922	20.989201429484485
29	21.99121294350722	28.324059057340477	28.687898025555604	20.996829973596697
30	21.978719631242043	28.318339533244803	28.61976886056684	21.083171974946314
31	22.05379045624104	28.301371058351737	28.56185066014011	21.082987825267114
32	22.04216975156936	28.337732919054194	28.51925617380032	21.100841155576127
33	22.03283951440313	28.303461957116966	28.498991672166174	21.164706856313728
34	22.04705885390304	28.320427171972202	28.465237988621045	21.167275985503714
35	22.03888350737163	28.36058766761224	28.440690419870872	21.159838405145255
36	22.000405499732818	28.328613449908147	28.45354167074781	21.21743937961122
37	22.03827711041486	28.301570641945222	28.44134910452612	21.218803143113796
38	21.97183383069852	28.332343422921802	28.471961818052765	21.22386092832691
39	21.9667280546794	28.278795791432813	28.454762268283005	21.29971388560478
40	22.00616861435894	28.301001041932466	28.414014546884193	21.278815796824404
41	22.030614213157932	28.281108089096296	28.396397197437796	21.29188050030798
42	21.969175462640813	28.259634682729732	28.412656612010622	21.35853324261883
43	22.021827504765255	28.305134067427435	28.35750678987745	21.315531637929855
44	22.01406821475332	28.336940159705794	28.32972354306162	21.319268082479272
45	22.012988702640772	28.290215193218376	28.34028561398176	21.356510490159096
46	21.96095237565208	28.331545069610325	28.321779950558202	21.385722604179392
47	22.010062525656537	28.32470581459464	28.302918552352875	21.362313107395945
48	21.963278913136676	28.3067839321925	28.30226483228128	21.427672322389547
49	21.959879282288597	28.338421369476443	28.28049256350857	21.42120678472639
50	21.956166406970617	28.34426152102519	28.283671038659335	21.41590103334486
51	21.99382788262295	28.28479090970332	28.291613594164332	21.429767613509398
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.359374620443276
2	40.0643505183258
3	40.685040327722916
4	42.01126777559911
5	43.73930764100608
6	40.78374864459575
7	43.074478363680996
8	41.951800174709
9	41.15635204939479
10	41.65369420683033
11	43.9504834541621
12	40.734801372344975
13	42.957321811729905
14	43.87524460144619
15	43.099741304774845
16	43.78399742658438
17	43.63310425226041
18	43.65114577236531
19	43.20497049830757
20	43.12760303836314
21	43.085791561014155
22	42.84244877329417
23	42.8543717312146
24	42.84724961861826
25	42.86084584046542
26	42.85367215494637
27	42.967672326527904
28	42.958085290394685
29	42.98804291710392
30	43.061891606188354
31	43.13677828150816
32	43.14301090714549
33	43.19754637071686
34	43.21433483940675
35	43.19872191251689
36	43.21784487934404
37	43.257080253528656
38	43.195694759025436
39	43.26644194028418
40	43.284984411183345
41	43.32249471346591
42	43.327708705259646
43	43.337359142695114
44	43.33333629723259
45	43.369499192799864
46	43.34667497983148
47	43.37237563305248
48	43.390951235526224
49	43.38108606701499
50	43.372067440315476
51	43.42359549613235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18349.0
1	11742.0
2	5135.0
3	6481.0
4	7827.0
5	17031.5
6	26236.0
7	33042.5
8	39849.0
9	33317.0
10	26785.0
11	30131.0
12	33477.0
13	43367.0
14	53257.0
15	72748.0
16	92239.0
17	131132.5
18	170026.0
19	215084.0
20	260142.0
21	313345.0
22	366548.0
23	441794.0
24	517040.0
25	614641.0
26	832583.0
27	952924.0
28	1056734.5
29	1160545.0
30	1261652.0
31	1362759.0
32	1479150.5
33	1595542.0
34	1708485.0
35	1821428.0
36	1974975.5
37	2128523.0
38	2225360.5
39	2322198.0
40	2404999.5
41	2487801.0
42	2518128.0
43	2548455.0
44	2548373.5
45	2548292.0
46	2495104.0
47	2441916.0
48	2354834.0
49	2267752.0
50	2143190.0
51	2018628.0
52	1867466.0
53	1716304.0
54	1560420.5
55	1404537.0
56	1251748.5
57	1098960.0
58	963999.0
59	829038.0
60	712949.5
61	596861.0
62	501240.5
63	405620.0
64	337101.0
65	268582.0
66	220627.5
67	172673.0
68	144095.5
69	115518.0
70	97337.0
71	79156.0
72	66227.5
73	53299.0
74	45039.5
75	31195.0
76	25610.0
77	22732.5
78	19855.0
79	18556.0
80	17257.0
81	14972.5
82	12688.0
83	11290.5
84	9893.0
85	8557.5
86	7222.0
87	7352.0
88	7482.0
89	5573.5
90	3665.0
91	2686.0
92	1707.0
93	1366.5
94	1026.0
95	826.5
96	627.0
97	478.0
98	329.0
99	211.0
100	93.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00780024557581972
2	0.0021135224225658582
3	5.7354746881027355E-5
4	8.115696683665371E-4
5	2.867737344051368E-6
6	2.982446837813423E-4
7	0.02843361576626931
8	0.013535720263922456
9	0.018505509081163475
10	0.0166644217062825
11	0.0174014302037037
12	0.015517326768661951
13	0.014189564378366167
14	0.004958317867864815
15	0.008677773203099438
16	0.045462240115246334
17	0.00855446049730523
18	0.007711345718154129
19	0.01842808017287409
20	0.002492063751980639
21	0.004797724576597938
22	0.026503628533722744
23	0.017392826991671544
24	0.049230446985329836
25	0.008769540798109083
26	0.02132162715302192
27	0.06737462116114284
28	0.010272235166392
29	0.08054040330768267
30	0.05946826930359322
31	0.026480686634970333
32	0.04016552924078346
33	0.028708918551298243
34	0.030776557176359277
35	0.015709465170713394
36	0.014556634758404742
37	0.01584711656322786
38	0.044997666665510014
39	0.019127808084822624
40	0.054802460644821645
41	0.017699674887485042
42	0.010894534170051148
43	0.0423450096222625
44	0.019253988527960883
45	0.03954609797446836
46	0.03421497425187687
47	0.018035200156739054
48	0.05340874029561267
49	0.020684989462642515
50	0.09145787937648622
51	0.13104125793642724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4870697E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.832907610005304
#Duplication Level	Relative count
1	100.0
2	11.614849419788401
3	3.369474249597162
4	1.5026793409695591
5	0.8849944414604595
6	0.5827098192537817
7	0.4665425884057609
8	0.34975080255318086
9	0.31540027730242204
10++	5.82459997251958
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	7979215	4.030419	28.710936	4
TTTTT	11199385	3.2984512	4.0267262	17
TGTTT	7022435	2.7085738	21.962069	6
GTTTG	5348630	2.7016716	27.892513	7
GGGGG	2269410	2.5746257	5.1072345	13
GTGTT	5007010	2.5291142	27.830645	5
CTTTG	4581090	2.4797542	29.93785	1
TTTGA	5841390	2.3350494	22.274078	8
TTGTG	4605480	2.3262956	27.844053	3
GAGGG	2489100	2.2347727	9.041755	11
TTTGT	5472645	2.1108153	21.527899	2
TGAGG	2934450	2.0117807	11.067769	10
TTGAG	3801445	1.9900575	17.846224	9
AGGGG	1944700	1.7459974	5.3778806	12
ACTTT	3453130	1.4792534	11.994165	3
AACTT	3244510	1.440475	12.815001	2
TGAGT	2513775	1.3159618	5.712462	10
TAACT	2439865	1.0832343	12.593464	1
TTGAT	2572975	1.0285263	5.086023	9
>>END_MODULE