FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_11_ACTTGA_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_11_ACTTGA_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34870697
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 6682530 3.5089278 31.486591 4
TTTTT 10738835 3.1661985 3.8038151 18
TGTTT 5961660 2.3457108 23.49979 6
GTTTG 4231295 2.2218096 30.431524 7
CTTTG 4018840 2.2014697 31.7716 1
GAGGG 2183675 2.0956173 10.061972 11
GTGTT 3882240 2.0385246 30.401821 5
TTTGA 4788315 1.9334273 23.635399 8
TTTGT 4905785 1.9302598 23.118189 2
TTGTG 3628190 1.9051253 30.452665 3
TGAGG 2629960 1.8912406 12.376528 10
TTGAG 3156220 1.7007428 19.493843 9
AGGGG 1707420 1.6385676 5.9570775 12
GAGTG 1796955 1.2922153 5.22613 11
TGAGT 2343105 1.2625923 6.1819854 10
TTGAT 2366545 0.9555643 5.3745365 9
Produced by FastQC (version 0.10.1)