##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_11_ACTTGA_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34870697	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76628534267611	34.0	31.0	34.0	31.0	34.0
2	33.00644959864152	34.0	31.0	34.0	31.0	34.0
3	33.08719556136202	34.0	33.0	34.0	31.0	34.0
4	36.4935203618098	37.0	37.0	37.0	35.0	37.0
5	36.41828272030238	37.0	37.0	37.0	35.0	37.0
6	36.40497977427867	37.0	37.0	37.0	35.0	37.0
7	36.4099225776875	37.0	37.0	37.0	35.0	37.0
8	36.39217896906391	37.0	37.0	37.0	35.0	37.0
9	38.20260056746213	39.0	39.0	39.0	37.0	39.0
10	38.204533365077275	39.0	39.0	39.0	37.0	39.0
11	38.1963548362684	39.0	39.0	39.0	37.0	39.0
12	38.16618672692433	39.0	39.0	39.0	37.0	39.0
13	38.14667320816673	39.0	38.0	39.0	37.0	39.0
14	39.71075786067597	41.0	40.0	41.0	38.0	41.0
15	39.7108226141852	41.0	40.0	41.0	38.0	41.0
16	39.68671343162426	41.0	40.0	41.0	38.0	41.0
17	39.67561256375231	41.0	40.0	41.0	37.0	41.0
18	39.66165238968409	41.0	40.0	41.0	37.0	41.0
19	39.67907157118196	41.0	40.0	41.0	37.0	41.0
20	39.661722563216905	41.0	40.0	41.0	37.0	41.0
21	39.643133459592164	41.0	40.0	41.0	37.0	41.0
22	39.61634612580299	41.0	40.0	41.0	37.0	41.0
23	39.587392847352604	41.0	40.0	41.0	37.0	41.0
24	39.56319166777767	41.0	40.0	41.0	37.0	41.0
25	39.518147170961335	41.0	39.0	41.0	37.0	41.0
26	39.45723608564521	41.0	39.0	41.0	37.0	41.0
27	39.389692583431874	41.0	39.0	41.0	37.0	41.0
28	39.33319939088112	41.0	39.0	41.0	36.0	41.0
29	39.28565414106865	41.0	39.0	41.0	36.0	41.0
30	39.238809307425086	40.0	39.0	41.0	36.0	41.0
31	39.17835115254507	40.0	39.0	41.0	36.0	41.0
32	39.09828389722178	40.0	39.0	41.0	36.0	41.0
33	39.050915844899805	40.0	39.0	41.0	36.0	41.0
34	39.18825422388316	40.0	39.0	41.0	36.0	41.0
35	39.21773404758729	40.0	39.0	41.0	36.0	41.0
36	39.20283649621342	40.0	39.0	41.0	36.0	41.0
37	39.18211376732734	40.0	39.0	41.0	36.0	41.0
38	39.1564093771914	40.0	39.0	41.0	36.0	41.0
39	39.100866810892825	40.0	39.0	41.0	36.0	41.0
40	38.9363084425872	40.0	39.0	41.0	35.0	41.0
41	38.96087273506463	40.0	39.0	41.0	35.0	41.0
42	38.805753667613814	40.0	39.0	41.0	35.0	41.0
43	38.778651427586894	40.0	39.0	41.0	35.0	41.0
44	38.81887345125335	40.0	38.0	41.0	35.0	41.0
45	38.81628345427107	40.0	38.0	41.0	35.0	41.0
46	38.754727931018984	40.0	38.0	41.0	35.0	41.0
47	38.71069738009538	40.0	38.0	41.0	35.0	41.0
48	38.67152635348815	40.0	38.0	41.0	35.0	41.0
49	38.627975747086445	40.0	38.0	41.0	35.0	41.0
50	38.72584929977167	40.0	38.0	41.0	35.0	41.0
51	38.45154253727707	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	11.0
11	27.0
12	60.0
13	130.0
14	215.0
15	379.0
16	588.0
17	906.0
18	1503.0
19	2612.0
20	4758.0
21	8359.0
22	13728.0
23	22059.0
24	32599.0
25	46491.0
26	63589.0
27	85640.0
28	114229.0
29	149990.0
30	194598.0
31	252997.0
32	327447.0
33	437193.0
34	618324.0
35	928589.0
36	1420426.0
37	2513516.0
38	6058187.0
39	2.1552477E7
40	19062.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.78467729222079	30.36999074545551	27.41740841087332	23.42792355145038
2	20.57021577384452	29.251160911026513	30.888558506462914	19.290064808666056
3	19.362968562380996	27.787787231432155	32.86164673231465	19.987597473872203
4	20.23063514308429	27.55689444448237	32.5969600415742	19.615510370859138
5	24.33977445303144	27.703641828553067	28.908068571155894	19.048515147259604
6	21.571786180700975	27.23698992095253	31.199931081279153	19.99129281706734
7	24.338191462017523	27.748932004427672	28.36822561935025	19.544650914204553
8	21.202231776439685	27.72251440801427	31.022239102361503	20.053014713184538
9	20.656756588490328	27.759290271714388	31.57938598130115	20.004567158494137
10	21.021971542467305	27.657646189291828	31.41300559607398	19.90737667216689
11	24.060917420542562	27.486746602776513	28.522243987083478	19.93009198959745
12	20.9586031503758	30.708021121573797	28.24911414876508	20.08426157928532
13	22.733064383542434	27.895444705335255	29.000077056102434	20.371413855019878
14	22.197963496428468	27.99735976399055	29.233486945587362	20.57118979399362
15	22.37125630153019	28.004097537826674	28.93362871410342	20.69101744653971
16	22.299201533023556	27.995067032930255	28.88064726667207	20.825084167374115
17	22.35644111697703	28.08242364784959	28.77606325053426	20.785071984639117
18	22.048042228694197	28.02837866991876	29.00765361816542	20.915925483221628
19	21.807596791082208	28.29897836570344	29.120665411419793	20.77275943179455
20	21.654181446387494	28.295347810225874	29.21147231441918	20.83899842896745
21	21.79273330842799	28.271861041378095	29.045983795506007	20.889421854687907
22	21.718002940979357	28.36086126985073	29.04390755366892	20.877228235501
23	21.778159467245523	28.387829471834188	28.930178826078528	20.903832234841765
24	21.666462053489813	28.381298705499752	28.912297859889556	21.039941381120876
25	21.72463601745615	28.438370474785753	28.895831935908824	20.941161571849282
26	21.72073876240558	28.430963109226067	28.831798228753502	21.016499899614853
27	21.759192252451964	28.41640073899297	28.69644389385162	21.127963114703444
28	21.707343561271518	28.481527054076377	28.753792905257956	21.057336479394145
29	21.797499487893806	28.450331807247785	28.700409974598447	21.051758730259966
30	21.72487690739305	28.409016315332043	28.72109783179843	21.145008945476484
31	21.807748781161443	28.396094290859743	28.61754096856739	21.178615959411424
32	21.814993831781802	28.479325012418737	28.57044165979413	21.135239496005326
33	21.793777164821226	28.41431876168119	28.543384148587563	21.248519924910017
34	21.789986931136507	28.47046405879004	28.541348849411204	21.19820016066225
35	21.79863526045647	28.411172789474758	28.511853352571272	21.278338597497502
36	21.78481835335841	28.41027238428873	28.5467910205523	21.25811824180056
37	21.84039797082347	28.403538937004903	28.515197158232887	21.240865933938746
38	21.843764694465385	28.406492706469272	28.510617381694438	21.239125217370905
39	21.801204604542317	28.438186939595727	28.469557118402307	21.291051337459642
40	21.870383032492867	28.471257686647327	28.406988825029796	21.251370455830003
41	21.812514276018437	28.352399191376488	28.52464354839838	21.31044298420669
42	21.866734062667728	28.417193482310388	28.35390792611156	21.362164528910327
43	21.837952680662394	28.449221152514585	28.400661747712423	21.312164419110594
44	21.810505821422613	28.359115160510463	28.418695290365093	21.41168372770183
45	21.86959153202269	28.32614175840925	28.43128457687859	21.37298213268947
46	21.764951243019507	28.380553671797237	28.482936247022288	21.371558838160965
47	21.834164723957254	28.432331805121812	28.377402576551376	21.35610089436956
48	21.775307062535834	28.364658686967946	28.442704871449358	21.417329379046855
49	21.856348952713763	28.385186232015556	28.36131805341654	21.397146761854135
50	21.787977477476314	28.33676319639778	28.400984044762843	21.474275281363056
51	21.850336182505583	28.313087502955074	28.349816795471245	21.486759519068098
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.21260084367117
2	39.86028058251057
3	39.3505660362532
4	39.846145513943426
5	43.38828960029104
6	41.563078997768315
7	43.88284237622207
8	41.25524648962423
9	40.661323746984465
10	40.929348214634196
11	43.991009410140016
12	41.042864729661126
13	43.10447823856231
14	42.76915329042209
15	43.062273748069906
16	43.12428570039767
17	43.14151310161615
18	42.96396771191582
19	42.58035622287676
20	42.49317987535494
21	42.6821551631159
22	42.59523117648036
23	42.68199170208729
24	42.70640343461069
25	42.66579758930543
26	42.73723866202043
27	42.88715536715541
28	42.76468004066567
29	42.84925821815377
30	42.86988585286953
31	42.98636474057286
32	42.95023332778713
33	43.04229708973124
34	42.98818709179876
35	43.07697385795397
36	43.04293659515896
37	43.08126390476221
38	43.08288991183629
39	43.09225594200196
40	43.121753488322874
41	43.12295726022513
42	43.22889859157805
43	43.15011709977299
44	43.22218954912444
45	43.24257366471216
46	43.136510081180475
47	43.19026561832681
48	43.192636441582685
49	43.253495714567904
50	43.26225275883937
51	43.33709570157368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28547.0
1	17066.5
2	5586.0
3	7086.5
4	8587.0
5	12561.5
6	16536.0
7	25363.5
8	34191.0
9	29090.0
10	23989.0
11	28635.5
12	33282.0
13	44312.0
14	55342.0
15	78304.0
16	101266.0
17	145362.5
18	189459.0
19	240397.0
20	291335.0
21	347816.0
22	404297.0
23	483688.0
24	563079.0
25	665650.0
26	893824.0
27	1019427.0
28	1115321.0
29	1211215.0
30	1304093.5
31	1396972.0
32	1505065.0
33	1613158.0
34	1718955.0
35	1824752.0
36	1970054.0
37	2115356.0
38	2201296.0
39	2287236.0
40	2364794.5
41	2442353.0
42	2470603.0
43	2498853.0
44	2501576.0
45	2504299.0
46	2452276.0
47	2400253.0
48	2318819.5
49	2237386.0
50	2125374.0
51	2013362.0
52	1856683.5
53	1700005.0
54	1547670.0
55	1395335.0
56	1242501.0
57	1089667.0
58	954561.0
59	819455.0
60	705290.5
61	591126.0
62	495249.5
63	399373.0
64	330869.0
65	262365.0
66	215160.5
67	167956.0
68	139628.5
69	111301.0
70	93585.0
71	75869.0
72	62953.0
73	50037.0
74	41665.5
75	27528.5
76	21763.0
77	19613.0
78	17463.0
79	16050.0
80	14637.0
81	12796.5
82	10956.0
83	9399.5
84	7843.0
85	6622.0
86	5401.0
87	5750.0
88	6099.0
89	3930.5
90	1762.0
91	1094.5
92	427.0
93	290.5
94	154.0
95	102.0
96	50.0
97	34.0
98	18.0
99	10.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022440044717201953
2	0.001918516283170365
3	0.005718268264038427
4	0.03044103190710527
5	0.0
6	0.0018238809508166701
7	0.0
8	0.0
9	0.0
10	0.0
11	4.301606016077051E-5
12	0.0
13	0.0
14	0.007461852569221659
15	0.0
16	0.0
17	0.005359801096032006
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	5.7354746881027355E-5
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	3.1545110784565045E-5
33	0.0
34	8.316438297748967E-5
35	4.014832281671915E-5
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.012999453380584851
42	5.190604592732976E-4
43	1.7206424064308206E-5
44	3.1545110784565045E-5
45	9.176759500964377E-5
46	0.007051766129022313
47	0.01151109769902219
48	0.00826195128821199
49	2.867737344051368E-6
50	0.005615029719652578
51	0.06839840339296917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4870697E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	23.76689586907113
#Duplication Level	Relative count
1	100.0
2	12.054761709664609
3	3.475553258409969
4	1.6381869638388664
5	0.9316045912096362
6	0.632835595111282
7	0.48912392610194705
8	0.3807098600071856
9	0.2893142810319506
10++	6.17077736667276
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	6682530	3.5089278	31.486591	4
TTTTT	10738835	3.1661985	3.8038151	18
TGTTT	5961660	2.3457108	23.49979	6
GTTTG	4231295	2.2218096	30.431524	7
CTTTG	4018840	2.2014697	31.7716	1
GAGGG	2183675	2.0956173	10.061972	11
GTGTT	3882240	2.0385246	30.401821	5
TTTGA	4788315	1.9334273	23.635399	8
TTTGT	4905785	1.9302598	23.118189	2
TTGTG	3628190	1.9051253	30.452665	3
TGAGG	2629960	1.8912406	12.376528	10
TTGAG	3156220	1.7007428	19.493843	9
AGGGG	1707420	1.6385676	5.9570775	12
GAGTG	1796955	1.2922153	5.22613	11
TGAGT	2343105	1.2625923	6.1819854	10
TTGAT	2366545	0.9555643	5.3745365	9
>>END_MODULE