FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_10_GATCAG_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_10_GATCAG_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 37014713
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11819620 3.7028396 4.642635 16
TGTGT 7176455 3.4469202 37.388275 4
AAAAA 7833525 3.0848055 3.2427227 10
TGTTT 7064245 2.740262 30.478897 6
GTTTG 5697230 2.7364345 37.07247 7
GGGGG 2867975 2.6150594 5.038648 13
TTTGA 6283400 2.5514607 31.585232 8
GTGTT 5271530 2.5319664 36.947796 5
CTTTG 4809095 2.445542 39.377193 1
TTGTG 4795765 2.303452 36.836697 3
GAGGG 2836645 2.186674 10.527668 11
TTTGT 5434880 2.1082218 30.138391 2
TTGAG 3909015 1.9654248 23.115698 9
TGAGG 2921050 1.8185412 13.540157 10
AGGGG 2002760 1.5438602 5.6606097 12
AACTT 3417995 1.5382391 11.004909 2
ACTTT 3507295 1.5078458 10.2039385 3
TGAGC 2125870 1.401236 5.7431693 10
TGAGT 2334195 1.1736169 6.51557 10
TTGAT 2824390 1.1468823 6.710094 9
TAACT 2398440 1.0793973 10.697179 1
TTGAC 1936685 1.0309523 6.916185 9