##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_10_GATCAG_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	37014713	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13150130327905	33.0	31.0	34.0	30.0	34.0
2	32.3060714262461	34.0	31.0	34.0	31.0	34.0
3	32.36848449966369	34.0	31.0	34.0	31.0	34.0
4	35.828747368647704	37.0	35.0	37.0	35.0	37.0
5	35.8513760190441	37.0	35.0	37.0	35.0	37.0
6	35.83605492226834	37.0	36.0	37.0	35.0	37.0
7	35.881162012521884	37.0	37.0	37.0	35.0	37.0
8	35.88454166860621	37.0	36.0	37.0	35.0	37.0
9	37.615105134004416	39.0	38.0	39.0	35.0	39.0
10	37.6070101637692	39.0	38.0	39.0	35.0	39.0
11	37.565016997430185	39.0	38.0	39.0	35.0	39.0
12	37.537044472018465	39.0	38.0	39.0	35.0	39.0
13	37.530671681825545	39.0	38.0	39.0	35.0	39.0
14	38.976771047772274	41.0	39.0	41.0	36.0	41.0
15	38.93620915012903	41.0	39.0	41.0	36.0	41.0
16	38.88810568381281	41.0	39.0	41.0	36.0	41.0
17	38.87658180680747	41.0	39.0	41.0	36.0	41.0
18	38.81143357777757	41.0	39.0	41.0	35.0	41.0
19	38.82212116571051	41.0	39.0	41.0	35.0	41.0
20	38.80289321708371	40.0	39.0	41.0	35.0	41.0
21	38.76369801921739	40.0	39.0	41.0	35.0	41.0
22	38.68683026125314	40.0	39.0	41.0	35.0	41.0
23	38.6572907913672	40.0	39.0	41.0	35.0	41.0
24	38.60554120735719	40.0	39.0	41.0	34.0	41.0
25	38.56002106513699	40.0	39.0	41.0	34.0	41.0
26	38.49600257605672	40.0	38.0	41.0	34.0	41.0
27	38.44585829964425	40.0	38.0	41.0	34.0	41.0
28	38.37006216960267	40.0	38.0	41.0	34.0	41.0
29	38.30145018279623	40.0	38.0	41.0	34.0	41.0
30	38.25458130662799	40.0	38.0	41.0	34.0	41.0
31	38.19443700671136	40.0	38.0	41.0	33.0	41.0
32	38.12213891811075	40.0	38.0	41.0	33.0	41.0
33	38.059167499150945	40.0	38.0	41.0	33.0	41.0
34	37.99903530252956	40.0	38.0	41.0	33.0	41.0
35	37.93739208514193	40.0	38.0	41.0	33.0	41.0
36	37.858174531840895	40.0	38.0	41.0	33.0	41.0
37	37.77769253539802	40.0	38.0	41.0	33.0	41.0
38	37.6929912437792	40.0	38.0	41.0	32.0	41.0
39	37.62259764110558	40.0	37.0	41.0	32.0	41.0
40	37.50811086931837	40.0	37.0	41.0	32.0	41.0
41	37.7080737867669	40.0	38.0	41.0	33.0	41.0
42	37.74371304729554	40.0	38.0	41.0	33.0	41.0
43	37.72256696951831	40.0	38.0	41.0	33.0	41.0
44	37.672836312414475	40.0	37.0	41.0	33.0	41.0
45	37.60924065519568	40.0	37.0	41.0	32.0	41.0
46	37.55026397205889	40.0	37.0	41.0	32.0	41.0
47	37.481758402395286	40.0	37.0	41.0	32.0	41.0
48	37.40183021275891	40.0	37.0	41.0	32.0	41.0
49	37.32451101268839	40.0	37.0	41.0	31.0	41.0
50	37.25037357442161	40.0	36.0	41.0	31.0	41.0
51	35.71209567395538	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	20.0
6	382.0
7	2407.0
8	8050.0
9	12985.0
10	16554.0
11	21950.0
12	31065.0
13	42521.0
14	52628.0
15	59069.0
16	63521.0
17	65895.0
18	68342.0
19	71164.0
20	74865.0
21	80761.0
22	89138.0
23	99280.0
24	113044.0
25	129979.0
26	149825.0
27	176095.0
28	209665.0
29	253284.0
30	311361.0
31	390129.0
32	504575.0
33	673521.0
34	958346.0
35	1411666.0
36	2131847.0
37	3447670.0
38	7457724.0
39	1.7832381E7
40	2996.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.908541482082946	26.640010514771266	29.326556186823062	24.124891816322723
2	21.93178155940315	28.049019318345113	31.271019175537035	18.748179946714703
3	22.43088309235303	26.994854721688643	30.605421687316607	19.968840498641715
4	21.765120804799974	25.273231214841513	31.78175662202217	21.179891358336345
5	26.937891188564834	25.46179402208772	27.92067446770555	19.679640321641898
6	21.476247566983535	25.15973264403225	33.043457070139716	20.3205627188445
7	25.455245071137483	25.562896307937667	29.09148204048979	19.89037658043506
8	22.247749333106967	25.676091627105528	31.83620101547512	20.239958024312386
9	22.439702546205453	25.876713137461536	31.47330193192044	20.210282384412572
10	22.18565865152047	27.19071019154786	30.511366606720014	20.11226455021166
11	26.093744503333138	25.724454950851726	27.767441229604138	20.414359316210994
12	21.51183693235313	29.91628590237085	28.01561809206011	20.55625907321592
13	24.11935474693694	26.055455979132024	28.698192135177557	21.126997138753474
14	24.362566156900435	26.389887278287084	27.906447003287276	21.34109956152521
15	23.605393859179234	27.410423645508665	27.460497577035582	21.523684918276523
16	23.776478096140686	26.64598179488417	27.844474353481324	21.733065755493822
17	23.63017320050721	26.698361852822934	27.978957459787136	21.692507486882725
18	23.387765304392282	26.728808635889568	28.07270092220634	21.810725137511806
19	23.024188786734257	27.034928669655912	28.40994660594136	21.53093593766847
20	22.735294780375902	27.07406546642775	28.428215342705492	21.76242441049085
21	22.880499162850416	27.135133987295124	28.257176221088432	21.727190628766028
22	22.632870994960914	27.266845873172326	28.482409140747816	21.61787399111894
23	22.637173372770356	27.416322182594882	28.273480323410226	21.673024121224532
24	22.509298019335816	27.44335722452224	28.229803376706165	21.817541379435777
25	22.524615850725844	27.650418311276155	28.07918527549495	21.74578056250305
26	22.562228410196656	27.539363478926575	28.07371811450513	21.824689996371642
27	22.558144185635488	27.487838236999057	28.002152242220756	21.951865335144703
28	22.553435689700514	27.592789107169864	28.00036138109176	21.853413822037858
29	22.6075457573969	27.57418709934415	27.910683081221705	21.907584062037248
30	22.635343122539755	27.534823853155043	27.81003265551395	22.019800368791252
31	22.74261480943179	27.47111010895332	27.750343618184797	22.035931463430096
32	22.770716035216733	27.564140442724884	27.629223917658273	22.035919604400117
33	22.764279220754307	27.469727610504037	27.61457315659266	22.151420012148996
34	22.799746050809617	27.507011947209133	27.575846595925995	22.117395406055255
35	22.79662788662609	27.469239391661187	27.512573116710282	22.221559605002437
36	22.87808453496732	27.480135912575314	27.515468340026572	22.12631121243079
37	22.86092817521666	27.398980168342696	27.549068427578298	22.191023228862342
38	22.81338638805757	27.466894554181494	27.637121057936547	22.082597999824394
39	22.780670137859055	27.347008161034736	27.62082069070474	22.251501010401473
40	22.846848108883165	27.414441794321547	27.583661072223997	22.15504902457129
41	22.829984883197433	27.379999232539877	27.55777164169989	22.2322442425628
42	22.753114423761044	27.34008205873206	27.606478545083757	22.30032497242313
43	22.799630116134562	27.46129906304915	27.459256162681882	22.279814658134406
44	22.90916714091107	27.325318042305057	27.46804662668829	22.297468190095582
45	22.8824792777601	27.316152909153185	27.46798110761377	22.33338670547294
46	22.744384464462037	27.456536494663453	27.408906921982584	22.390172118891932
47	22.85014816557016	27.37842597752743	27.421031145034075	22.350394711868336
48	22.79533207685757	27.290876301571913	27.387698559550323	22.52609306202019
49	22.849834619863476	27.414774949661773	27.37901430709432	22.356376123380432
50	22.74602183829355	27.386633545292614	27.449230650660212	22.418113965753623
51	22.837083659980443	27.34588334316486	27.395677843832406	22.42135515302229
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.03343329840567
2	40.67996150611785
3	42.399723590994746
4	42.94501216313632
5	46.617531510206724
6	41.79681028582804
7	45.34562165157254
8	42.48770735741935
9	42.649984930618025
10	42.297923201732125
11	46.50810381954413
12	42.06809600556905
13	45.246351885690416
14	45.70366571842564
15	45.12907877745575
16	45.509543851634504
17	45.32268068738993
18	45.19849044190409
19	44.555124724402724
20	44.49771919086676
21	44.607689791616444
22	44.25074498607985
23	44.310197493994885
24	44.3268393987716
25	44.27039641322889
26	44.3869184065683
27	44.510009520780194
28	44.40684951173837
29	44.515129819434144
30	44.655143491331
31	44.77854627286188
32	44.80663563961685
33	44.91569923290331
34	44.917141456864876
35	45.01818749162853
36	45.004395747398114
37	45.051951404079006
38	44.89598438788196
39	45.032171148260524
40	45.001897133454456
41	45.06222912576023
42	45.053439396184174
43	45.079444774268964
44	45.20663533100665
45	45.21586598323304
46	45.13455658335397
47	45.200542877438494
48	45.321425138877764
49	45.20621074324391
50	45.16413580404717
51	45.25843881300273
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14723.0
1	9553.0
2	4383.0
3	5934.5
4	7486.0
5	20784.5
6	34083.0
7	42868.0
8	51653.0
9	39168.0
10	26683.0
11	27958.5
12	29234.0
13	37710.5
14	46187.0
15	68149.0
16	90111.0
17	137506.0
18	184901.0
19	233721.5
20	282542.0
21	335374.0
22	388206.0
23	459655.5
24	531105.0
25	632255.5
26	869136.0
27	1004866.0
28	1084454.0
29	1164042.0
30	1233978.5
31	1303915.0
32	1405211.5
33	1506508.0
34	1600936.5
35	1695365.0
36	1886092.5
37	2076820.0
38	2170806.0
39	2264792.0
40	2376618.0
41	2488444.0
42	2484699.0
43	2480954.0
44	2472322.5
45	2463691.0
46	2414262.5
47	2364834.0
48	2317125.0
49	2269416.0
50	2179125.5
51	2088835.0
52	1949588.5
53	1810342.0
54	1687523.0
55	1564704.0
56	1436099.0
57	1307494.0
58	1176476.5
59	1045459.0
60	939419.5
61	833380.0
62	728573.5
63	623767.0
64	542010.5
65	460254.0
66	392151.0
67	324048.0
68	310000.5
69	295953.0
70	273697.5
71	251442.0
72	220216.5
73	188991.0
74	162878.0
75	116049.5
76	95334.0
77	92752.0
78	90170.0
79	91130.5
80	92091.0
81	81447.5
82	70804.0
83	66052.0
84	61300.0
85	56137.0
86	50974.0
87	61252.0
88	71530.0
89	48880.0
90	26230.0
91	17343.0
92	8456.0
93	6408.5
94	4361.0
95	3323.5
96	2286.0
97	1703.5
98	1121.0
99	696.5
100	272.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.187921516506153E-4
2	0.0
3	0.0
4	0.0
5	0.002037027816479355
6	0.002671910491376767
7	0.02709463126189848
8	0.0024476753338598086
9	0.006964798025044797
10	0.003371632247965829
11	0.009852838788727065
12	0.027537698320124756
13	0.004927770208565442
14	0.00627588278207101
15	0.009290900080732762
16	0.03281668022118664
17	0.003541834837406412
18	0.001180611612468804
19	0.02356630456651116
20	0.005324909583926803
21	0.0011346839296038848
22	0.024282236093523136
23	0.0011427888148153411
24	0.031530705100968906
25	0.0036931260280202633
26	0.03176844840050496
27	0.030914733824898222
28	0.003120380806410683
29	0.03775255531496354
30	0.003679617886001169
31	0.0033689306195620102
32	0.010217558623242601
33	0.01149542885824888
34	0.015493838895900666
35	0.01779292466755044
36	0.016796023786541315
37	0.021129435746266627
38	0.031506390445334535
39	0.020643142633579248
40	0.023995863482718344
41	0.02575462357360437
42	0.01713372733701866
43	0.022971946317671032
44	0.008661420662642987
45	0.028064515858869418
46	0.03941946004011972
47	0.020167656034507143
48	0.043196336548658364
49	0.0203918911920241
50	0.02649486975625071
51	0.09924161778587881
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.7014713E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	38.917746127902724
#Duplication Level	Relative count
1	100.0
2	13.657400894690937
3	5.005908336381284
4	2.608811861688659
5	1.5657091410404298
6	1.1380581267760177
7	0.8363109473032665
8	0.6414765213966181
9	0.4951748586219509
10++	14.725825056973244
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11819620	3.7028396	4.642635	16
TGTGT	7176455	3.4469202	37.388275	4
AAAAA	7833525	3.0848055	3.2427227	10
TGTTT	7064245	2.740262	30.478897	6
GTTTG	5697230	2.7364345	37.07247	7
GGGGG	2867975	2.6150594	5.038648	13
TTTGA	6283400	2.5514607	31.585232	8
GTGTT	5271530	2.5319664	36.947796	5
CTTTG	4809095	2.445542	39.377193	1
TTGTG	4795765	2.303452	36.836697	3
GAGGG	2836645	2.186674	10.527668	11
TTTGT	5434880	2.1082218	30.138391	2
TTGAG	3909015	1.9654248	23.115698	9
TGAGG	2921050	1.8185412	13.540157	10
AGGGG	2002760	1.5438602	5.6606097	12
AACTT	3417995	1.5382391	11.004909	2
ACTTT	3507295	1.5078458	10.2039385	3
TGAGC	2125870	1.401236	5.7431693	10
TGAGT	2334195	1.1736169	6.51557	10
TTGAT	2824390	1.1468823	6.710094	9
TAACT	2398440	1.0793973	10.697179	1
TTGAC	1936685	1.0309523	6.916185	9
>>END_MODULE