FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_D_10_GATCAG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_10_GATCAG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 37014713
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11113525 3.5197458 4.3323817 16
TGTGT 5896710 2.934557 41.28211 4
TGTTT 5977980 2.3732889 33.059193 6
GTTTG 4569845 2.274229 40.711758 7
CTTTG 4402515 2.2683182 42.399094 1
TTTGA 5223035 2.1480525 33.919838 8
GAGGG 2567440 2.0798507 11.642477 11
GTGTT 4150765 2.0656698 40.684967 5
TTTGT 5026810 1.9956691 32.942593 2
TTGTG 3781080 1.8816922 40.71783 3
TGAGG 2632120 1.7009866 15.100068 10
TTGAG 3242545 1.6716471 24.99645 9
AGGGG 1787960 1.4484037 6.188933 12
TGAGC 2019060 1.3508741 5.9894476 10
TGAGT 2178820 1.1232591 6.9312897 10
TTGAT 2623525 1.0789646 7.2772975 9
TTGAC 1809145 0.9656113 7.4587398 9
Produced by FastQC (version 0.10.1)