##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_10_GATCAG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	37014713	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.579110366194115	34.0	31.0	34.0	31.0	34.0
2	32.853997922393724	34.0	31.0	34.0	31.0	34.0
3	32.95015895435958	34.0	31.0	34.0	31.0	34.0
4	36.39537850800032	37.0	37.0	37.0	35.0	37.0
5	36.30662301771731	37.0	37.0	37.0	35.0	37.0
6	36.27010281019874	37.0	37.0	37.0	35.0	37.0
7	36.27991906893888	37.0	37.0	37.0	35.0	37.0
8	36.247080559560196	37.0	37.0	37.0	35.0	37.0
9	38.00255855016355	39.0	38.0	39.0	35.0	39.0
10	37.99141868261953	39.0	38.0	39.0	35.0	39.0
11	37.98561955620188	39.0	38.0	39.0	35.0	39.0
12	37.950093628984774	39.0	38.0	39.0	35.0	39.0
13	37.95066453709907	39.0	38.0	39.0	35.0	39.0
14	39.44411955861984	41.0	39.0	41.0	37.0	41.0
15	39.44631695509837	41.0	39.0	41.0	37.0	41.0
16	39.431137180504415	41.0	39.0	41.0	37.0	41.0
17	39.4038477618346	41.0	39.0	41.0	36.0	41.0
18	39.354410069314866	41.0	39.0	41.0	36.0	41.0
19	39.35943842114891	41.0	39.0	41.0	36.0	41.0
20	39.32839587328423	41.0	39.0	41.0	36.0	41.0
21	39.30187339288569	40.0	39.0	41.0	36.0	41.0
22	39.27627000647013	40.0	39.0	41.0	36.0	41.0
23	39.25613295988544	40.0	39.0	41.0	36.0	41.0
24	39.21300735196839	40.0	39.0	41.0	36.0	41.0
25	39.160821590052585	40.0	39.0	41.0	36.0	41.0
26	39.08593831323236	40.0	39.0	41.0	36.0	41.0
27	38.99935674227705	40.0	39.0	41.0	35.0	41.0
28	38.95127278171791	40.0	39.0	41.0	35.0	41.0
29	38.865590231646536	40.0	38.0	41.0	35.0	41.0
30	38.772327641713716	40.0	38.0	41.0	35.0	41.0
31	38.71226285072101	40.0	38.0	41.0	35.0	41.0
32	38.64218401477272	40.0	38.0	41.0	35.0	41.0
33	38.556439975638874	40.0	38.0	41.0	34.0	41.0
34	38.700536297552816	40.0	38.0	41.0	35.0	41.0
35	38.71611548088999	40.0	38.0	41.0	35.0	41.0
36	38.691711212241465	40.0	38.0	41.0	35.0	41.0
37	38.65912203614817	40.0	38.0	41.0	35.0	41.0
38	38.61162578783199	40.0	38.0	41.0	35.0	41.0
39	38.521609339507776	40.0	38.0	41.0	35.0	41.0
40	38.463412670523745	40.0	38.0	41.0	34.0	41.0
41	38.41814567088498	40.0	38.0	41.0	34.0	41.0
42	37.771905025982505	40.0	38.0	41.0	33.0	41.0
43	38.0042505260003	40.0	38.0	41.0	33.0	41.0
44	38.104819886081515	40.0	38.0	41.0	33.0	41.0
45	38.10115147995339	40.0	38.0	41.0	33.0	41.0
46	38.05519899613972	40.0	38.0	41.0	33.0	41.0
47	37.982425772151736	40.0	37.0	41.0	33.0	41.0
48	37.91273243156039	40.0	37.0	41.0	33.0	41.0
49	37.73363756731006	40.0	37.0	41.0	33.0	41.0
50	37.8831774813437	40.0	37.0	41.0	33.0	41.0
51	37.6556403935916	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	15.0
11	26.0
12	74.0
13	115.0
14	248.0
15	373.0
16	689.0
17	1228.0
18	2150.0
19	3990.0
20	7310.0
21	12721.0
22	21362.0
23	33252.0
24	49692.0
25	70848.0
26	97291.0
27	130907.0
28	173133.0
29	227152.0
30	295563.0
31	381089.0
32	494590.0
33	659188.0
34	941077.0
35	1403626.0
36	2129353.0
37	3501796.0
38	7522393.0
39	1.8843872E7
40	9582.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.040458582905814	27.768592928573206	28.021100840996738	25.169847647524247
2	20.733429511598125	27.460250819946726	31.89435790201704	19.911961766438107
3	19.882461394144467	26.382275511097326	32.87771002893075	20.857553065827457
4	20.83395385200604	26.189267393717248	32.47183719132428	20.504941562952432
5	26.201383757858665	26.31214782078683	27.637010180249135	19.84945824110537
6	21.93201449196741	26.08596819556505	31.305336105276194	20.676681207191347
7	26.035857687185093	26.586803469204263	27.02572082620227	20.351618017408374
8	21.468684628190957	26.533532760337764	31.218731859409527	20.779050752061753
9	21.059301472903492	26.611744902628313	31.55695952579721	20.77199409867098
10	21.33342219889696	26.606201161143677	31.39874676321278	20.66162987674658
11	25.683356421574672	26.44723124421156	27.10032141436478	20.76909091984899
12	21.280937663895976	30.900196362457276	26.961349126224484	20.857516847422268
13	23.884011308800673	26.814910784663137	27.857434092692657	21.443643813843533
14	23.14816813519532	27.033504217633674	28.23749302068072	21.580834626490283
15	23.309512085099783	26.942326960633196	27.9840667682605	21.76409418600652
16	23.069901960336693	26.97060220350756	28.07570060046123	21.88379523569452
17	23.13412362142105	27.041057150859295	27.93185368799877	21.89296553972089
18	22.905361711945922	27.079342346091927	28.106549345994804	21.908746595967347
19	22.558453985581355	27.419215704846884	28.421760287591585	21.60057002198018
20	22.313073182547708	27.424586542113673	28.449108871923446	21.813231403415177
21	22.511129560831662	27.375638438693283	28.23772265909505	21.875509341380006
22	22.386606104442848	27.429546731863084	28.398626243569687	21.785220920124384
23	22.51144835298331	27.491946243106085	28.1692093627742	21.8273960411364
24	22.321064599366203	27.53477785982023	28.184230416699435	21.95992712411413
25	22.371941665466917	27.766021041416693	28.00787621938336	21.85416107373303
26	22.425939112374042	27.631434019223654	27.973957274773415	21.96866959362889
27	22.476018698260066	27.56841921182004	27.884686588391	22.070875501528896
28	22.436983369288853	27.65068312160086	27.964004475733745	21.948329033376538
29	22.510421734189862	27.637069616074015	27.86987433888789	21.98263431084823
30	22.53015172642295	27.584377055685938	27.8314652878708	22.054005930020317
31	22.63559464098602	27.513137029951253	27.747968405478424	22.1032999235843
32	22.642308762260612	27.638579284821606	27.617841504516775	22.101270448401007
33	22.656011622662657	27.544587697897004	27.60770606567974	22.1916946137606
34	22.623697258847066	27.58728680464953	27.631785409039733	22.15723052746367
35	22.633436245430396	27.585863321384572	27.535977380933822	22.244723052251206
36	22.780534311393733	27.582913963704424	27.489828778643215	22.14672294625863
37	22.746360345952162	27.45257811400564	27.557952428268184	22.24310911177401
38	22.67666888857616	27.52399053035916	27.672963739901345	22.126376841163335
39	22.64678912950102	27.439064568729737	27.648886538712325	22.26525976305692
40	22.68792952683437	27.500880528237516	27.609880427817984	22.20130951711013
41	22.724360567654255	27.393726927698864	27.60062116902541	22.28129133562147
42	22.77046969857021	27.531911065339372	27.49062734451469	22.20699189157573
43	22.66560143052583	27.56141549501993	27.474852450953126	22.298130623501116
44	22.82346482059715	27.35369040954066	27.51483714057164	22.308007629290547
45	22.802754137253476	27.283548031292316	27.526154261955238	22.387543569498973
46	22.638568074268196	27.488628643426193	27.5024258596845	22.370377422621107
47	22.733132586972445	27.49607960464723	27.448443974341334	22.32234383403899
48	22.68491221426249	27.385573276134362	27.4400930072352	22.489421502367954
49	22.8831383887807	27.488226100794027	27.38176843354155	22.246867076883724
50	22.632429134056878	27.416565428350548	27.48328395778467	22.467721479807903
51	22.737636771328564	27.386183617515293	27.42396620042563	22.45221341073051
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.210306230430064
2	40.645391278036236
3	40.740014459971924
4	41.33889541495847
5	46.05084199896403
6	42.60869569915876
7	46.38747570459347
8	42.24773538025271
9	41.83129557157447
10	41.99505207564354
11	46.45244734142366
12	42.138454511318244
13	45.3276551226442
14	44.72900276168561
15	45.0736062711063
16	44.953697196031214
17	45.027089161141944
18	44.81410830791327
19	44.159024007561534
20	44.126304585962885
21	44.386638902211665
22	44.17182702456723
23	44.33884439411971
24	44.280991723480334
25	44.22610273919995
26	44.394608706002934
27	44.546894199788966
28	44.38531240266539
29	44.493056045038095
30	44.58415765644326
31	44.738894564570316
32	44.743579210661615
33	44.84770623642326
34	44.780927786310734
35	44.878159297681606
36	44.927257257652364
37	44.98946945772617
38	44.803045729739495
39	44.91204889255794
40	44.8892390439445
41	45.00565190327573
42	44.97746159014594
43	44.96373205402694
44	45.1314724498877
45	45.190297706752446
46	45.008945496889304
47	45.055476421011434
48	45.17433371663044
49	45.130005465664425
50	45.100150613864784
51	45.189850182059075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24043.0
1	14649.5
2	5256.0
3	6717.5
4	8179.0
5	12825.5
6	17472.0
7	32103.0
8	46734.0
9	35580.5
10	24427.0
11	26138.5
12	27850.0
13	37799.0
14	47748.0
15	72416.0
16	97084.0
17	149171.0
18	201258.0
19	255028.0
20	308798.0
21	361717.5
22	414637.0
23	489316.0
24	563995.0
25	673202.5
26	925042.0
27	1067674.0
28	1136179.0
29	1204684.0
30	1264889.0
31	1325094.0
32	1422422.0
33	1519750.0
34	1608336.0
35	1696922.0
36	1881141.0
37	2065360.0
38	2151376.5
39	2237393.0
40	2339535.5
41	2441678.0
42	2436693.0
43	2431708.0
44	2422234.0
45	2412760.0
46	2364617.5
47	2316475.0
48	2277628.0
49	2238781.0
50	2154210.0
51	2069639.0
52	1938595.0
53	1807551.0
54	1688626.5
55	1569702.0
56	1440606.5
57	1311511.0
58	1179766.5
59	1048022.0
60	943148.0
61	838274.0
62	730841.5
63	623409.0
64	542387.5
65	461366.0
66	393383.0
67	325400.0
68	313462.0
69	301524.0
70	277322.0
71	253120.0
72	222423.5
73	191727.0
74	164743.0
75	115058.5
76	92358.0
77	91678.5
78	90999.0
79	92010.5
80	93022.0
81	81716.0
82	70410.0
83	64144.5
84	57879.0
85	53617.0
86	49355.0
87	59358.0
88	69361.0
89	44192.5
90	19024.0
91	11282.0
92	3540.0
93	2413.5
94	1287.0
95	753.5
96	220.0
97	147.0
98	74.0
99	42.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026832573306728058
2	3.052840096315214E-4
3	0.00592467108957457
4	0.04348270915946315
5	0.0
6	2.7016284038187733E-6
7	0.0
8	0.0
9	0.0
10	0.0
11	0.006392052803435217
12	0.0
13	2.7016284038187733E-6
14	0.0
15	0.0
16	0.0
17	0.008728961372738457
18	0.004398251041416963
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	3.9443774695754094E-4
28	0.0
29	0.0
30	0.0
31	1.837107314596766E-4
32	3.890344901499034E-4
33	3.539133209002593E-4
34	1.8641235986349537E-4
35	7.456494394539815E-4
36	6.213745328783179E-4
37	0.0
38	1.0806513615275093E-5
39	0.0
40	0.0
41	0.0017101307796172835
42	3.3230029366970916E-4
43	1.945172450749517E-4
44	0.0
45	0.0
46	0.0
47	0.006759474266354571
48	0.011506235371864156
49	0.0
50	0.014869762734618529
51	0.03657194370249473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.7014713E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	40.62333645973282
#Duplication Level	Relative count
1	100.0
2	14.39081833770928
3	5.179271529304616
4	2.7500871631765818
5	1.70697518873496
6	1.173323087212984
7	0.823247308614568
8	0.6809400815420411
9	0.5834596309973602
10++	15.019104745234486
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11113525	3.5197458	4.3323817	16
TGTGT	5896710	2.934557	41.28211	4
TGTTT	5977980	2.3732889	33.059193	6
GTTTG	4569845	2.274229	40.711758	7
CTTTG	4402515	2.2683182	42.399094	1
TTTGA	5223035	2.1480525	33.919838	8
GAGGG	2567440	2.0798507	11.642477	11
GTGTT	4150765	2.0656698	40.684967	5
TTTGT	5026810	1.9956691	32.942593	2
TTGTG	3781080	1.8816922	40.71783	3
TGAGG	2632120	1.7009866	15.100068	10
TTGAG	3242545	1.6716471	24.99645	9
AGGGG	1787960	1.4484037	6.188933	12
TGAGC	2019060	1.3508741	5.9894476	10
TGAGT	2178820	1.1232591	6.9312897	10
TTGAT	2623525	1.0789646	7.2772975	9
TTGAC	1809145	0.9656113	7.4587398	9
>>END_MODULE