FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 140701_JD_C_7_ACTTGA_L004_R2_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 31548134 |
| Filtered Sequences | 0 |
| Sequence length | 51 |
| %GC | 44 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
![[FAIL]](Icons/error.png)
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TGTGT | 8535325 | 4.517308 | 44.980816 | 4 |
| TTTTT | 12483040 | 4.266809 | 5.6151924 | 18 |
| AAAAA | 7289625 | 3.4994378 | 3.7042077 | 44 |
| GTTTG | 6275900 | 3.321511 | 44.453995 | 7 |
| TGTTT | 7614975 | 3.2388418 | 36.23447 | 6 |
| GTGTT | 5987930 | 3.169103 | 44.363495 | 5 |
| TTTGA | 6727750 | 3.0626202 | 38.39773 | 8 |
| CTTTG | 5084185 | 2.934528 | 48.59038 | 1 |
| TTGTG | 5523590 | 2.923352 | 44.43561 | 3 |
| GGGGG | 2621625 | 2.6732862 | 5.709844 | 13 |
| TTTGT | 5831065 | 2.4800997 | 35.963444 | 2 |
| TTGAG | 4161115 | 2.3570642 | 27.958176 | 9 |
| GAGGG | 2659780 | 2.3328362 | 12.28371 | 11 |
| TGAGG | 2980220 | 2.1006243 | 16.267765 | 10 |
| AGGGG | 1940440 | 1.7019184 | 6.7811275 | 12 |
| ACTTT | 3401845 | 1.6888654 | 17.55497 | 3 |
| AACTT | 3042320 | 1.6165439 | 19.299944 | 2 |
| TGAGC | 1945715 | 1.4956727 | 6.339103 | 10 |
| TGAGA | 2453710 | 1.4876027 | 5.3385167 | 10 |
| TGAGT | 2355920 | 1.334511 | 8.0196295 | 10 |
| GAGTG | 1861795 | 1.3122964 | 6.2485805 | 11 |
| TAACT | 2395895 | 1.2730643 | 19.164312 | 1 |
| TTGAT | 2648700 | 1.2057467 | 8.603378 | 9 |
| TTGAC | 1732400 | 1.0702053 | 7.9317846 | 9 |
| GTAAC | 1124160 | 0.74327433 | 5.8721304 | 1 |