##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_7_ACTTGA_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	31548134	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.194062856459276	33.0	31.0	34.0	31.0	34.0
2	32.35561650017082	34.0	31.0	34.0	31.0	34.0
3	32.44495084875702	34.0	31.0	34.0	31.0	34.0
4	35.90568523006781	37.0	35.0	37.0	35.0	37.0
5	35.933244926625456	37.0	37.0	37.0	35.0	37.0
6	35.90967278762034	37.0	36.0	37.0	35.0	37.0
7	35.95862544516896	37.0	37.0	37.0	35.0	37.0
8	35.9410826009551	37.0	37.0	37.0	35.0	37.0
9	37.65930767886304	39.0	38.0	39.0	35.0	39.0
10	37.65542431130792	39.0	38.0	39.0	35.0	39.0
11	37.64297517564747	39.0	38.0	39.0	35.0	39.0
12	37.58102162238819	39.0	38.0	39.0	35.0	39.0
13	37.564436616124425	39.0	38.0	39.0	35.0	39.0
14	39.038388070749285	41.0	39.0	41.0	36.0	41.0
15	38.991286933166954	41.0	39.0	41.0	36.0	41.0
16	38.95432341576843	41.0	39.0	41.0	36.0	41.0
17	38.93209493784957	41.0	39.0	41.0	36.0	41.0
18	38.89583799155918	41.0	39.0	41.0	36.0	41.0
19	38.89305627394634	41.0	39.0	41.0	35.0	41.0
20	38.8644230749115	41.0	39.0	41.0	35.0	41.0
21	38.817040430980796	41.0	39.0	41.0	35.0	41.0
22	38.77273901524572	40.0	39.0	41.0	35.0	41.0
23	38.74205834170731	40.0	39.0	41.0	35.0	41.0
24	38.695305180331744	40.0	39.0	41.0	35.0	41.0
25	38.621112773262595	40.0	39.0	41.0	35.0	41.0
26	38.542097608689	40.0	38.0	41.0	34.0	41.0
27	38.50564686329784	40.0	38.0	41.0	34.0	41.0
28	38.462404939702616	40.0	38.0	41.0	34.0	41.0
29	38.375431776725684	40.0	38.0	41.0	34.0	41.0
30	38.32687144032037	40.0	38.0	41.0	34.0	41.0
31	38.261657567449156	40.0	38.0	41.0	34.0	41.0
32	38.204705038973145	40.0	38.0	41.0	34.0	41.0
33	38.128863881458095	40.0	38.0	41.0	33.0	41.0
34	38.068135947438286	40.0	38.0	41.0	33.0	41.0
35	38.00526668233373	40.0	38.0	41.0	33.0	41.0
36	37.9425741313258	40.0	38.0	41.0	33.0	41.0
37	37.87060632492559	40.0	38.0	41.0	33.0	41.0
38	37.80691647246078	40.0	38.0	41.0	33.0	41.0
39	37.709922431545394	40.0	38.0	41.0	33.0	41.0
40	37.62540212362481	40.0	38.0	41.0	32.0	41.0
41	37.81207693615096	40.0	38.0	41.0	33.0	41.0
42	37.84944275943547	40.0	38.0	41.0	33.0	41.0
43	37.82725003640469	40.0	38.0	41.0	33.0	41.0
44	37.788397215505675	40.0	38.0	41.0	33.0	41.0
45	37.717729295811914	40.0	38.0	41.0	33.0	41.0
46	37.66673455235102	40.0	37.0	41.0	33.0	41.0
47	37.60935997038684	40.0	37.0	41.0	32.0	41.0
48	37.541233468832104	40.0	37.0	41.0	32.0	41.0
49	37.467661352015305	40.0	37.0	41.0	32.0	41.0
50	37.381175729759484	40.0	37.0	41.0	32.0	41.0
51	35.8818484161377	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	9.0
6	313.0
7	2432.0
8	8149.0
9	12201.0
10	13309.0
11	16958.0
12	24400.0
13	34152.0
14	42896.0
15	48196.0
16	51528.0
17	54088.0
18	55735.0
19	58091.0
20	61389.0
21	65741.0
22	72844.0
23	81089.0
24	92472.0
25	106298.0
26	122881.0
27	143429.0
28	170639.0
29	205669.0
30	252545.0
31	314609.0
32	405549.0
33	543175.0
34	778031.0
35	1160881.0
36	1753667.0
37	2837104.0
38	6264323.0
39	1.5690755E7
40	2586.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.30236869754775	25.869427243107562	30.71651833706483	24.11168572227986
2	21.273770378935126	28.09152973990964	32.544496414813736	18.0902034663415
3	22.402573794063382	27.15248388383288	31.477300685993033	18.967641636110713
4	21.26684445409235	24.436542264497174	33.08583247575753	21.210780805652952
5	27.91379613262607	24.3723406407006	28.729148584656084	18.984714642017245
6	20.784996928588235	24.010518182573794	35.78032720346453	19.42415768537344
7	25.954043187630642	24.42974720072157	30.761122628935706	18.855086982712084
8	22.919509732597266	24.646946289630556	33.28792669763727	19.145617280134914
9	22.779169812124657	25.048474105873346	33.01378660855808	19.15856947344391
10	22.78967200011285	26.75128095113485	31.308599740156367	19.150447308595936
11	27.25108517117415	24.7070136910468	28.52357271911136	19.51832841866769
12	21.428840708535603	30.147161613658714	28.76629622213799	19.6577014556677
13	24.620112562809993	25.25949818452398	29.700199186262992	20.42019006640303
14	25.56650086545298	25.770396445275406	27.99401412590961	20.669088563362003
15	24.181538541222597	27.766887897182286	27.187934868148762	20.863638693446358
16	24.734941018674686	26.317844490422765	27.725572013274963	21.221642477627583
17	24.34819841734447	26.36747196701962	28.013127766385427	21.27120184925048
18	24.06348209530486	26.35470630102426	28.185657009693994	21.39615459397688
19	23.668886984664287	26.756013206268186	28.48701233161539	21.08808747745214
20	23.24619473308857	26.845981304488966	28.577855556080685	21.32996840634178
21	23.286493068008003	26.970773796931425	28.41343604904636	21.32929708601421
22	22.95346836539715	27.178941663246984	28.66970688326715	21.197883088088716
23	22.931985688885245	27.293239300747146	28.49124092285776	21.28353408750985
24	22.739439516637194	27.404629294239896	28.419101486056874	21.43682970306604
25	22.776699559696915	27.584169426947923	28.25728456540239	21.381846447952775
26	22.75327594261706	27.52307540055254	28.249288564880658	21.474360091949745
27	22.750961336800223	27.5424736768828	28.151242123479697	21.55532286283728
28	22.706178568940278	27.59824590843727	28.10802591603591	21.587549606586542
29	22.816431263497925	27.554649578775454	27.96356655631352	21.6653526014131
30	22.794233117811423	27.527537672060326	27.95361626482776	21.724612945300485
31	22.89121953599173	27.504897878438157	27.83712661432986	21.766755971240254
32	22.9251697920044	27.55176812808523	27.765194487178885	21.757867592731483
33	22.886632534351946	27.490709962332637	27.737591552305997	21.885065951009416
34	22.89465328012669	27.533385613409127	27.72113926773063	21.85082183873355
35	22.997215397412724	27.48497891139941	27.634542285447427	21.88326340574044
36	22.953650221218563	27.458219070493694	27.681521984986652	21.90660872330109
37	22.91607283362812	27.428680830399	27.67347081632201	21.981775519650867
38	22.875512093976997	27.478095080952297	27.774134536646045	21.872258288424657
39	22.867591215072828	27.38116181763564	27.714966223097964	22.03628074419357
40	22.8792245516949	27.429081854148357	27.72756142627099	21.964132167885754
41	22.908926188047214	27.419189149565327	27.721200980703	21.950683681684463
42	22.790843359590603	27.399962780977464	27.73642084252581	22.072773016906122
43	22.871004225819245	27.479574826536663	27.61420867241073	22.035212275233366
44	22.917392723109767	27.35771800865463	27.692833907314867	22.032055360920737
45	22.903275926342104	27.39179232480482	27.597459259638978	22.107472489214096
46	22.773562930913997	27.49653949791837	27.557156942298583	22.172740628869047
47	22.837145657982674	27.410977770281498	27.602303120073962	22.149573451661873
48	22.774639798332096	27.314117926504366	27.53873636628006	22.37250590888348
49	22.835358856103767	27.40785287400631	27.53990601100934	22.216882258880577
50	22.74636397588213	27.420657530422037	27.568799392578725	22.264179101117104
51	22.801027154930978	27.37033983847445	27.530541690523602	22.29809131607097
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.4140544198276
2	39.363973845276625
3	41.3702154301741
4	42.4776252597453
5	46.89851077464332
6	40.20915461396168
7	44.809130170342726
8	42.06512701273218
9	41.93773928556857
10	41.94011930870879
11	46.76941358984183
12	41.0865421642033
13	45.040302629213016
14	46.23558942881498
15	45.045177234668955
16	45.956583496302265
17	45.61940026659495
18	45.45963668928174
19	44.756974462116425
20	44.57616313943035
21	44.615790154022214
22	44.151351453485866
23	44.21551977639509
24	44.17626921970324
25	44.15854600764969
26	44.2276360345668
27	44.3062841996375
28	44.29372817552682
29	44.48178386491103
30	44.51884606311191
31	44.65797550723198
32	44.68303738473588
33	44.77169848536136
34	44.74547511886024
35	44.88047880315316
36	44.86025894451966
37	44.89784835327899
38	44.74777038240166
39	44.9038719592664
40	44.843356719580655
41	44.85960986973168
42	44.86361637649673
43	44.90621650105261
44	44.9494480840305
45	45.0107484155562
46	44.94630355978304
47	44.98671910964455
48	45.14714570721557
49	45.05224111498435
50	45.010543076999234
51	45.09911847100195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22327.0
1	13750.0
2	5173.0
3	6618.0
4	8063.0
5	27208.5
6	46354.0
7	59079.5
8	71805.0
9	52996.0
10	34187.0
11	33035.0
12	31883.0
13	39111.0
14	46339.0
15	61734.0
16	77129.0
17	104015.5
18	130902.0
19	166254.0
20	201606.0
21	246452.5
22	291299.0
23	353143.5
24	414988.0
25	490248.5
26	659352.0
27	753195.0
28	840103.0
29	927011.0
30	1016085.5
31	1105160.0
32	1206368.5
33	1307577.0
34	1401300.0
35	1495023.0
36	1619476.0
37	1743929.0
38	1836095.5
39	1928262.0
40	2016596.5
41	2104931.0
42	2150429.0
43	2195927.0
44	2214976.5
45	2234026.0
46	2214930.5
47	2195835.0
48	2141085.5
49	2086336.0
50	1990125.0
51	1893914.0
52	1757556.5
53	1621199.0
54	1487437.0
55	1353675.0
56	1227095.5
57	1100516.0
58	974478.5
59	848441.0
60	753685.0
61	658929.0
62	572331.0
63	485733.0
64	422393.0
65	359053.0
66	303568.5
67	248084.0
68	234145.0
69	220206.0
70	198138.5
71	176071.0
72	151377.5
73	126684.0
74	107779.0
75	75679.0
76	62484.0
77	58919.0
78	55354.0
79	55356.0
80	55358.0
81	48697.0
82	42036.0
83	39046.0
84	36056.0
85	32460.0
86	28864.0
87	33713.5
88	38563.0
89	26266.0
90	13969.0
91	9313.0
92	4657.0
93	3573.0
94	2489.0
95	1916.0
96	1343.0
97	1003.0
98	663.0
99	403.0
100	143.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1728110448624315E-4
2	0.0034803960196187833
3	0.0
4	0.00585137618598932
5	0.013794793695246763
6	0.007544027802088073
7	0.025773315150747107
8	0.006605778966198128
9	0.012593454814157948
10	0.007097091701208065
11	0.025925463610621154
12	0.02852466646680276
13	0.012013388810888148
14	0.014381199217677977
15	0.001575370511612509
16	0.04115298863634851
17	0.002060343727461028
18	4.8180345626780966E-4
19	0.00602888272250904
20	0.0010999065745061181
21	0.0011030763340868274
22	0.01650176837717248
23	0.0010999065745061181
24	0.020948941068907594
25	0.003892464765110989
26	0.00423479879982759
27	0.007854664240997582
28	0.001293261908929384
29	0.059436161897879605
30	0.006580420889552453
31	9.224000379863988E-4
32	0.005990845607540528
33	0.009674106240324705
34	0.01650176837717248
35	0.013677512590760518
36	0.011217779156130122
37	0.016409845349331913
38	0.027757584648271112
39	0.020948941068907594
40	0.022020319807187327
41	0.02634070211569407
42	0.01624184809155432
43	0.022286579611966905
44	0.01590268381641843
45	0.013994488548831445
46	0.02966261015627739
47	0.02083799948358277
48	0.04787604870703288
49	0.012723414956967025
50	0.04491866301823112
51	0.0963480122152391
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.1548134E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.7048561999057
#Duplication Level	Relative count
1	100.0
2	10.798959258789186
3	2.968650844015738
4	1.3973854550069806
5	0.8801878410965859
6	0.5850996319329864
7	0.4543723822820155
8	0.3769513897702754
9	0.32364513263104455
10++	9.134407919786774
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	8535325	4.517308	44.980816	4
TTTTT	12483040	4.266809	5.6151924	18
AAAAA	7289625	3.4994378	3.7042077	44
GTTTG	6275900	3.321511	44.453995	7
TGTTT	7614975	3.2388418	36.23447	6
GTGTT	5987930	3.169103	44.363495	5
TTTGA	6727750	3.0626202	38.39773	8
CTTTG	5084185	2.934528	48.59038	1
TTGTG	5523590	2.923352	44.43561	3
GGGGG	2621625	2.6732862	5.709844	13
TTTGT	5831065	2.4800997	35.963444	2
TTGAG	4161115	2.3570642	27.958176	9
GAGGG	2659780	2.3328362	12.28371	11
TGAGG	2980220	2.1006243	16.267765	10
AGGGG	1940440	1.7019184	6.7811275	12
ACTTT	3401845	1.6888654	17.55497	3
AACTT	3042320	1.6165439	19.299944	2
TGAGC	1945715	1.4956727	6.339103	10
TGAGA	2453710	1.4876027	5.3385167	10
TGAGT	2355920	1.334511	8.0196295	10
GAGTG	1861795	1.3122964	6.2485805	11
TAACT	2395895	1.2730643	19.164312	1
TTGAT	2648700	1.2057467	8.603378	9
TTGAC	1732400	1.0702053	7.9317846	9
GTAAC	1124160	0.74327433	5.8721304	1
>>END_MODULE