FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_7_ACTTGA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_7_ACTTGA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 31548134
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35120 0.11132195647450972 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11545230 4.0949516 5.273705 18
TGTGT 6884105 3.8547661 51.68089 4
AAAAA 7235455 3.3046505 3.4307594 44
TGTTT 6200060 2.7630851 41.081474 6
GTTTG 4849575 2.7155275 50.83543 7
CTTTG 4477120 2.6451914 53.70006 1
GTGTT 4548770 2.5470915 50.843197 5
TTTGA 5363010 2.5140254 42.81578 8
TTGTG 4163185 2.3311825 50.88441 3
TTTGT 5175940 2.3066814 40.798355 2
GAGGG 2324885 2.161818 13.765897 11
GGGGG 1920225 2.1328447 5.488521 13
GAGAG 2662265 2.0724277 5.036227 11
TTGAG 3367450 1.9834186 31.369003 9
TGAGG 2615525 1.9356388 18.572397 10
AGGGG 1703465 1.5839844 7.4644938 12
TGAGA 2349885 1.4558699 5.815376 10
TGAGC 1855510 1.4488937 6.871154 10
GAGTG 1731195 1.2811838 6.9969516 11
TGAGT 2163825 1.274487 8.929217 10
TTGAT 2364230 1.1082833 9.590013 9
TTGAC 1556655 0.96741635 8.717339 9
Produced by FastQC (version 0.10.1)