##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_7_ACTTGA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	31548134	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.492077566299166	34.0	31.0	34.0	31.0	34.0
2	32.78823571625504	34.0	31.0	34.0	31.0	34.0
3	32.905006647936766	34.0	31.0	34.0	31.0	34.0
4	36.38447053635565	37.0	37.0	37.0	35.0	37.0
5	36.29658815954059	37.0	37.0	37.0	35.0	37.0
6	36.263679430295305	37.0	37.0	37.0	35.0	37.0
7	36.27310784847053	37.0	37.0	37.0	35.0	37.0
8	36.266303547461796	37.0	37.0	37.0	35.0	37.0
9	38.0259127845723	39.0	38.0	39.0	35.0	39.0
10	38.02604071606898	39.0	38.0	39.0	35.0	39.0
11	38.01203358017942	39.0	38.0	39.0	35.0	39.0
12	37.99112647993697	39.0	38.0	39.0	35.0	39.0
13	37.976802589972515	39.0	38.0	39.0	35.0	39.0
14	39.45754278208657	41.0	39.0	41.0	37.0	41.0
15	39.47978336848703	41.0	39.0	41.0	37.0	41.0
16	39.456969911437554	41.0	39.0	41.0	37.0	41.0
17	39.44120153033457	41.0	39.0	41.0	37.0	41.0
18	39.40400430656216	41.0	39.0	41.0	36.0	41.0
19	39.41765953574306	41.0	39.0	41.0	37.0	41.0
20	39.38650438089302	41.0	39.0	41.0	36.0	41.0
21	39.361173912853296	41.0	39.0	41.0	36.0	41.0
22	39.33590474796386	41.0	39.0	41.0	36.0	41.0
23	39.303255526935445	40.0	39.0	41.0	36.0	41.0
24	39.255468136403884	41.0	39.0	41.0	36.0	41.0
25	39.20792954030181	40.0	39.0	41.0	36.0	41.0
26	39.1160212201457	40.0	39.0	41.0	36.0	41.0
27	39.063308815665614	40.0	39.0	41.0	36.0	41.0
28	38.99083083646088	40.0	39.0	41.0	35.0	41.0
29	38.93622456402651	40.0	39.0	41.0	35.0	41.0
30	38.87155386115705	40.0	38.0	41.0	35.0	41.0
31	38.8003249257151	40.0	38.0	41.0	35.0	41.0
32	38.70674110234222	40.0	38.0	41.0	35.0	41.0
33	38.62988261048974	40.0	38.0	41.0	35.0	41.0
34	38.77090876436622	40.0	38.0	41.0	35.0	41.0
35	38.79224872697701	40.0	38.0	41.0	35.0	41.0
36	38.78851636676832	40.0	38.0	41.0	35.0	41.0
37	38.74298140739481	40.0	38.0	41.0	35.0	41.0
38	38.697255216425795	40.0	38.0	41.0	35.0	41.0
39	38.45572204048582	40.0	38.0	41.0	34.0	41.0
40	38.39329403761249	40.0	38.0	41.0	34.0	41.0
41	38.442958211094194	40.0	38.0	41.0	34.0	41.0
42	38.19776063459094	40.0	38.0	41.0	34.0	41.0
43	38.214303197773916	40.0	38.0	41.0	34.0	41.0
44	38.18589901386878	40.0	38.0	41.0	34.0	41.0
45	38.199972207547994	40.0	38.0	41.0	34.0	41.0
46	38.15756922422099	40.0	38.0	41.0	34.0	41.0
47	38.08516367402269	40.0	38.0	41.0	33.0	41.0
48	38.03172983226203	40.0	38.0	41.0	33.0	41.0
49	37.89225720925364	40.0	37.0	41.0	33.0	41.0
50	37.70993114838424	40.0	37.0	41.0	33.0	41.0
51	37.60790755484936	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	4.0
11	17.0
12	40.0
13	89.0
14	192.0
15	335.0
16	544.0
17	992.0
18	1699.0
19	3252.0
20	6053.0
21	10620.0
22	17809.0
23	27923.0
24	41735.0
25	59103.0
26	81331.0
27	109626.0
28	143170.0
29	187535.0
30	242708.0
31	312385.0
32	404053.0
33	536508.0
34	765825.0
35	1153679.0
36	1750276.0
37	2907548.0
38	6390051.0
39	1.6384624E7
40	8403.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.394044858184795	27.41895373770804	28.301566186849463	25.885435217257708
2	20.22734847011871	27.014532777120827	32.915252610503046	19.842866142257414
3	19.362057706572667	25.87084810022253	34.36006061028726	20.407033582917535
4	20.512706447550062	25.665920000121755	33.743622615200685	20.077750937127497
5	27.171328104540194	25.588347000174398	27.946280436110737	19.29404445917467
6	21.726267550404092	25.25795028003875	32.897720670262146	20.118061499295013
7	27.047732838969175	25.893575829239218	27.381140830706503	19.677550501085104
8	21.687149547418556	25.86404634898533	32.37503365492235	20.073770448673763
9	20.94382190718475	26.229633739986014	32.73243989644522	20.094104456384013
10	21.39672349559565	25.915535923614375	32.5838700951378	20.103870485652177
11	26.935510670773745	25.76488992978158	27.137107380106855	20.162492019337815
12	21.35540001685678	31.569338825850647	26.775048780224175	20.300212377068398
13	24.51078959975379	26.38641880169108	28.04054609854462	21.06224550001051
14	23.619086314265054	26.71811587969038	28.436293569692584	21.226504236351982
15	23.902421613905915	26.691860761083362	28.025952343171866	21.379765281838857
16	23.72902308580279	26.681204029373024	28.022671642005832	21.567101242818353
17	23.63346310915737	26.817616858080857	27.917533146571543	21.63138688619023
18	23.323394339905587	26.86004124935325	28.193321499774427	21.623242910966738
19	22.930883962899358	27.222675039988104	28.536340691338513	21.31010030577403
20	22.572634565328016	27.27516308888507	28.68178510969936	21.47041723608756
21	22.751542135582408	27.290542762370666	28.375484901896257	21.582430200150664
22	22.556623275693223	27.392975928544445	28.50646883380949	21.543931961952843
23	22.732552105934378	27.41353577362135	28.287194418535183	21.566717701909088
24	22.46500537876503	27.517158384074314	28.29955965065953	21.718276586501123
25	22.56584494030614	27.63797377049305	28.15512004608577	21.641061243115047
26	22.583281787759617	27.52838250274961	28.090200200113262	21.798135509377513
27	22.62503959600018	27.565292231240196	27.96349176667883	21.84617640608079
28	22.571376170774474	27.66567746922845	27.99786193376762	21.765084426229457
29	22.708095819202935	27.586848827741665	27.881791889805935	21.82326346324946
30	22.62311615641039	27.526632795461058	27.946527677358034	21.90372337077052
31	22.759189191699296	27.486104323861042	27.78703506386101	21.96767142057865
32	22.777552463455493	27.587846644781628	27.741019526562656	21.893581365200223
33	22.727930885919115	27.49829213018152	27.69949301497727	22.074283968922096
34	22.679491484774612	27.62099628324056	27.732669603498532	21.966842628486287
35	22.809715594096296	27.52889123988939	27.634305887212797	22.02708727880152
36	22.819298101246805	27.47988190911734	27.67400673048041	22.026813259155446
37	22.822137247664315	27.42048189734977	27.695779812384338	22.061601042601584
38	22.79039619201048	27.460976431268865	27.74430672056213	22.004320656158527
39	22.810680767085916	27.557514374453074	27.573376014797095	22.05842884366391
40	22.80477507798084	27.50604837674393	27.690186050306494	21.998990494968737
41	22.788422488597806	27.381940009324374	27.763898150074702	22.06573935200312
42	22.786489436967948	27.512517528978286	27.581267259545527	22.119725774508236
43	22.74851319465845	27.58278363382193	27.594007856385232	22.07469531513439
44	22.834158000006337	27.399019275328783	27.62543227642869	22.141390448236187
45	22.85889555306187	27.318376421248875	27.576711827076682	22.24601619861257
46	22.697418490741796	27.479831295251884	27.626775009894406	22.195975204111914
47	22.735712373892436	27.529188158209877	27.591974887337003	22.143124580560684
48	22.68468187805228	27.354732459256848	27.592999957903942	22.36758570478693
49	22.856001561298513	27.413467924010256	27.52615934164528	22.20437117304596
50	22.802616831542284	27.636240902451803	27.333203520244425	22.227938745761488
51	22.73056588694215	27.366586000477582	27.545601681354054	22.357246431226212
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.2794800754425
2	40.07021461237613
3	39.7690912894902
4	40.590457384677556
5	46.465372563714865
6	41.844329049699105
7	46.72528334005428
8	41.76091999609232
9	41.03792636356876
10	41.50059398124783
11	47.098002690111564
12	41.65561239392518
13	45.5730350997643
14	44.84559055061704
15	45.28218689574477
16	45.29612432862115
17	45.2648499953476
18	44.94663725087232
19	44.24098426867339
20	44.04305180141558
21	44.33397233573307
22	44.10055523764606
23	44.299269807843466
24	44.183281965266154
25	44.20690618342118
26	44.38141729713713
27	44.47121600208098
28	44.33646059700393
29	44.5313592824524
30	44.52683952718091
31	44.726860612277946
32	44.67113382865572
33	44.80221485484121
34	44.6463341132609
35	44.83680287289782
36	44.846111360402254
37	44.883738290265896
38	44.79471684816901
39	44.86910961074983
40	44.80376557294958
41	44.85416184060093
42	44.90621521147619
43	44.82320850979284
44	44.97554844824253
45	45.10491175167444
46	44.893393694853714
47	44.87883695445312
48	45.052267582839214
49	45.06037273434447
50	45.03055557730377
51	45.08781231816836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36241.0
1	21595.5
2	6950.0
3	8459.0
4	9968.0
5	18568.5
6	27169.0
7	46384.0
8	65599.0
9	49025.5
10	32452.0
11	32451.5
12	32451.0
13	40471.5
14	48492.0
15	65873.5
16	83255.0
17	112701.5
18	142148.0
19	179794.0
20	217440.0
21	264432.0
22	311424.0
23	372790.5
24	434157.0
25	512797.5
26	684858.0
27	778278.0
28	858719.0
29	939160.0
30	1021303.0
31	1103446.0
32	1201267.5
33	1299089.0
34	1390503.5
35	1481918.0
36	1601672.0
37	1721426.0
38	1810339.0
39	1899252.0
40	1985996.0
41	2072740.0
42	2117788.5
43	2162837.0
44	2184513.5
45	2206190.0
46	2185452.0
47	2164714.0
48	2121798.5
49	2078883.0
50	1990783.5
51	1902684.0
52	1768087.5
53	1633491.0
54	1501901.5
55	1370312.0
56	1241415.5
57	1112519.0
58	986629.0
59	860739.0
60	764193.5
61	667648.0
62	579169.5
63	490691.0
64	427507.0
65	364323.0
66	307783.5
67	251244.0
68	238813.5
69	226383.0
70	203068.5
71	179754.0
72	154561.0
73	129368.0
74	109659.0
75	75210.0
76	60470.0
77	57974.0
78	55478.0
79	55020.0
80	54562.0
81	48173.5
82	41785.0
83	37240.0
84	32695.0
85	30239.5
86	27784.0
87	32392.5
88	37001.0
89	23343.0
90	9685.0
91	5658.0
92	1631.0
93	1144.5
94	658.0
95	384.5
96	111.0
97	79.0
98	47.0
99	25.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031516919510992315
2	0.0
3	0.0073982188613754465
4	0.030860779277785494
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	3.169759580709274E-6
13	0.0038892950055302794
14	0.0
15	0.0
16	0.0
17	0.01673316082656426
18	9.509278742127822E-6
19	0.0
20	0.0
21	0.0
22	6.656495119489476E-5
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0043964565384437635
28	0.0
29	3.4867355387802015E-5
30	0.0
31	3.4867355387802015E-5
32	0.0065930999278752895
33	1.96525094003975E-4
34	0.0024090172813390483
35	0.0010650392191183162
36	0.0028718021801226023
37	4.0889898591149636E-4
38	4.691244179449726E-4
39	0.001030171863730514
40	0.0
41	0.0022124921873350736
42	0.0011854900831852686
43	9.255697975671081E-4
44	1.0777182574411533E-4
45	0.0
46	0.0
47	2.0920413232681213E-4
48	0.003924162360918081
49	1.109415853248246E-4
50	6.688192715296569E-4
51	0.08518094921240033
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.1548134E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.898403979056614
#Duplication Level	Relative count
1	100.0
2	11.318423882431256
3	3.085155409903221
4	1.4881714373495827
5	0.935147729018383
6	0.6445542526499053
7	0.47941522863433866
8	0.3725229146397665
9	0.32963797429463876
10++	9.361718469967741
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35120	0.11132195647450972	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11545230	4.0949516	5.273705	18
TGTGT	6884105	3.8547661	51.68089	4
AAAAA	7235455	3.3046505	3.4307594	44
TGTTT	6200060	2.7630851	41.081474	6
GTTTG	4849575	2.7155275	50.83543	7
CTTTG	4477120	2.6451914	53.70006	1
GTGTT	4548770	2.5470915	50.843197	5
TTTGA	5363010	2.5140254	42.81578	8
TTGTG	4163185	2.3311825	50.88441	3
TTTGT	5175940	2.3066814	40.798355	2
GAGGG	2324885	2.161818	13.765897	11
GGGGG	1920225	2.1328447	5.488521	13
GAGAG	2662265	2.0724277	5.036227	11
TTGAG	3367450	1.9834186	31.369003	9
TGAGG	2615525	1.9356388	18.572397	10
AGGGG	1703465	1.5839844	7.4644938	12
TGAGA	2349885	1.4558699	5.815376	10
TGAGC	1855510	1.4488937	6.871154	10
GAGTG	1731195	1.2811838	6.9969516	11
TGAGT	2163825	1.274487	8.929217	10
TTGAT	2364230	1.1082833	9.590013	9
TTGAC	1556655	0.96741635	8.717339	9
>>END_MODULE