FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_3_GTCCGC_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_3_GTCCGC_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 35762372
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11094720 3.6056707 4.424518 17
TGTGT 6544215 3.3639414 24.889542 4
AAAAA 8233090 3.0598154 3.279675 11
TGTTT 6069300 2.48067 19.88005 6
GTTTG 4432355 2.2783766 24.130781 7
CTTTG 4046430 2.1799624 25.45986 1
GTGTT 4113215 2.1143277 24.07753 5
TTTGA 5033725 2.1133544 19.943483 8
GAGGG 2420580 2.0215476 7.275525 11
TTGTG 3915235 2.0125592 24.12011 3
TTTGT 4827285 1.9730281 19.551239 2
TGAGG 2747755 1.8246602 9.180112 10
TTGAG 3252370 1.7172886 14.922994 9
ACTTT 3084595 1.3572724 6.728626 3
AACTT 2798765 1.2649922 7.085304 2
TAACT 2079790 0.94002825 6.8596077 1
Produced by FastQC (version 0.10.1)