##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_3_GTCCGC_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	35762372	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.176882842111254	33.0	31.0	34.0	31.0	34.0
2	32.339843061864016	34.0	31.0	34.0	31.0	34.0
3	32.423991646862795	34.0	31.0	34.0	31.0	34.0
4	35.908546222828846	37.0	37.0	37.0	35.0	37.0
5	35.924401966402	37.0	37.0	37.0	35.0	37.0
6	35.9346415556552	37.0	37.0	37.0	35.0	37.0
7	35.951826070149934	37.0	37.0	37.0	35.0	37.0
8	35.946389769671875	37.0	37.0	37.0	35.0	37.0
9	37.67254504259393	39.0	38.0	39.0	35.0	39.0
10	37.65655678543918	39.0	38.0	39.0	35.0	39.0
11	37.64968707892194	39.0	38.0	39.0	35.0	39.0
12	37.592698353453734	39.0	38.0	39.0	35.0	39.0
13	37.56463651236557	39.0	38.0	39.0	35.0	39.0
14	39.052750415995895	41.0	39.0	41.0	36.0	41.0
15	39.01520880661943	41.0	39.0	41.0	36.0	41.0
16	38.97842427789745	41.0	39.0	41.0	36.0	41.0
17	38.96243800606962	41.0	39.0	41.0	36.0	41.0
18	38.9336752606902	41.0	39.0	41.0	36.0	41.0
19	38.9362874196376	41.0	39.0	41.0	36.0	41.0
20	38.9107821203806	41.0	39.0	41.0	36.0	41.0
21	38.86150437672311	41.0	39.0	41.0	35.0	41.0
22	38.82032746597457	40.0	39.0	41.0	35.0	41.0
23	38.79050114461088	40.0	39.0	41.0	35.0	41.0
24	38.749538761019544	40.0	39.0	41.0	35.0	41.0
25	38.67781166752586	40.0	39.0	41.0	35.0	41.0
26	38.605399328657505	40.0	39.0	41.0	34.0	41.0
27	38.57223578458387	40.0	39.0	41.0	34.0	41.0
28	38.529922651663036	40.0	38.0	41.0	34.0	41.0
29	38.445554142773304	40.0	38.0	41.0	34.0	41.0
30	38.40281111666754	40.0	38.0	41.0	34.0	41.0
31	38.34124151496439	40.0	38.0	41.0	34.0	41.0
32	38.29002698702424	40.0	38.0	41.0	34.0	41.0
33	38.21899758215143	40.0	38.0	41.0	34.0	41.0
34	38.16242938248056	40.0	38.0	41.0	33.0	41.0
35	38.10352694166931	40.0	38.0	41.0	33.0	41.0
36	38.049791523895564	40.0	38.0	41.0	33.0	41.0
37	37.979516711028005	40.0	38.0	41.0	33.0	41.0
38	37.921761118082436	40.0	38.0	41.0	33.0	41.0
39	37.83070915989577	40.0	38.0	41.0	33.0	41.0
40	37.752069297864246	40.0	38.0	41.0	33.0	41.0
41	37.93774948708659	40.0	38.0	41.0	33.0	41.0
42	37.98081310154707	40.0	38.0	41.0	33.0	41.0
43	37.96293436576299	40.0	38.0	41.0	33.0	41.0
44	37.926807259876384	40.0	38.0	41.0	33.0	41.0
45	37.863557875859016	40.0	38.0	41.0	33.0	41.0
46	37.819403645820806	40.0	38.0	41.0	33.0	41.0
47	37.7654278636775	40.0	38.0	41.0	33.0	41.0
48	37.70300977239429	40.0	37.0	41.0	33.0	41.0
49	37.63457105138328	40.0	37.0	41.0	32.0	41.0
50	37.55445967062811	40.0	37.0	41.0	32.0	41.0
51	36.058397999998434	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	14.0
6	338.0
7	2146.0
8	6699.0
9	11097.0
10	13957.0
11	18573.0
12	27139.0
13	37944.0
14	46806.0
15	53080.0
16	56955.0
17	59176.0
18	60825.0
19	63367.0
20	67077.0
21	72615.0
22	79438.0
23	88986.0
24	100964.0
25	116051.0
26	134235.0
27	155968.0
28	186443.0
29	223393.0
30	275572.0
31	345752.0
32	448050.0
33	598909.0
34	848037.0
35	1248588.0
36	1889755.0
37	3124816.0
38	7094904.0
39	1.8201841E7
40	2862.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.18557056354765	28.078831949340692	28.822516236557476	22.91308125055418
2	22.261377739422013	28.832576244434527	29.964180074763913	18.941865941379547
3	22.10285995570987	27.5317000785071	30.08458443416449	20.280855531618542
4	21.891847980801014	26.44777632799798	30.596836372930593	21.063539318270415
5	25.080103895952803	26.682430715149952	28.28341794819476	19.954047440702478
6	22.04958863869123	26.359025609930605	30.903025622458678	20.688360128919488
7	24.214223100367832	26.879187798972172	28.684493232604407	20.222095868055597
8	22.004633723666007	26.791192921679635	30.584391072161083	20.619782282493272
9	22.09251869830427	26.72377832767508	30.600544977782185	20.58315799623847
10	22.07319580078473	27.576391868070367	29.89577186113463	20.454640470010276
11	24.359283065060072	26.738888041253002	28.305650135715748	20.596178757971177
12	21.61187686316505	29.30964223846616	28.37623988521593	20.702241013152857
13	23.17950420276993	27.02051417075888	28.840284954694713	20.959696671776484
14	23.2982361682647	27.25889204607664	28.341289164934548	21.10158262072412
15	22.88953778161179	27.8465847961965	28.011537458641243	21.25233996355047
16	23.029449350173575	27.38016478110134	28.184418589180922	21.405967279544168
17	22.943649283740182	27.37900402901391	28.24745221159543	21.429894475650478
18	22.77095116847177	27.349737776067467	28.310059492454908	21.56925156300586
19	22.62036785309187	27.558970917824016	28.414943585132253	21.405717643951856
20	22.442377439220316	27.558236340924086	28.44049426666429	21.55889195319131
21	22.490023827830704	27.58655893693531	28.33136704492424	21.592050190309745
22	22.395167630271192	27.679367340700413	28.385691836167933	21.539773192860462
23	22.4131017511941	27.72048138921097	28.289750477587972	21.576666382006955
24	22.33759755297771	27.737442715038874	28.233096403859363	21.691863328124057
25	22.38158767011867	27.856197952892416	28.13397827068169	21.628236106307227
26	22.3930432683639	27.81324676707601	28.104299968993995	21.6894099955661
27	22.386969623532885	27.781357871811146	28.045878100277694	21.785794404378276
28	22.364326941333356	27.821999899893434	28.03399932162422	21.779673837148987
29	22.39486787606199	27.83461192681671	27.97667631501401	21.793843882107293
30	22.405678600290678	27.78432591845305	27.959480020365806	21.850515460890467
31	22.482901079532542	27.788586410293615	27.87812265699623	21.850389853177614
32	22.501194623923887	27.81104628921621	27.839197691467565	21.84856139539233
33	22.477661864181282	27.75818561609042	27.806819642038917	21.95733287768938
34	22.487173013898946	27.791130613046576	27.799048065480548	21.922648307573926
35	22.513132696862787	27.796417686501467	27.758699732618386	21.931749884017364
36	22.51084753448956	27.764486711861135	27.75936623037144	21.96529952327786
37	22.526256687978126	27.753016940216398	27.746218178066545	21.974508193738938
38	22.51831028335676	27.780509535690474	27.79420375419616	21.9069764267566
39	22.492407974068147	27.71943822378267	27.764813760467117	22.02334004168206
40	22.523406101599804	27.72691092878793	27.766047306155578	21.98363566345669
41	22.514964817662282	27.735303516275895	27.76442552763018	21.985306138431643
42	22.46127302588764	27.71586628953165	27.760252498265913	22.062608186314797
43	22.5235096610352	27.759800528325844	27.679558768209272	22.037131042429685
44	22.549065588557596	27.69957746914919	27.720527401328294	22.03082954096492
45	22.55234633596483	27.708033872555237	27.651114068205214	22.08850572327472
46	22.48399015663607	27.772701858733644	27.632929089409398	22.110378895220883
47	22.495667154209706	27.74361545719522	27.662082330111165	22.09863505848391
48	22.488628674104575	27.669037373774565	27.632520554104484	22.209813398016383
49	22.523931274094735	27.732353981837594	27.616474408445345	22.127240335622325
50	22.45274257925305	27.72882671613253	27.678245019628978	22.140185684985443
51	22.494585022053162	27.696434542176927	27.64724029391828	22.161740141851627
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.09865181410183
2	41.20324368080156
3	42.38371548732841
4	42.955387299071425
5	45.034151336655285
6	42.73794876761072
7	44.43631896842343
8	42.62441600615928
9	42.675676694542744
10	42.52783627079501
11	44.95546182303124
12	42.31411787631791
13	44.13920087454641
14	44.39981878898882
15	44.141877745162255
16	44.43541662971774
17	44.37354375939066
18	44.340202731477625
19	44.026085497043724
20	44.00126939241162
21	44.082074018140446
22	43.934940823131654
23	43.98976813320105
24	44.02946088110176
25	44.0098237764259
26	44.08245326392999
27	44.17276402791116
28	44.144000778482344
29	44.18871175816928
30	44.25619406118114
31	44.333290932710156
32	44.34975601931622
33	44.43499474187067
34	44.40982132147287
35	44.444882580880154
36	44.47614705776742
37	44.50076488171706
38	44.42528671011337
39	44.51574801575021
40	44.50704176505649
41	44.50027095609392
42	44.52388121220244
43	44.560640703464884
44	44.57989512952252
45	44.640852059239556
46	44.59436905185695
47	44.59430221269361
48	44.69844207212096
49	44.65117160971706
50	44.59292826423849
51	44.65632516390479
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16966.0
1	11067.5
2	5169.0
3	7153.0
4	9137.0
5	19251.5
6	29366.0
7	36563.5
8	43761.0
9	36013.5
10	28266.0
11	31261.0
12	34256.0
13	44821.0
14	55386.0
15	74451.5
16	93517.0
17	127318.0
18	161119.0
19	205097.0
20	249075.0
21	303553.5
22	358032.0
23	428098.0
24	498164.0
25	587385.0
26	784915.5
27	893225.0
28	988361.0
29	1083497.0
30	1178780.0
31	1274063.0
32	1384969.0
33	1495875.0
34	1605292.0
35	1714709.0
36	1856060.5
37	1997412.0
38	2101609.0
39	2205806.0
40	2306712.5
41	2407619.0
42	2451723.0
43	2495827.0
44	2517349.5
45	2538872.0
46	2515070.5
47	2491269.0
48	2428731.5
49	2366194.0
50	2259567.5
51	2152941.0
52	2004351.5
53	1855762.0
54	1703654.5
55	1551547.0
56	1402809.5
57	1254072.0
58	1108302.0
59	962532.0
60	846325.5
61	730119.0
62	628725.5
63	527332.0
64	452847.5
65	378363.0
66	316863.5
67	255364.0
68	233479.0
69	211594.0
70	187461.0
71	163328.0
72	140493.5
73	117659.0
74	99756.0
75	69656.0
76	57459.0
77	53731.0
78	50003.0
79	49149.5
80	48296.0
81	42281.5
82	36267.0
83	32728.0
84	29189.0
85	26344.5
86	23500.0
87	28250.0
88	33000.0
89	22475.5
90	11951.0
91	7769.0
92	3587.0
93	2685.0
94	1783.0
95	1402.5
96	1022.0
97	773.5
98	525.0
99	330.5
100	136.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.312846698199997E-5
2	0.0035512185824810505
3	0.0
4	0.006017497944487574
5	0.014148949627837883
6	0.007519076195505153
7	0.02587356342023398
8	0.006607503551498206
9	0.012817941718183571
10	0.007390449380706627
11	0.02610844716899651
12	0.029005346737067665
13	0.012323008104719676
14	0.01443696184358241
15	0.0016693523572765252
16	0.041456981656585866
17	0.002147508560114525
18	5.312846698199996E-4
19	0.0059224259509408385
20	0.001065365574744315
21	0.001143660157665157
22	0.016783003096103356
23	0.0010457919290141045
24	0.021164703504566194
25	0.0038560082088514715
26	0.004350941822315366
27	0.008148229093976205
28	0.0013477853202802097
29	0.0602197192065448
30	0.006803240008800311
31	8.919989982767362E-4
32	0.006051052765739365
33	0.009666584755619677
34	0.016556508052653778
35	0.013603683782496307
36	0.011229680178932203
37	0.01639432641660346
38	0.02812453267920819
39	0.02075645317933609
40	0.022126608380450826
41	0.026136409520039663
42	0.016134276551902096
43	0.022151774496389668
44	0.015918966448869778
45	0.014109802336377463
46	0.03012384077879398
47	0.02119546209071367
48	0.04809524379423155
49	0.012750832075679992
50	0.04541085809408839
51	0.09680006684120393
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.5762372E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.58104043025816
#Duplication Level	Relative count
1	100.0
2	11.610198603035663
3	3.375361009682057
4	1.6259748851766562
5	0.9535744729576712
6	0.6660390081298744
7	0.4656547793228922
8	0.37214213921296707
9	0.30407511545948424
10++	7.855061769233705
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11094720	3.6056707	4.424518	17
TGTGT	6544215	3.3639414	24.889542	4
AAAAA	8233090	3.0598154	3.279675	11
TGTTT	6069300	2.48067	19.88005	6
GTTTG	4432355	2.2783766	24.130781	7
CTTTG	4046430	2.1799624	25.45986	1
GTGTT	4113215	2.1143277	24.07753	5
TTTGA	5033725	2.1133544	19.943483	8
GAGGG	2420580	2.0215476	7.275525	11
TTGTG	3915235	2.0125592	24.12011	3
TTTGT	4827285	1.9730281	19.551239	2
TGAGG	2747755	1.8246602	9.180112	10
TTGAG	3252370	1.7172886	14.922994	9
ACTTT	3084595	1.3572724	6.728626	3
AACTT	2798765	1.2649922	7.085304	2
TAACT	2079790	0.94002825	6.8596077	1
>>END_MODULE