FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_3_GTCCGC_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_3_GTCCGC_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 35762372
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 10937470 3.4556062 4.2016783 16
TGTGT 5923390 3.095253 27.167248 4
TGTTT 5546070 2.2534707 21.118847 6
CTTTG 3845190 2.0675402 27.132006 1
GTTTG 3855900 2.014891 26.214273 7
GAGGG 2227230 1.9754335 8.091859 11
TTTGT 4656210 1.8919041 20.841423 2
TTTGA 4493480 1.8737109 21.175371 8
GTGTT 3522020 1.840423 26.216732 5
TTGTG 3412410 1.7831464 26.284231 3
TGAGG 2577435 1.7775664 10.221328 10
TTGAG 2928665 1.5705388 16.114656 9
TGAGT 2245025 1.2039269 5.0376573 10
TTGAT 2344490 0.97761565 5.1743875 9
Produced by FastQC (version 0.10.1)