##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_3_GTCCGC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	35762372	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44033594863338	34.0	31.0	34.0	31.0	34.0
2	32.74699085955484	34.0	31.0	34.0	31.0	34.0
3	32.87336983687771	34.0	31.0	34.0	31.0	34.0
4	36.36315082791488	37.0	37.0	37.0	35.0	37.0
5	36.273947796303894	37.0	37.0	37.0	35.0	37.0
6	36.261749779908335	37.0	37.0	37.0	35.0	37.0
7	36.25796267093246	37.0	37.0	37.0	35.0	37.0
8	36.26128479397284	37.0	37.0	37.0	35.0	37.0
9	38.00418903980977	39.0	38.0	39.0	35.0	39.0
10	37.99807291865316	39.0	38.0	39.0	35.0	39.0
11	37.99206302646815	39.0	38.0	39.0	35.0	39.0
12	37.97606688393041	39.0	38.0	39.0	35.0	39.0
13	37.95537317826681	39.0	38.0	39.0	35.0	39.0
14	39.44351252763659	41.0	39.0	41.0	37.0	41.0
15	39.46743647205504	41.0	39.0	41.0	37.0	41.0
16	39.44779854087978	41.0	39.0	41.0	37.0	41.0
17	39.434002643896214	41.0	39.0	41.0	37.0	41.0
18	39.40530449154771	41.0	39.0	41.0	36.0	41.0
19	39.42515669821901	41.0	39.0	41.0	37.0	41.0
20	39.396929180200914	41.0	39.0	41.0	36.0	41.0
21	39.369750949405706	41.0	39.0	41.0	36.0	41.0
22	39.34644007953388	41.0	39.0	41.0	36.0	41.0
23	39.314014042469	40.0	39.0	41.0	36.0	41.0
24	39.26984588717997	41.0	39.0	41.0	36.0	41.0
25	39.226047701757594	40.0	39.0	41.0	36.0	41.0
26	39.14042614399291	40.0	39.0	41.0	36.0	41.0
27	39.09156928964332	40.0	39.0	41.0	36.0	41.0
28	39.02386382536371	40.0	39.0	41.0	36.0	41.0
29	38.970534197228304	40.0	39.0	41.0	35.0	41.0
30	38.910760058085636	40.0	38.0	41.0	35.0	41.0
31	38.84171947543077	40.0	38.0	41.0	35.0	41.0
32	38.75452126050252	40.0	38.0	41.0	35.0	41.0
33	38.68036440647729	40.0	38.0	41.0	35.0	41.0
34	38.81763399251034	40.0	38.0	41.0	35.0	41.0
35	38.840555179058036	40.0	38.0	41.0	35.0	41.0
36	38.84093915806256	40.0	38.0	41.0	35.0	41.0
37	38.80078471864226	40.0	38.0	41.0	35.0	41.0
38	38.760805659087715	40.0	38.0	41.0	35.0	41.0
39	38.525275784279636	40.0	38.0	41.0	35.0	41.0
40	38.46742643357102	40.0	38.0	41.0	34.0	41.0
41	38.52100831007518	40.0	38.0	41.0	35.0	41.0
42	38.28525291890594	40.0	38.0	41.0	34.0	41.0
43	38.307924373696466	40.0	38.0	41.0	34.0	41.0
44	38.282632622914385	40.0	38.0	41.0	34.0	41.0
45	38.30085971366776	40.0	38.0	41.0	34.0	41.0
46	38.26489797712523	40.0	38.0	41.0	34.0	41.0
47	38.19901110027042	40.0	38.0	41.0	34.0	41.0
48	38.14932832195806	40.0	38.0	41.0	34.0	41.0
49	38.01430956537223	40.0	38.0	41.0	33.0	41.0
50	37.84080972033958	40.0	38.0	41.0	33.0	41.0
51	37.739163666213194	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	14.0
12	43.0
13	84.0
14	124.0
15	333.0
16	498.0
17	842.0
18	1606.0
19	3126.0
20	5847.0
21	10707.0
22	18551.0
23	29459.0
24	44382.0
25	63875.0
26	88001.0
27	119901.0
28	158264.0
29	207870.0
30	269446.0
31	348495.0
32	451727.0
33	600630.0
34	847878.0
35	1251791.0
36	1904523.0
37	3235948.0
38	7330012.0
39	1.8759849E7
40	8535.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.31757517591131	29.091519472414745	28.792046278391386	23.798859073282557
2	20.18349621775647	28.625841708709928	31.20364331538188	19.987018758151727
3	19.46789072409882	27.362233415436794	32.124580813741474	21.04529504672291
4	20.324342050186647	27.251648809660832	31.744548322330175	20.679460817822346
5	24.62631393689434	27.16396999617363	28.393035562629905	19.81668050430212
6	22.205699890376398	26.921500061573095	30.189605432212378	20.683194615838122
7	24.316563789448868	27.55896616700928	27.810797896739064	20.31367214680279
8	21.435172141266246	27.249657824710283	30.494467760695514	20.820702273327953
9	21.167166987693097	27.16698154138098	30.88010493263702	20.785746538288905
10	21.42359852416948	27.334859108338787	30.557360680661787	20.684181686829945
11	24.0035811942228	27.22788913442319	28.0451699344775	20.723359736876514
12	21.33618601518339	29.928572129627533	27.91303462513713	20.822207230051944
13	22.90002109001308	27.492999217415665	28.526955145160642	21.080024547410613
14	22.537803141245778	27.62851971899403	28.66024099296322	21.173436146796973
15	22.612627596402163	27.604572761560668	28.449902595946376	21.33289704609079
16	22.55940748447579	27.577290666773745	28.445806906930194	21.417494941820273
17	22.56204891646261	27.594400589658903	28.377773797123385	21.4657766967551
18	22.40119992047507	27.612937845003	28.449171573360488	21.53669066116144
19	22.21117491871065	27.800451826853095	28.594509894366066	21.393863360070185
20	22.070426424734915	27.78047552326786	28.643941738540164	21.50515631345706
21	22.169642438706248	27.762079092516572	28.469174248285324	21.599104220491864
22	22.116591890633586	27.80632424882593	28.51546948117224	21.561614379368248
23	22.20325038842502	27.817973036016742	28.3890816861924	21.58969488936584
24	22.102650238077047	27.87058979197465	28.34294101073609	21.683818959212214
25	22.18123562944874	27.92462703536555	28.255374112209335	21.638763222976372
26	22.208205317029865	27.883223182175946	28.21537117280699	21.693200327987192
27	22.214277778630667	27.877410496167553	28.131313906666016	21.776997818535765
28	22.181750136707933	27.940439744880457	28.15514306489514	21.722667053516474
29	22.23903847508945	27.924499389861296	28.093965275222327	21.742496859826925
30	22.237347679287044	27.85594031626314	28.084331207113443	21.822380797336375
31	22.318667812862365	27.856064768427075	28.027384543912824	21.79788287479773
32	22.351855377407688	27.883043583600653	27.98521064955827	21.779890389433394
33	22.322023521877043	27.828526155014384	27.949667753305	21.899782569803573
34	22.29811582130153	27.88719814929308	27.96491307544125	21.84977295396414
35	22.36320288624026	27.854742401261767	27.909764557907163	21.87229015459081
36	22.36284479841053	27.831835958619816	27.87514246868695	21.930176774282707
37	22.365315194786987	27.834833179674895	27.883722763400115	21.916128862138002
38	22.37291697728317	27.837296529644195	27.92300431528117	21.866782177791464
39	22.441760498741335	27.884760440718892	27.763357919469044	21.910121141070732
40	22.454422206670184	27.823375362238277	27.844363902931267	21.877838528160268
41	22.383659078378496	27.77014714432037	27.91843207497929	21.927761702321856
42	22.433280540713348	27.816550683501735	27.78492208882498	21.965246686959933
43	22.39974786667603	27.871184724626385	27.780932582354378	21.948134826343203
44	22.442099761338262	27.789508463528286	27.825255574084363	21.94313620104909
45	22.43386987865346	27.69871360881767	27.816611269520937	22.050805243007932
46	22.335822690955734	27.81032253677133	27.829658502517674	22.02419626975526
47	22.393413419186157	27.81125469377127	27.795936881890775	21.999395005151793
48	22.35779711201865	27.725915673696168	27.79050565330102	22.125781560984162
49	22.444472512461644	27.795426545577996	27.7211444790249	22.03895646293546
50	22.466176341220248	27.995343002527424	27.560994883465728	21.977485772786594
51	22.3805922571321	27.722405247738756	27.792506854909544	22.1044956402196
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.116434249193865
2	40.1705149759082
3	40.51318577082173
4	41.00380286800899
5	44.44299444119646
6	42.88889450621452
7	44.63023593625166
8	42.2558744145942
9	41.952913525982
10	42.10778021099943
11	44.726940931099314
12	42.15839324523533
13	43.98004563742369
14	43.711239288042755
15	43.945524642492956
16	43.976902426296064
17	44.027825613217715
18	43.93789058163651
19	43.60503827878084
20	43.575582738191976
21	43.76874665919811
22	43.67820627000183
23	43.79294527779086
24	43.786469197289264
25	43.819998852425115
26	43.901405645017064
27	43.99127559716643
28	43.90441719022441
29	43.98153533491638
30	44.05972847662342
31	44.116550687660094
32	44.13174576684108
33	44.22180609168061
34	44.14788877526567
35	44.23549304083107
36	44.293021572693235
37	44.28144405692499
38	44.23969915507463
39	44.35188163981206
40	44.332260734830456
41	44.31142078070035
42	44.398527227673284
43	44.34788269301924
44	44.385235962387355
45	44.4846751216614
46	44.360018960710995
47	44.39280842433795
48	44.48357867300281
49	44.4834289753971
50	44.44366211400684
51	44.4850878973517
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26043.0
1	16251.0
2	6459.0
3	8444.0
4	10429.0
5	14952.0
6	19475.0
7	30934.0
8	42393.0
9	35286.5
10	28180.0
11	31649.0
12	35118.0
13	46604.5
14	58091.0
15	79216.0
16	100341.0
17	137354.0
18	174367.0
19	222005.0
20	269643.0
21	327467.0
22	385291.0
23	459790.0
24	534289.0
25	629426.0
26	836717.5
27	948872.0
28	1043856.5
29	1138841.0
30	1231965.5
31	1325090.0
32	1434251.0
33	1543412.0
34	1646606.5
35	1749801.0
36	1884231.0
37	2018661.0
38	2115676.0
39	2212691.0
40	2304093.5
41	2395496.0
42	2434362.0
43	2473228.0
44	2490075.0
45	2506922.0
46	2475640.0
47	2444358.0
48	2384961.0
49	2325564.0
50	2218654.0
51	2111744.0
52	1966202.5
53	1820661.0
54	1670566.5
55	1520472.0
56	1374001.5
57	1227531.0
58	1082566.5
59	937602.0
60	823512.5
61	709423.0
62	610683.0
63	511943.0
64	439268.0
65	366593.0
66	305746.5
67	244900.0
68	224904.0
69	204908.0
70	181069.5
71	157231.0
72	134816.0
73	112401.0
74	94927.0
75	64926.0
76	52399.0
77	49850.5
78	47302.0
79	45848.0
80	44394.0
81	38861.0
82	33328.0
83	29315.5
84	25303.0
85	22955.0
86	20607.0
87	24948.5
88	29290.0
89	18488.5
90	7687.0
91	4404.0
92	1121.0
93	743.5
94	366.0
95	214.0
96	62.0
97	44.0
98	26.0
99	16.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03148281103949145
2	0.0
3	0.007303766092472837
4	0.02983862479815377
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	2.7962351043157877E-6
13	0.003830842092912629
14	0.0
15	0.0
16	2.7962351043157877E-6
17	0.017076607782056514
18	5.5924702086315755E-6
19	0.0
20	0.0
21	0.0
22	6.151717229494733E-5
23	0.0
24	0.0
25	0.0
26	0.0
27	0.004275443474498839
28	0.0
29	3.355482125178945E-5
30	0.0
31	2.5166115938842087E-5
32	0.006476080501595364
33	2.516611593884209E-4
34	0.0025222040640928405
35	0.0011604375682910519
36	0.0028102162798373663
37	4.2782397096031554E-4
38	4.47397616690526E-4
39	9.702935811975782E-4
40	0.0
41	0.002278931610017367
42	0.0011995848597514728
43	9.507199354673678E-4
44	1.1464563927694728E-4
45	0.0
46	0.0
47	2.3488374876252615E-4
48	0.003934302791772313
49	1.0905316906831572E-4
50	7.661684185825259E-4
51	0.08375563007957078
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.5762372E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.768422058682983
#Duplication Level	Relative count
1	100.0
2	12.291984523970465
3	3.4866129769468963
4	1.681505372190785
5	1.016499610524215
6	0.7036314593432343
7	0.5040653546804689
8	0.429389005838918
9	0.3090055814133143
10++	8.329631703972654
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	10937470	3.4556062	4.2016783	16
TGTGT	5923390	3.095253	27.167248	4
TGTTT	5546070	2.2534707	21.118847	6
CTTTG	3845190	2.0675402	27.132006	1
GTTTG	3855900	2.014891	26.214273	7
GAGGG	2227230	1.9754335	8.091859	11
TTTGT	4656210	1.8919041	20.841423	2
TTTGA	4493480	1.8737109	21.175371	8
GTGTT	3522020	1.840423	26.216732	5
TTGTG	3412410	1.7831464	26.284231	3
TGAGG	2577435	1.7775664	10.221328	10
TTGAG	2928665	1.5705388	16.114656	9
TGAGT	2245025	1.2039269	5.0376573	10
TTGAT	2344490	0.97761565	5.1743875	9
>>END_MODULE