FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_18_CTTGTA_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_18_CTTGTA_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 28842441
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32565 0.11290653242560156 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 13805935 4.73674 6.165843 17
TGTGT 6437265 3.7986817 35.283005 4
AAAAA 8080220 3.7302437 3.9706059 45
GTTTG 4968680 2.9320579 34.777077 7
TGTTT 6275370 2.823657 27.137852 6
GTGTT 4547815 2.6837022 34.679607 5
GGGGG 1977570 2.6323283 5.5611587 13
TTTGA 5394395 2.5757008 28.309273 8
CTTTG 3969615 2.5132213 37.304142 1
TTGTG 4225765 2.4936578 34.69292 3
GAGGG 2094790 2.2561595 10.6581 11
TTTGT 4752020 2.1382124 26.81942 2
TTGAG 3355470 2.1011882 21.712708 9
TGAGG 2460345 2.0205355 13.240094 10
AGGGG 1585100 1.7072061 5.9497776 12
TGAGC 1710270 1.5069054 5.563806 10
AACTT 2739640 1.4892826 12.715404 2
ACTTT 2851375 1.4606894 11.625981 3
TGAGT 2036170 1.2750454 6.584356 10
GAGTG 1527050 1.2540755 5.23143 11
TTGAT 2384710 1.1386448 7.0603747 9
TAACT 2002380 1.0885041 12.459257 1
TTGAC 1466445 0.9852085 5.6442294 9
Produced by FastQC (version 0.10.1)