##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_18_CTTGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	28842441	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56097280393154	34.0	31.0	34.0	31.0	34.0
2	32.72837174218368	34.0	31.0	34.0	31.0	34.0
3	32.76509706650696	34.0	31.0	34.0	31.0	34.0
4	36.14176549758739	37.0	37.0	37.0	35.0	37.0
5	36.155967450882535	37.0	37.0	37.0	35.0	37.0
6	36.16810827488561	37.0	37.0	37.0	35.0	37.0
7	36.18967486143077	37.0	37.0	37.0	35.0	37.0
8	36.17859393384908	37.0	37.0	37.0	35.0	37.0
9	37.954647701281594	39.0	39.0	39.0	37.0	39.0
10	37.94263997974374	39.0	39.0	39.0	37.0	39.0
11	37.94313799584439	39.0	39.0	39.0	37.0	39.0
12	37.88484313792997	39.0	39.0	39.0	37.0	39.0
13	37.892617237216506	39.0	39.0	39.0	37.0	39.0
14	39.44568322077871	41.0	40.0	41.0	37.0	41.0
15	39.395479390943365	41.0	40.0	41.0	37.0	41.0
16	39.36403818941677	41.0	40.0	41.0	37.0	41.0
17	39.33553169095501	41.0	40.0	41.0	37.0	41.0
18	39.30720413019134	41.0	40.0	41.0	37.0	41.0
19	39.296582941783605	41.0	40.0	41.0	37.0	41.0
20	39.26534519044348	41.0	40.0	41.0	37.0	41.0
21	39.242698910262135	41.0	40.0	41.0	37.0	41.0
22	39.21224368630935	41.0	40.0	41.0	36.0	41.0
23	39.16853264950772	41.0	39.0	41.0	36.0	41.0
24	39.12451085537455	41.0	39.0	41.0	36.0	41.0
25	39.09163756285399	41.0	39.0	41.0	36.0	41.0
26	39.02559117655819	41.0	39.0	41.0	36.0	41.0
27	38.95831361846246	41.0	39.0	41.0	36.0	41.0
28	38.91998059387553	41.0	39.0	41.0	36.0	41.0
29	38.83067937973766	41.0	39.0	41.0	35.0	41.0
30	38.79531493190885	41.0	39.0	41.0	35.0	41.0
31	38.76147455757992	40.0	39.0	41.0	35.0	41.0
32	38.692713976601354	40.0	39.0	41.0	35.0	41.0
33	38.63690535069483	40.0	39.0	41.0	35.0	41.0
34	38.59451309963675	40.0	38.0	41.0	35.0	41.0
35	38.5463197099025	40.0	38.0	41.0	35.0	41.0
36	38.4667695428414	40.0	38.0	41.0	35.0	41.0
37	38.4078300446207	40.0	38.0	41.0	34.0	41.0
38	38.35349809678036	40.0	38.0	41.0	34.0	41.0
39	38.291521858361435	40.0	38.0	41.0	34.0	41.0
40	38.220167114149596	40.0	38.0	41.0	34.0	41.0
41	38.371683381444726	40.0	38.0	41.0	34.0	41.0
42	38.40760860011814	40.0	38.0	41.0	35.0	41.0
43	38.39501230149001	40.0	38.0	41.0	34.0	41.0
44	38.36364293854324	40.0	38.0	41.0	34.0	41.0
45	38.32487305772767	40.0	38.0	41.0	34.0	41.0
46	38.23090077570064	40.0	38.0	41.0	34.0	41.0
47	38.19828040906801	40.0	38.0	41.0	34.0	41.0
48	38.128003347566874	40.0	38.0	41.0	34.0	41.0
49	38.07387030799508	40.0	38.0	41.0	33.0	41.0
50	37.993325946302534	40.0	38.0	41.0	33.0	41.0
51	36.47635638745001	38.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	39.0
6	547.0
7	3219.0
8	12027.0
9	18792.0
10	18374.0
11	19039.0
12	22189.0
13	27080.0
14	31601.0
15	35143.0
16	37175.0
17	37822.0
18	38005.0
19	39623.0
20	41211.0
21	43830.0
22	47861.0
23	53853.0
24	59362.0
25	68177.0
26	79657.0
27	92421.0
28	110498.0
29	133779.0
30	163077.0
31	204715.0
32	264904.0
33	356716.0
34	514991.0
35	804372.0
36	1268277.0
37	2093190.0
38	5020253.0
39	1.7075398E7
40	5223.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.1812694105382	27.298537804755764	30.989048618210308	23.531144166495732
2	21.439761122484676	28.79114509492314	31.976963361604266	17.79213042098792
3	21.783365338820357	27.587354453163854	31.471267458893777	19.158012749122012
4	20.775847586407412	25.516492291784743	32.89569910350962	20.811961018298224
5	26.10280921740968	25.625951827552246	29.51829390059525	18.75294505444283
6	20.972179802879236	25.376759753610656	34.270718661699846	19.380341781810262
7	24.780300101070885	26.058167207511325	30.420629738575546	18.740902952842244
8	22.07474492219546	25.953234753410342	32.83197562293214	19.140044701462056
9	22.13226248075538	26.12883942721311	32.55483565955174	19.18406243247977
10	22.070125296258116	27.57990561069773	31.128099727738633	19.221869365305515
11	25.583781566483445	25.945756081754784	28.95249604216622	19.517966309595554
12	21.285128529498255	30.07399878489272	28.955534841397984	19.68533784421104
13	23.663769924321663	26.411060929514328	29.773303430582004	20.151865715582005
14	24.065905290868102	26.852491061385063	28.694786425718355	20.386817222028476
15	23.099197535388807	28.092232079597522	28.249498333928436	20.559072051085234
16	23.448694413402936	27.05555873949756	28.6654103124474	20.830336534652105
17	23.161700714793618	27.197596144311227	28.865885944518787	20.774817196376365
18	22.943981718981064	27.13472945642483	28.964356894817435	20.95693192977668
19	22.575033165812837	27.49778565193992	29.2134347216849	20.713746460562348
20	22.34323786385449	27.522297658212807	29.23188475844345	20.90257971948925
21	22.356797890164803	27.580574005318137	29.16673658861251	20.895891515904548
22	22.147545802512102	27.756733897288065	29.26379033526965	20.83192996493018
23	22.201222638735214	27.851219305098045	29.08144063468291	20.86611742148383
24	22.04517385278529	27.917413965075603	29.039050992157335	20.99836118998177
25	22.042666910580742	28.0316775319039	28.95927801062919	20.966377546886164
26	22.03929846062432	28.049508074005374	28.907659165714428	21.003534299655875
27	22.07325859338939	28.03098904706086	28.79287156465941	21.10288079489034
28	22.0369608247294	28.036455319472044	28.820109817376693	21.106474038421865
29	22.089820630898167	28.0028897127109	28.762504755945013	21.14478490044592
30	22.0919989809762	28.008472673045446	28.670515579913157	21.22901276606519
31	22.15351208393748	27.989943284925467	28.63075983221935	21.225784798917704
32	22.182520738817928	28.030005527669854	28.544868391975147	21.242605341537075
33	22.128965306753813	27.986719994551905	28.524157502886844	21.360157195807435
34	22.188698875089408	27.99872380085398	28.492061469102893	21.320515854953726
35	22.186709658098966	27.991801371482357	28.451275078287345	21.370213892131332
36	22.152031902327384	28.03355020467396	28.4456027467303	21.368815146268354
37	22.171307763425627	27.977154692625582	28.453521082119565	21.398016461829222
38	22.13078226847666	28.007365181264454	28.49300604035614	21.36884650990275
39	22.120340165091726	27.927444032808058	28.469044512664816	21.4831712894354
40	22.165269282640594	27.959392729801884	28.428793206933044	21.446544780624478
41	22.17243211301366	27.964944014737053	28.40665678093061	21.45596709131868
42	22.119585498416132	27.92307027822261	28.401794285655235	21.555549937706026
43	22.13634741748642	28.005055686803214	28.358645810860427	21.499951084849943
44	22.205716782277175	27.975727860775006	28.308374061405043	21.51018129554277
45	22.18143311687679	27.945714253713504	28.325032474723187	21.547820154686516
46	22.110664357874487	28.072547783754075	28.250836741786806	21.56595111658463
47	22.17993645714568	28.00573382397829	28.253828242315098	21.560501476560933
48	22.10981477423495	27.96355101448552	28.25646954992823	21.670164661351297
49	22.10784525337024	28.024599601148463	28.222964354919274	21.644590790562027
50	22.102194570860952	28.020270289959548	28.24221400038881	21.635321138790687
51	22.143145882499553	27.96454668954731	28.249660287706675	21.64264714024646
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.71241357703393
2	39.23189154347259
3	40.94137808794237
4	41.587808604705636
5	44.85575427185251
6	40.3525215846895
7	43.52120305391313
8	41.214789623657516
9	41.31632491323515
10	41.29199466156363
11	45.101747876078996
12	40.9704663737093
13	43.81563563990367
14	44.45272251289658
15	43.65826958647404
16	44.279030948055045
17	43.93651791116998
18	43.900913648757744
19	43.28877962637519
20	43.245817583343744
21	43.25268940606936
22	42.97947576744228
23	43.067340060219045
24	43.04353504276706
25	43.00904445746691
26	43.04283276028019
27	43.17613938827973
28	43.14343486315126
29	43.23460553134409
30	43.32101174704139
31	43.379296882855186
32	43.425126080355
33	43.48912250256125
34	43.509214730043134
35	43.556923550230294
36	43.52084704859573
37	43.56932422525485
38	43.4996287783794
39	43.60351145452713
40	43.61181406326507
41	43.62839920433234
42	43.675135436122154
43	43.63629850233636
44	43.71589807781995
45	43.72925327156331
46	43.67661547445912
47	43.740437933706616
48	43.77997943558625
49	43.75243604393227
50	43.73751570965164
51	43.78579302274601
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33841.0
1	21186.5
2	8532.0
3	11206.0
4	13880.0
5	35024.5
6	56169.0
7	69968.0
8	83767.0
9	60718.0
10	37669.0
11	36304.0
12	34939.0
13	41855.0
14	48771.0
15	64981.5
16	81192.0
17	118218.5
18	155245.0
19	192703.0
20	230161.0
21	274232.0
22	318303.0
23	379342.5
24	440382.0
25	518175.5
26	694494.5
27	793020.0
28	873714.0
29	954408.0
30	1024622.5
31	1094837.0
32	1183222.0
33	1271607.0
34	1354062.0
35	1436517.0
36	1562419.0
37	1688321.0
38	1762562.5
39	1836804.0
40	1908331.5
41	1979859.0
42	1999796.0
43	2019733.0
44	2024732.5
45	2029732.0
46	1985848.0
47	1941964.0
48	1882851.5
49	1823739.0
50	1736293.0
51	1648847.0
52	1532626.5
53	1416406.0
54	1297388.5
55	1178371.0
56	1057594.0
57	936817.0
58	829323.5
59	721830.0
60	630644.5
61	539459.0
62	461411.0
63	383363.0
64	326686.0
65	270009.0
66	226483.5
67	182958.0
68	162137.0
69	141316.0
70	122924.0
71	104532.0
72	89378.5
73	74225.0
74	63907.5
75	45117.0
76	36644.0
77	34737.0
78	32830.0
79	30695.5
80	28561.0
81	24781.0
82	21001.0
83	19203.5
84	17406.0
85	16026.0
86	14646.0
87	17101.0
88	19556.0
89	13083.5
90	6611.0
91	4280.5
92	1950.0
93	1523.5
94	1097.0
95	853.0
96	609.0
97	462.5
98	316.0
99	223.0
100	130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003983712751635689
2	7.350279402495787E-4
3	0.0053844263736207345
4	0.009555363223244524
5	0.013313713634709349
6	4.2298777693607835E-4
7	0.028506602475151115
8	0.007700457807992049
9	0.013389990119074872
10	0.003595396103956666
11	0.01847277766815922
12	0.019003245945792176
13	0.014107682494695923
14	0.006389889122075347
15	0.005349755244363679
16	0.05976262550038674
17	0.003845028234607466
18	9.881271838260847E-4
19	0.0034497773610770326
20	0.0021600113527145636
21	0.002624604484759109
22	0.0038796993638645218
23	2.4269790479938922E-4
24	0.0471388673378928
25	0.005838618166888163
26	0.01160442696233651
27	0.03846068368485178
28	3.8138242182761163E-5
29	0.09882658683431128
30	0.037881675826258954
31	7.280937143981677E-5
32	0.013480135055143219
33	0.010384003212488153
34	0.023631841701609098
35	0.015418251180612626
36	0.010106634178431707
37	0.017973513406857624
38	0.055456471246660435
39	0.019076055317231992
40	0.03217134083762189
41	0.021776936286356623
42	0.02005378116228096
43	0.023555565217243574
44	0.009676712175644218
45	0.013376121667372051
46	0.032840493632283065
47	0.020244472373194766
48	0.05190961472366364
49	0.007405753209307076
50	0.05621230186446424
51	0.12126574168947768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8842441E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.690326400015042
#Duplication Level	Relative count
1	100.0
2	14.860503190885094
3	4.270105917337699
4	1.935246750643189
5	1.177453306157907
6	0.8206087740987003
7	0.5913996458284607
8	0.48381168766079724
9	0.38090146680477127
10++	9.129606735941728
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32565	0.11290653242560156	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13805935	4.73674	6.165843	17
TGTGT	6437265	3.7986817	35.283005	4
AAAAA	8080220	3.7302437	3.9706059	45
GTTTG	4968680	2.9320579	34.777077	7
TGTTT	6275370	2.823657	27.137852	6
GTGTT	4547815	2.6837022	34.679607	5
GGGGG	1977570	2.6323283	5.5611587	13
TTTGA	5394395	2.5757008	28.309273	8
CTTTG	3969615	2.5132213	37.304142	1
TTGTG	4225765	2.4936578	34.69292	3
GAGGG	2094790	2.2561595	10.6581	11
TTTGT	4752020	2.1382124	26.81942	2
TTGAG	3355470	2.1011882	21.712708	9
TGAGG	2460345	2.0205355	13.240094	10
AGGGG	1585100	1.7072061	5.9497776	12
TGAGC	1710270	1.5069054	5.563806	10
AACTT	2739640	1.4892826	12.715404	2
ACTTT	2851375	1.4606894	11.625981	3
TGAGT	2036170	1.2750454	6.584356	10
GAGTG	1527050	1.2540755	5.23143	11
TTGAT	2384710	1.1386448	7.0603747	9
TAACT	2002380	1.0885041	12.459257	1
TTGAC	1466445	0.9852085	5.6442294	9
>>END_MODULE