FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_18_CTTGTA_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_18_CTTGTA_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 28842441
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37393 0.129645753630908 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12447790 4.4490943 5.617117 16
AAAAA 7851910 3.520836 3.7482657 45
TGTGT 5284815 3.258982 37.001495 4
TGTTT 5268855 2.4736145 28.33369 6
GTTTG 3943545 2.431862 36.32427 7
CTTTG 3526970 2.2778416 38.010525 1
GTGTT 3508740 2.1637313 36.268337 5
TTTGA 4399265 2.1611958 29.176056 8
GAGGG 1865135 2.0765073 11.029819 11
TTTGT 4266735 2.0031407 27.995132 2
TTGTG 3238635 1.997166 36.282413 3
TGAGG 2213125 1.8758268 14.002687 10
TTGAG 2774655 1.7904384 22.523243 9
AGGGG 1405990 1.5653281 6.147324 12
TGAGC 1643635 1.4590218 5.521914 10
TGAGT 1896040 1.2234828 6.7855687 10
GAGTG 1441410 1.2217275 5.4091654 11
TTGAT 2163650 1.0629212 7.3337665 9
TTGAC 1353160 0.91446936 5.616698 9
Produced by FastQC (version 0.10.1)