##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_18_CTTGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	28842441	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07693197673525	34.0	33.0	34.0	31.0	34.0
2	33.25032083796236	34.0	34.0	34.0	31.0	34.0
3	33.280638902927805	34.0	34.0	34.0	31.0	34.0
4	36.58493526952174	37.0	37.0	37.0	35.0	37.0
5	36.526829473275164	37.0	37.0	37.0	35.0	37.0
6	36.505912831719066	37.0	37.0	37.0	35.0	37.0
7	36.509132843506556	37.0	37.0	37.0	35.0	37.0
8	36.49200260130549	37.0	37.0	37.0	35.0	37.0
9	38.322079015434234	39.0	39.0	39.0	37.0	39.0
10	38.29410568959818	39.0	39.0	39.0	37.0	39.0
11	38.27773467578559	39.0	39.0	39.0	37.0	39.0
12	38.261405093972456	39.0	39.0	39.0	37.0	39.0
13	38.27413605526661	39.0	39.0	39.0	37.0	39.0
14	39.865473383476804	41.0	40.0	41.0	38.0	41.0
15	39.8427314109787	41.0	40.0	41.0	38.0	41.0
16	39.805817441041135	41.0	40.0	41.0	38.0	41.0
17	39.761700890711715	41.0	40.0	41.0	38.0	41.0
18	39.74951905076273	41.0	40.0	41.0	38.0	41.0
19	39.76420265538551	41.0	40.0	41.0	38.0	41.0
20	39.73997419289165	41.0	40.0	41.0	38.0	41.0
21	39.695510688571744	41.0	40.0	41.0	37.0	41.0
22	39.670909268740466	41.0	40.0	41.0	37.0	41.0
23	39.62987272817859	41.0	40.0	41.0	37.0	41.0
24	39.61542561532847	41.0	40.0	41.0	37.0	41.0
25	39.57191060909165	41.0	40.0	41.0	37.0	41.0
26	39.4886464359934	41.0	40.0	41.0	37.0	41.0
27	39.434952402260265	41.0	39.0	41.0	37.0	41.0
28	39.38975016018929	41.0	39.0	41.0	36.0	41.0
29	39.332264977156406	41.0	39.0	41.0	36.0	41.0
30	39.274655151413846	41.0	39.0	41.0	36.0	41.0
31	39.21055582639486	40.0	39.0	41.0	36.0	41.0
32	39.13981472649974	40.0	39.0	41.0	36.0	41.0
33	39.050078771072116	40.0	39.0	41.0	36.0	41.0
34	39.163788148166795	40.0	39.0	41.0	36.0	41.0
35	39.188845493347806	40.0	39.0	41.0	36.0	41.0
36	39.13734801433762	40.0	39.0	41.0	36.0	41.0
37	39.11938999892554	40.0	39.0	41.0	36.0	41.0
38	39.09451620270281	40.0	39.0	41.0	35.0	41.0
39	39.0668955862647	40.0	39.0	41.0	35.0	41.0
40	38.871996617762	40.0	39.0	41.0	35.0	41.0
41	38.893490082895546	40.0	39.0	41.0	35.0	41.0
42	38.855204419071185	40.0	39.0	41.0	35.0	41.0
43	38.612354966765814	40.0	38.0	41.0	35.0	41.0
44	38.68356274006073	40.0	38.0	41.0	35.0	41.0
45	38.69415411129731	40.0	38.0	41.0	35.0	41.0
46	38.665587423755156	40.0	38.0	41.0	35.0	41.0
47	38.621309444647906	40.0	38.0	41.0	35.0	41.0
48	38.56337433437066	40.0	38.0	41.0	35.0	41.0
49	38.51213411513956	40.0	38.0	41.0	35.0	41.0
50	38.562676820592266	40.0	38.0	41.0	35.0	41.0
51	38.284158195903046	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	10.0
11	15.0
12	47.0
13	92.0
14	179.0
15	262.0
16	498.0
17	770.0
18	1245.0
19	2280.0
20	3920.0
21	6886.0
22	11461.0
23	18455.0
24	27063.0
25	38407.0
26	52613.0
27	69845.0
28	91743.0
29	118899.0
30	154182.0
31	197748.0
32	256664.0
33	342649.0
34	492452.0
35	774257.0
36	1210013.0
37	2021497.0
38	4856475.0
39	1.8074048E7
40	17761.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.59837397110739	27.9529511945084	29.051585807197096	24.397089027187118
2	21.621607378293568	27.62014432050278	31.833295177612715	18.92495312359094
3	19.921329340533394	26.477408328516162	33.404872077657664	20.196390253292783
4	20.753910566038417	26.277548238512583	32.84925424059474	20.119286954854264
5	25.203213556023226	26.510048161318938	29.104707191738733	19.182031090919107
6	21.701264421895104	26.284286726187773	31.988455896399852	20.02599295551727
7	25.28527665186175	27.15793021818091	28.086426526797787	19.470366603159558
8	21.47222213265514	26.781987696533733	31.77872843702792	19.96706173378321
9	20.798329794624525	26.97877755908385	32.22292107661761	19.99997156967401
10	21.352745421235326	26.835037991409948	31.79845977668811	20.013756810666614
11	25.12440707108684	26.70363699124747	28.056935737552045	20.115020200113637
12	21.250278365829022	30.764604840484893	27.708892600317704	20.276224193368378
13	23.43620188823569	27.223348535734264	28.729431218109852	20.61101835792019
14	22.719138092368812	27.474512299427083	28.974905418026164	20.83144419017794
15	22.818106830833077	27.478509880630426	28.737675150310615	20.96570813822589
16	22.728277402040973	27.409105214083652	28.724312897094944	21.138304486780434
17	22.72526967237758	27.569399436919014	28.64161133125065	21.06371955945276
18	22.43736582489672	27.47395062713312	28.902404619636734	21.186278928333422
19	22.105493775648185	27.797806711297422	29.098795764200403	20.99790374885399
20	21.92289814780335	27.80949823133565	29.18281265028238	21.084790970578617
21	22.050779266567623	27.82651787343519	28.991499020488593	21.13120383950859
22	21.959299492022886	27.89604042182144	29.004809267010373	21.139850819145302
23	22.058691218264084	27.98095695159782	28.850474202235514	21.109877627902577
24	21.880949674127788	27.969512011830066	28.854249888211612	21.295288425830535
25	21.920464360142056	28.07161502037917	28.81913150138714	21.188789118091634
26	21.96224958383371	28.044974146948814	28.674262164462732	21.31851410475474
27	21.959814908850632	28.028968979668083	28.59936458908224	21.411851522399044
28	21.945805488516037	28.09259798780554	28.6526060675655	21.30899045611292
29	22.040357125112955	28.04986235388329	28.592618079724947	21.317162441278807
30	21.947923894513643	28.024084369280672	28.615032964789634	21.41295877141605
31	22.041119889956608	28.018627133535613	28.49989014452695	21.44036283198083
32	22.05303635708226	28.098811054168404	28.44662835576226	21.401524232987075
33	22.047149199334413	28.042737436820968	28.38942445960104	21.520688904243578
34	22.03938963802886	28.04158931992411	28.389127409471154	21.529893632575874
35	22.070370940784127	28.021592289733967	28.41250536284598	21.49553140663593
36	22.09926205175283	28.06699663918421	28.359122100856926	21.47461920820603
37	22.17856318055743	28.027589620448563	28.302035878308633	21.491811320685375
38	22.06014740569288	28.0424184624318	28.410844283256054	21.48658984861926
39	22.03916443826651	28.025145305835935	28.357679573653282	21.578010682244265
40	22.10540016359919	28.19431267970696	28.221480976592794	21.478806180101053
41	22.083380632596818	27.970906324158285	28.369964171371343	21.57574887187356
42	22.03811319369085	28.008930093657664	28.269033443607817	21.683923269043675
43	22.175719741763768	28.08477150398018	28.185258883198046	21.554249871058012
44	22.101528931031112	28.005769553677485	28.229145253631287	21.66355626166012
45	22.13939867294866	27.96628759680916	28.24410388843302	21.650209841809158
46	22.02908207387856	28.045601272097603	28.26923699003146	21.65607966399238
47	22.098384056703907	28.074540346814132	28.20308724056355	21.62398835591841
48	22.036293668764024	27.997706574141905	28.24748085642266	21.71851890067141
49	22.1243925788772	28.02996682526173	28.16923041291808	21.67641018294299
50	22.038484102257048	28.028186385544846	28.158684048185556	21.77464546401255
51	22.087640812245134	27.970623210822993	28.161594908625027	21.78014106830685
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.99546299829451
2	40.54656050188451
3	40.11771959382617
4	40.87319752089268
5	44.385244646942326
6	41.72725737741237
7	44.755643255021305
8	41.439283866438345
9	40.79830136429853
10	41.36650223190194
11	45.23942727120048
12	41.5265025591974
13	44.04722024615588
14	43.55058228254675
15	43.783814969058966
16	43.86658188882141
17	43.788989231830335
18	43.623644753230145
19	43.10339752450217
20	43.00768911838196
21	43.18198310607622
22	43.09915031116818
23	43.16856884616666
24	43.17623809995832
25	43.10925347823369
26	43.28076368858845
27	43.37166643124967
28	43.25479594462896
29	43.35751956639176
30	43.360882665929694
31	43.48148272193744
32	43.454560590069335
33	43.56783810357799
34	43.569283270604735
35	43.56590234742006
36	43.57388125995886
37	43.670374501242804
38	43.54673725431215
39	43.61717512051078
40	43.584206343700245
41	43.65912950447038
42	43.72203646273452
43	43.729969612821776
44	43.76508519269123
45	43.789608514757816
46	43.685161737870935
47	43.72237241262232
48	43.75481256943544
49	43.800802761820194
50	43.8131295662696
51	43.86778188055198
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47938.0
1	28844.5
2	9751.0
3	13232.5
4	16714.0
5	25423.0
6	34132.0
7	53288.5
8	72445.0
9	53172.0
10	33899.0
11	33205.0
12	32511.0
13	39777.0
14	47043.0
15	64328.5
16	81614.0
17	119849.5
18	158085.0
19	197141.5
20	236198.0
21	278560.5
22	320923.0
23	381861.0
24	442799.0
25	521640.5
26	700316.5
27	800151.0
28	874792.0
29	949433.0
30	1017126.0
31	1084819.0
32	1170756.5
33	1256694.0
34	1338082.5
35	1419471.0
36	1545286.0
37	1671101.0
38	1744489.5
39	1817878.0
40	1887553.0
41	1957228.0
42	1978160.0
43	1999092.0
44	2010604.5
45	2022117.0
46	1980254.0
47	1938391.0
48	1884195.0
49	1829999.0
50	1752971.0
51	1675943.0
52	1556634.0
53	1437325.0
54	1318608.0
55	1199891.0
56	1077639.0
57	955387.0
58	846353.0
59	737319.0
60	644281.0
61	551243.0
62	470673.0
63	390103.0
64	333167.0
65	276231.0
66	230789.0
67	185347.0
68	164709.5
69	144072.0
70	125143.0
71	106214.0
72	90782.5
73	75351.0
74	64346.5
75	44264.0
76	35186.0
77	33828.5
78	32471.0
79	30374.5
80	28278.0
81	24563.5
82	20849.0
83	18804.0
84	16759.0
85	15722.0
86	14685.0
87	17133.5
88	19582.0
89	12221.0
90	4860.0
91	2803.0
92	746.0
93	508.0
94	270.0
95	165.5
96	61.0
97	38.0
98	15.0
99	9.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03378008123514927
2	3.744481959762005E-4
3	0.004136265720366733
4	0.050775868796957925
5	0.0
6	3.8138242182761163E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0054627484546125E-4
12	0.0
13	2.322965660222725E-4
14	0.0
15	0.0
16	0.0
17	0.016194884475970672
18	0.0
19	0.0
20	0.0031308029719121205
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.386845170282224E-5
27	1.0401338777116682E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0034601786998541485
35	0.0016607470914129633
36	1.3521740410251683E-4
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0043650951734633
42	0.00687181781874842
43	4.507246803417228E-5
44	6.93422585141112E-6
45	0.0
46	0.0
47	0.008258662989030644
48	0.0
49	4.507246803417228E-5
50	0.009860469160706613
51	0.1414270033524555
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8842441E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.549441305536476
#Duplication Level	Relative count
1	100.0
2	15.112609429988359
3	4.338844365491115
4	2.0624314485468775
5	1.215926142748518
6	0.8586174643868892
7	0.6002247478315872
8	0.4817947527437404
9	0.39364515412721807
10++	9.515446366688872
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37393	0.129645753630908	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	12447790	4.4490943	5.617117	16
AAAAA	7851910	3.520836	3.7482657	45
TGTGT	5284815	3.258982	37.001495	4
TGTTT	5268855	2.4736145	28.33369	6
GTTTG	3943545	2.431862	36.32427	7
CTTTG	3526970	2.2778416	38.010525	1
GTGTT	3508740	2.1637313	36.268337	5
TTTGA	4399265	2.1611958	29.176056	8
GAGGG	1865135	2.0765073	11.029819	11
TTTGT	4266735	2.0031407	27.995132	2
TTGTG	3238635	1.997166	36.282413	3
TGAGG	2213125	1.8758268	14.002687	10
TTGAG	2774655	1.7904384	22.523243	9
AGGGG	1405990	1.5653281	6.147324	12
TGAGC	1643635	1.4590218	5.521914	10
TGAGT	1896040	1.2234828	6.7855687	10
GAGTG	1441410	1.2217275	5.4091654	11
TTGAT	2163650	1.0629212	7.3337665	9
TTGAC	1353160	0.91446936	5.616698	9
>>END_MODULE