FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_C_17_CAGATC_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_17_CAGATC_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 45160315
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 8107525 3.3358748 21.935785 4
TTTTT 12196610 3.079676 3.6918783 16
TGTTT 7497360 2.4165843 17.371939 6
GTTTG 5316140 2.1873474 21.228823 7
CTTTG 4921595 2.1113474 22.290337 1
GTGTT 4902320 2.0170798 21.144596 5
GAGGG 2938455 2.0120118 6.766924 11
TTTGA 6102815 2.0089004 17.222536 8
TTGTG 4705560 1.9361222 21.193909 3
TTTGT 5984995 1.929112 17.034336 2
TGAGG 3346005 1.7947758 8.3294525 10
TTGAG 3981635 1.6730825 13.247025 9
ACTTT 3905375 1.3403658 6.585548 3
AACTT 3601425 1.2623202 6.88298 2
TAACT 2681580 0.93990916 6.6448364 1
Produced by FastQC (version 0.10.1)