##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_17_CAGATC_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	45160315	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22376283247803	33.0	31.0	34.0	31.0	34.0
2	32.38732398124326	34.0	31.0	34.0	31.0	34.0
3	32.46866812155761	34.0	31.0	34.0	31.0	34.0
4	35.94469580205541	37.0	37.0	37.0	35.0	37.0
5	35.95804356103362	37.0	37.0	37.0	35.0	37.0
6	35.97287510062762	37.0	37.0	37.0	35.0	37.0
7	35.98972852602999	37.0	37.0	37.0	35.0	37.0
8	35.98663109413652	37.0	37.0	37.0	35.0	37.0
9	37.72055064274906	39.0	38.0	39.0	35.0	39.0
10	37.705408786453326	39.0	38.0	39.0	35.0	39.0
11	37.69883983315883	39.0	38.0	39.0	35.0	39.0
12	37.64344276606574	39.0	38.0	39.0	35.0	39.0
13	37.61450698472763	39.0	38.0	39.0	35.0	39.0
14	39.11788361086498	41.0	39.0	41.0	36.0	41.0
15	39.08162144130306	41.0	39.0	41.0	36.0	41.0
16	39.047024406273515	41.0	39.0	41.0	36.0	41.0
17	39.032776985722975	41.0	39.0	41.0	36.0	41.0
18	39.0076773158026	41.0	39.0	41.0	36.0	41.0
19	39.011681805142416	41.0	39.0	41.0	36.0	41.0
20	38.98868050411075	41.0	39.0	41.0	36.0	41.0
21	38.94153477450279	41.0	39.0	41.0	36.0	41.0
22	38.90427489267956	41.0	39.0	41.0	36.0	41.0
23	38.876302324286264	41.0	39.0	41.0	35.0	41.0
24	38.83795146247319	41.0	39.0	41.0	35.0	41.0
25	38.76840489265852	41.0	39.0	41.0	35.0	41.0
26	38.69991427207715	40.0	39.0	41.0	35.0	41.0
27	38.6694212163932	40.0	39.0	41.0	35.0	41.0
28	38.6309725253245	40.0	39.0	41.0	35.0	41.0
29	38.54982054487441	40.0	38.0	41.0	34.0	41.0
30	38.51099446494118	40.0	38.0	41.0	34.0	41.0
31	38.451045768834874	40.0	38.0	41.0	34.0	41.0
32	38.40178563856342	40.0	38.0	41.0	34.0	41.0
33	38.33239256634946	40.0	38.0	41.0	34.0	41.0
34	38.27909679106534	40.0	38.0	41.0	34.0	41.0
35	38.22404589073393	40.0	38.0	41.0	34.0	41.0
36	38.17344743941666	40.0	38.0	41.0	34.0	41.0
37	38.10687179662055	40.0	38.0	41.0	33.0	41.0
38	38.052267483076676	40.0	38.0	41.0	33.0	41.0
39	37.963659221597545	40.0	38.0	41.0	33.0	41.0
40	37.88853239841219	40.0	38.0	41.0	33.0	41.0
41	38.06654539500001	40.0	38.0	41.0	33.0	41.0
42	38.11053709434932	40.0	38.0	41.0	33.0	41.0
43	38.092542335012496	40.0	38.0	41.0	33.0	41.0
44	38.0603675151513	40.0	38.0	41.0	33.0	41.0
45	37.99918178161512	40.0	38.0	41.0	33.0	41.0
46	37.95853036011817	40.0	38.0	41.0	33.0	41.0
47	37.90986170047751	40.0	38.0	41.0	33.0	41.0
48	37.84960490200301	40.0	38.0	41.0	33.0	41.0
49	37.783997033678794	40.0	38.0	41.0	33.0	41.0
50	37.708029583053175	40.0	37.0	41.0	33.0	41.0
51	36.216520766075256	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	14.0
6	499.0
7	3339.0
8	10823.0
9	17574.0
10	20897.0
11	25368.0
12	33959.0
13	45320.0
14	56024.0
15	62860.0
16	66699.0
17	69290.0
18	71206.0
19	74089.0
20	79075.0
21	84867.0
22	93555.0
23	104078.0
24	118924.0
25	137108.0
26	158653.0
27	185202.0
28	219771.0
29	265136.0
30	326449.0
31	411957.0
32	533005.0
33	714147.0
34	1018671.0
35	1493736.0
36	2266461.0
37	3800500.0
38	8751630.0
39	2.3835292E7
40	4136.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.18618444410954	28.574934496644637	29.140048328307603	23.098832730938216
2	22.200384944122252	29.437806554572955	29.711365869595806	18.65044263170899
3	21.780069514572695	27.946375041892423	29.91962079981063	20.35393464372425
4	21.542100039120722	26.72171588010307	30.428183891040927	21.308000189735278
5	24.46486026272612	26.98302616812553	28.66492681704565	19.8871867521027
6	21.806654898366258	26.749473939787233	30.83932261696512	20.60454854488139
7	23.730238341814673	27.234381282361237	28.93599729020057	20.09938308562352
8	21.82788445909347	27.06415169715488	30.623065929038333	20.48489791471331
9	21.817683208328077	26.998803710821797	30.72424416063255	20.45926892021758
10	21.910425411366514	27.839504361605034	29.82342649657756	20.426643730450895
11	23.82564276871888	27.045070706480512	28.55345362261808	20.575832902182526
12	21.50622486717892	29.302669086799238	28.533359924756667	20.657746121265173
13	22.850685146353403	27.382538825594878	28.89711273275946	20.86966329529226
14	23.05373569820973	27.59456635781536	28.374508278636107	20.9771896653388
15	22.555867703181416	28.161360531009787	28.14332447643847	21.139447289370327
16	22.77415475008456	27.651790035406105	28.314413148708855	21.259642065800477
17	22.662231853791567	27.673223483102564	28.396734542215107	21.26781012089076
18	22.542754356746432	27.641257733932957	28.41415280169148	21.401835107629136
19	22.373888247050715	27.81604533825482	28.531091768838962	21.278974645855506
20	22.242798038158394	27.840445220621262	28.525368340209912	21.39138840101043
21	22.286381048066552	27.849520781799676	28.43355121736135	21.430546952772424
22	22.19258576145421	27.91468021358184	28.486535084324387	21.406198940639563
23	22.21413563462357	27.960466455295908	28.397556270478102	21.42784163960242
24	22.157072821289887	27.969842008390028	28.335714098405273	21.537371071914816
25	22.18284293513475	28.07732269466639	28.26152262672797	21.47831174347089
26	22.205340885779005	28.04630913014718	28.228395048605847	21.519954935467972
27	22.190869867835573	28.02880994440905	28.166597020193933	21.613723167561442
28	22.179102887896583	28.03750956190239	28.152966523127876	21.63042102707315
29	22.200458408617344	28.059456990868785	28.116184215319112	21.62390038519476
30	22.228502745395684	28.020036564234	28.072745716562014	21.6787149738083
31	22.279534882814588	28.02649776279339	28.001597346742756	21.692370007649266
32	22.31434742320832	28.03492025754763	27.964296530366916	21.68643578887713
33	22.269841480399435	28.007585370910938	27.949072487081246	21.77350066160838
34	22.29359395595347	28.029661773326286	27.92018682519683	21.756557445523416
35	22.318294539335373	28.005344103689094	27.903065380781673	21.77329597619386
36	22.320048389604615	27.994005468213118	27.88663787718757	21.799308264994696
37	22.305263007062244	27.992791960066892	27.8916024753486	21.810342557522258
38	22.30727102353047	27.998751386394787	27.925914854439675	21.768062735635063
39	22.30291990938193	27.936281731678005	27.906680939718946	21.854117419221122
40	22.31396899268004	27.949287625080725	27.902736379574666	21.83400700266457
41	22.322977155637606	27.964156057685525	27.891974068971066	21.82089271770581
42	22.274729185486493	27.951210632890387	27.874854608069597	21.89920557355352
43	22.312732552549676	27.986646549847453	27.83370840299791	21.86691249460496
44	22.357530584239033	27.945074485178495	27.829881892655955	21.867513037926518
45	22.3507935975719	27.938188590803286	27.799454462433598	21.911563349191216
46	22.287517693347443	27.98940047106655	27.783435110689297	21.93964672489671
47	22.308857630744903	27.95076288623261	27.80947888081965	21.930900602202836
48	22.279214780099053	27.893147516489307	27.778866276039054	22.048771427372582
49	22.320825345301447	27.957424693437744	27.764748569750395	21.95700139151041
50	22.278535397825348	27.951366931649996	27.80428172278308	21.965815947741575
51	22.30753263483463	27.905950146382843	27.798208562606607	21.988308656175924
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.285017175047756
2	40.85082757583125
3	42.134004158296946
4	42.850100228856
5	44.35204701482882
6	42.41120344324764
7	43.82962142743819
8	42.31278237380678
9	42.27695212854565
10	42.33706914181741
11	44.401475670901405
12	42.16397098844409
13	43.72034844164566
14	44.03092536354853
15	43.695314992551744
16	44.03379681588504
17	43.930041974682325
18	43.944589464375575
19	43.65286289290622
20	43.63418643916883
21	43.716928000838976
22	43.59878470209377
23	43.641977274225994
24	43.694443893204706
25	43.66115467860564
26	43.725295821246974
27	43.80459303539702
28	43.80952391496973
29	43.8243587938121
30	43.907217719203985
31	43.971904890463854
32	44.00078321208545
33	44.043342142007816
34	44.05015140147689
35	44.091590515529234
36	44.119356654599315
37	44.115605564584506
38	44.07533375916554
39	44.15703732860305
40	44.14797599534461
41	44.14386987334341
42	44.17393475904001
43	44.17964504715464
44	44.22504362216555
45	44.26235694676311
46	44.22716441824415
47	44.23975823294774
48	44.32798620747164
49	44.27782673681186
50	44.244351345566926
51	44.295841291010554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10840.0
1	7786.5
2	4733.0
3	6508.0
4	8283.0
5	14854.0
6	21425.0
7	28188.5
8	34952.0
9	31671.5
10	28391.0
11	34270.0
12	40149.0
13	53459.0
14	66769.0
15	90903.0
16	115037.0
17	161620.5
18	208204.0
19	265650.5
20	323097.0
21	393211.5
22	463326.0
23	556475.5
24	649625.0
25	770827.5
26	1038951.0
27	1185872.0
28	1312377.5
29	1438883.0
30	1561967.5
31	1685052.0
32	1828029.0
33	1971006.0
34	2110644.5
35	2250283.0
36	2434031.5
37	2617780.0
38	2742952.5
39	2868125.0
40	2984664.0
41	3101203.0
42	3144581.5
43	3187960.0
44	3203584.0
45	3219208.0
46	3176838.0
47	3134468.0
48	3048415.5
49	2962363.0
50	2814877.5
51	2667392.0
52	2477792.0
53	2288192.0
54	2094399.5
55	1900607.0
56	1712212.5
57	1523818.0
58	1336834.5
59	1149851.0
60	1006905.0
61	863959.0
62	738873.5
63	613788.0
64	521960.5
65	430133.0
66	357574.0
67	285015.0
68	261487.5
69	237960.0
70	210154.0
71	182348.0
72	153968.0
73	125588.0
74	106437.0
75	73869.0
76	60452.0
77	56220.0
78	51988.0
79	51837.0
80	51686.0
81	45568.5
82	39451.0
83	35720.5
84	31990.0
85	28862.0
86	25734.0
87	29483.5
88	33233.0
89	22909.0
90	12585.0
91	8353.5
92	4122.0
93	3118.0
94	2114.0
95	1653.5
96	1193.0
97	901.5
98	610.0
99	383.0
100	156.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.3073006687398E-5
2	0.003438859981379669
3	0.0
4	0.005892341539247456
5	0.013775368927342513
6	0.007555306024769756
7	0.025655268347884643
8	0.006587642269545728
9	0.012579628817912365
10	0.007085867315141623
11	0.02605384838436136
12	0.028622475286100197
13	0.012039331435132816
14	0.014260307971722518
15	0.0017072511562419349
16	0.041332749782635486
17	0.00210361685918267
18	5.2922571509964E-4
19	0.0059521285447189645
20	0.0011115954350628423
21	0.001131524436886678
22	0.01682893487346136
23	0.0010828090990950795
24	0.02117567160459355
25	0.00393044202636762
26	0.00434673673113219
27	0.008159819080092777
28	0.0013950301276685073
29	0.05930649509419941
30	0.006802432622535959
31	9.455204198642104E-4
32	0.005949914211182982
33	0.009849355568046857
34	0.016649573857046834
35	0.013841798933421967
36	0.011430389712737831
37	0.01639049683333697
38	0.027991390228345396
39	0.021027311257682767
40	0.022304981707944243
41	0.02642364208487031
42	0.0163019234918977
43	0.022223051367112917
44	0.01602734613343596
45	0.01399680228094069
46	0.029678712382763497
47	0.020847950241268245
48	0.047991250725332626
49	0.012807705172118485
50	0.04538055148641014
51	0.09749710558927678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.5160315E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.759912432396177
#Duplication Level	Relative count
1	100.0
2	11.065423806600059
3	2.7337959948152144
4	1.1740190150147916
5	0.7070162055556589
6	0.48126717150104503
7	0.3504071545078485
8	0.2722632581043283
9	0.23009036163258725
10++	7.023647831506874
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	8107525	3.3358748	21.935785	4
TTTTT	12196610	3.079676	3.6918783	16
TGTTT	7497360	2.4165843	17.371939	6
GTTTG	5316140	2.1873474	21.228823	7
CTTTG	4921595	2.1113474	22.290337	1
GTGTT	4902320	2.0170798	21.144596	5
GAGGG	2938455	2.0120118	6.766924	11
TTTGA	6102815	2.0089004	17.222536	8
TTGTG	4705560	1.9361222	21.193909	3
TTTGT	5984995	1.929112	17.034336	2
TGAGG	3346005	1.7947758	8.3294525	10
TTGAG	3981635	1.6730825	13.247025	9
ACTTT	3905375	1.3403658	6.585548	3
AACTT	3601425	1.2623202	6.88298	2
TAACT	2681580	0.93990916	6.6448364	1
>>END_MODULE