FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_C_17_CAGATC_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_17_CAGATC_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 45160315
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 7213105 3.0147314 23.107403 4
TGTTT 6767465 2.173141 17.821524 6
CTTTG 4618525 1.9781952 22.888847 1
GAGGG 2688210 1.942531 7.2091284 11
GTTTG 4524815 1.8911552 22.173155 7
TTTGT 5709330 1.8333569 17.55329 2
TTTGA 5376785 1.7621132 17.660805 8
TGAGG 3122675 1.7336723 8.930676 10
GTGTT 4103300 1.7149825 22.141533 5
TTGTG 4025605 1.6825094 22.226051 3
TTGAG 3536805 1.5086441 13.81594 9