##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_17_CAGATC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	45160315	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49703977928409	34.0	31.0	34.0	31.0	34.0
2	32.800669415171264	34.0	31.0	34.0	31.0	34.0
3	32.922835392091486	34.0	31.0	34.0	31.0	34.0
4	36.39679988946047	37.0	37.0	37.0	35.0	37.0
5	36.308843505630996	37.0	37.0	37.0	35.0	37.0
6	36.29824313670088	37.0	37.0	37.0	35.0	37.0
7	36.293494719866324	37.0	37.0	37.0	35.0	37.0
8	36.29688145443627	37.0	37.0	37.0	35.0	37.0
9	38.04702369768678	39.0	38.0	39.0	37.0	39.0
10	38.039352803451436	39.0	38.0	39.0	37.0	39.0
11	38.03513502950544	39.0	38.0	39.0	35.0	39.0
12	38.02110740370168	39.0	38.0	39.0	35.0	39.0
13	37.998606874199176	39.0	38.0	39.0	35.0	39.0
14	39.50251575968857	41.0	39.0	41.0	37.0	41.0
15	39.52566606322387	41.0	39.0	41.0	37.0	41.0
16	39.506834573673814	41.0	39.0	41.0	37.0	41.0
17	39.494025141321536	41.0	39.0	41.0	37.0	41.0
18	39.46774396945637	41.0	39.0	41.0	37.0	41.0
19	39.488177529319714	41.0	39.0	41.0	37.0	41.0
20	39.46208264933493	41.0	39.0	41.0	37.0	41.0
21	39.43492466781952	41.0	39.0	41.0	37.0	41.0
22	39.41404671335884	41.0	39.0	41.0	37.0	41.0
23	39.382609266565126	41.0	39.0	41.0	36.0	41.0
24	39.340764961449004	41.0	39.0	41.0	36.0	41.0
25	39.29901945989526	41.0	39.0	41.0	36.0	41.0
26	39.22028748027998	40.0	39.0	41.0	36.0	41.0
27	39.17235203075975	40.0	39.0	41.0	36.0	41.0
28	39.10694438247386	40.0	39.0	41.0	36.0	41.0
29	39.055737388014236	40.0	39.0	41.0	36.0	41.0
30	38.99734220188677	40.0	39.0	41.0	36.0	41.0
31	38.931600787107	40.0	39.0	41.0	35.0	41.0
32	38.84954589001428	40.0	38.0	41.0	35.0	41.0
33	38.77798979037237	40.0	38.0	41.0	35.0	41.0
34	38.911587175598754	40.0	39.0	41.0	35.0	41.0
35	38.932434682973316	40.0	39.0	41.0	35.0	41.0
36	38.93471834729231	40.0	39.0	41.0	35.0	41.0
37	38.8957220736835	40.0	38.0	41.0	35.0	41.0
38	38.858225125311904	40.0	38.0	41.0	35.0	41.0
39	38.62431296150171	40.0	38.0	41.0	35.0	41.0
40	38.5696390514548	40.0	38.0	41.0	35.0	41.0
41	38.62733413174819	40.0	38.0	41.0	35.0	41.0
42	38.39363354750736	40.0	38.0	41.0	34.0	41.0
43	38.41883137883338	40.0	38.0	41.0	34.0	41.0
44	38.397873819082086	40.0	38.0	41.0	34.0	41.0
45	38.4178058766862	40.0	38.0	41.0	34.0	41.0
46	38.38428412202173	40.0	38.0	41.0	34.0	41.0
47	38.32183037252951	40.0	38.0	41.0	34.0	41.0
48	38.27507316988378	40.0	38.0	41.0	34.0	41.0
49	38.144923214109554	40.0	38.0	41.0	34.0	41.0
50	37.968466163267465	40.0	38.0	41.0	33.0	41.0
51	37.87508218222127	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	5.0
11	14.0
12	45.0
13	77.0
14	147.0
15	321.0
16	470.0
17	889.0
18	1701.0
19	3393.0
20	6504.0
21	12022.0
22	20912.0
23	33576.0
24	50738.0
25	73060.0
26	103030.0
27	139457.0
28	186594.0
29	244762.0
30	319750.0
31	416210.0
32	539246.0
33	718980.0
34	1013090.0
35	1490782.0
36	2278773.0
37	3921827.0
38	9033137.0
39	2.4537617E7
40	13179.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.867114347515248	29.418222077191057	28.809630173775435	23.90503340151826
2	20.469040129591658	28.912947130683214	30.953382411083712	19.66463032864142
3	19.23922016267711	27.635019366518087	31.92968346322111	21.196077007583686
4	20.085555865263967	27.56812457815027	31.493087999381924	20.85323155720384
5	23.95932357867743	27.443107958835096	28.75094870352432	19.84661975896315
6	21.977331203292096	27.310119515331106	30.132398323616655	20.58015095776015
7	23.81719658067044	27.970256629077987	28.00208988799126	20.210456902260315
8	21.289661066358814	27.51395777465237	30.471536790653474	20.72484436833534
9	21.012579296667884	27.43540207812988	30.856418959876606	20.695599665325627
10	21.305916488846457	27.603680355196815	30.414110264731324	20.676292891225405
11	23.450755381134964	27.529214532715287	28.296164453237317	20.723865632912435
12	21.278959192379414	29.782980920305803	28.13127632081397	20.80678356650081
13	22.596584368643732	27.854743049503284	28.571874301283263	20.97679828056972
14	22.25447940298911	27.96095642822686	28.707087184843594	21.077476983940436
15	22.286330376570668	27.930006688394442	28.552706065048483	21.230956869986404
16	22.27703239005308	27.88071562388349	28.54980750244988	21.292444483613547
17	22.29956641364089	27.875227073846638	28.504219192858226	21.320987319654243
18	22.163418132199393	27.88375049570626	28.560681144969035	21.39215022712531
19	22.00359319902884	28.034264154269074	28.663812464549903	21.298330182152185
20	21.894092191341002	28.022437398853395	28.70112841329827	21.382341996507332
21	21.99036698481842	27.990958433305877	28.55642614538893	21.46224843648677
22	21.946429257477007	28.03453742597063	28.577846663279473	21.441186653272894
23	22.03171966360288	28.032275682753767	28.46889575504511	21.46710889859825
24	21.943629046874452	28.065532758130672	28.418741100455126	21.572097094539753
25	21.99231116966301	28.11460017495449	28.37410899370387	21.51897966167862
26	22.056349695523604	28.079463130405536	28.316259530076348	21.54792764399451
27	22.05329433046402	28.06836745519719	28.24036651264803	21.637971701690763
28	22.032011955629628	28.116841080492904	28.257530976035046	21.593615987842423
29	22.07515697857127	28.10570097136636	28.22149735750229	21.597644692560085
30	22.06726857418953	28.045579398637944	28.21060481974052	21.676547207432012
31	22.14169501291012	28.052031535216603	28.138979575093657	21.66729387677962
32	22.18039791380455	28.07964352612012	28.082528987817685	21.65742957225765
33	22.133332784177473	28.032938545628465	28.063365766079844	21.770362904114222
34	22.133501744993847	28.05418654034611	28.06899858799489	21.74331312666515
35	22.188064529650898	28.056607565985246	28.018330184554063	21.73699771980979
36	22.20794380468365	28.02797781513019	28.007118216306775	21.756960163879384
37	22.190321424829982	28.04372900623712	27.991215881775737	21.774733687157163
38	22.183759837768438	28.025061481208873	28.07470924580836	21.71646943521433
39	22.278766765381146	28.085098288803906	27.888498980311592	21.74763596550336
40	22.259273435094507	28.018921037198258	27.966483404732674	21.75532212297456
41	22.208339266958852	27.966195130645644	28.042290210344966	21.783175392050534
42	22.279563301777973	28.005114727061397	27.904912755351607	21.81040921580902
43	22.214180426496544	28.055602075443176	27.919412659422143	21.81080483863814
44	22.262026877254137	28.005580037744682	27.918891003924067	21.81350208107711
45	22.260030737163813	27.91483186067236	27.941368433767565	21.883768968396254
46	22.189701289727495	27.980393848005708	27.963370051781084	21.86653481048571
47	22.22655937224758	28.015224193750676	27.9216440556185	21.83657237838324
48	22.208220692259943	27.921292967797882	27.916321595484106	21.954164744458065
49	22.2809203248581	27.989125663129876	27.83335379010458	21.896600221907438
50	22.30977219523496	28.190015382201405	27.695923112110798	21.804289310452845
51	22.232149664109443	27.893190969162607	27.90568824320994	21.968971123518013
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.772147749033515
2	40.13367045823307
3	40.435297170260796
4	40.93878742246781
5	43.80594333764058
6	42.55748216105224
7	44.02765348293076
8	42.01450543469416
9	41.70817896199351
10	41.98220938007186
11	44.174621014047396
12	42.08574275888022
13	43.57338264921346
14	43.33195638692954
15	43.517287246557075
16	43.56947687366662
17	43.62055373329514
18	43.5555683593247
19	43.30192338118103
20	43.27643418784833
21	43.45261542130519
22	43.3876159107499
23	43.498828562201126
24	43.5157261414142
25	43.51129083134163
26	43.60427733951811
27	43.69126603215478
28	43.625627943472054
29	43.67280167113135
30	43.74381578162154
31	43.808988889689736
32	43.8378274860622
33	43.90369568829169
34	43.876814871659
35	43.92506224946069
36	43.964903968563036
37	43.96505511198715
38	43.90022927298276
39	44.0264027308845
40	44.01459555806907
41	43.99151465900939
42	44.08997251758699
43	44.02498526513469
44	44.07552895833125
45	44.14379970556007
46	44.05623610021321
47	44.063131750630816
48	44.162385436718004
49	44.17752054676554
50	44.1140615056878
51	44.20112078762746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16868.0
1	10979.0
2	5090.0
3	6970.0
4	8850.0
5	11487.5
6	14125.0
7	23041.5
8	31958.0
9	29895.0
10	27832.0
11	34420.0
12	41008.0
13	55994.0
14	70980.0
15	98397.0
16	125814.0
17	177218.0
18	228622.0
19	291965.0
20	355308.0
21	429823.0
22	504338.0
23	601521.0
24	698704.0
25	824515.0
26	1100279.0
27	1250232.0
28	1371697.5
29	1493163.0
30	1611506.5
31	1729850.0
32	1866077.5
33	2002305.0
34	2136241.0
35	2270177.0
36	2444242.5
37	2618308.0
38	2738317.5
39	2858327.0
40	2968628.5
41	3078930.0
42	3117445.5
43	3155961.0
44	3167054.5
45	3178148.0
46	3131857.5
47	3085567.0
48	3005802.5
49	2926038.0
50	2779467.0
51	2632896.0
52	2449931.5
53	2266967.0
54	2070085.5
55	1873204.0
56	1686872.0
57	1500540.0
58	1315076.5
59	1129613.0
60	988456.0
61	847299.0
62	722927.5
63	598556.0
64	509287.5
65	420019.0
66	347987.0
67	275955.0
68	253559.5
69	231164.0
70	203620.5
71	176077.0
72	148549.0
73	121021.0
74	101759.0
75	68795.5
76	55094.0
77	51700.5
78	48307.0
79	48103.5
80	47900.0
81	42379.5
82	36859.0
83	32096.5
84	27334.0
85	24901.5
86	22469.0
87	26166.0
88	29863.0
89	18907.5
90	7952.0
91	4639.5
92	1327.0
93	890.0
94	453.0
95	273.0
96	93.0
97	56.0
98	19.0
99	13.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03202590593090416
2	0.0
3	0.007209869993156602
4	0.030794736484898298
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.003622649664866155
14	0.0
15	0.0
16	0.0
17	0.0168355778740693
18	4.4286670719635145E-6
19	0.0
20	0.0
21	0.0
22	6.643000607945272E-5
23	0.0
24	0.0
25	0.0
26	0.0
27	0.004269235057372828
28	0.0
29	2.4357668895799332E-5
30	0.0
31	2.2143335359817574E-5
32	0.006414924253739151
33	2.081473523822852E-4
34	0.002502196895659386
35	0.0010761660984871342
36	0.002765702586441215
37	3.897227023327893E-4
38	3.875083687968076E-4
39	9.765210893679549E-4
40	0.0
41	0.002263048873773356
42	0.0011625251063904225
43	9.255914180403746E-4
44	1.040736761911426E-4
45	0.0
46	0.0
47	2.0150435177433994E-4
48	0.0038927983562559295
49	9.743067558319733E-5
50	7.285157333379982E-4
51	0.08519648279689812
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.5160315E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	23.67817735516313
#Duplication Level	Relative count
1	100.0
2	11.529598381284854
3	2.799028271309648
4	1.1809376307555877
5	0.7025673371761164
6	0.4758722763806229
7	0.3734535712278379
8	0.29539053376382496
9	0.21920100919894833
10++	7.324810931323262
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	7213105	3.0147314	23.107403	4
TGTTT	6767465	2.173141	17.821524	6
CTTTG	4618525	1.9781952	22.888847	1
GAGGG	2688210	1.942531	7.2091284	11
GTTTG	4524815	1.8911552	22.173155	7
TTTGT	5709330	1.8333569	17.55329	2
TTTGA	5376785	1.7621132	17.660805	8
TGAGG	3122675	1.7336723	8.930676	10
GTGTT	4103300	1.7149825	22.141533	5
TTGTG	4025605	1.6825094	22.226051	3
TTGAG	3536805	1.5086441	13.81594	9
>>END_MODULE