FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_C_16_TGACCA_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_16_TGACCA_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29390367
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33061 0.11248923839569612 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 13213270 4.2656226 5.644533 17
TGTGT 7195160 4.077572 42.57654 4
AAAAA 7511820 3.379186 3.5725029 47
GTTTG 5576400 3.1602032 42.13406 7
TGTTT 6827135 2.9201496 32.316326 6
GTGTT 5151530 2.9194255 41.992237 5
TTTGA 5958340 2.7233965 34.135765 8
TTGTG 4762965 2.6992214 42.05024 3
CTTTG 4388785 2.6951737 45.791046 1
GGGGG 2000025 2.6362102 6.24407 13
GAGGG 2249815 2.3917503 13.432961 11
TTGAG 3683225 2.2305315 26.834738 9
TTTGT 5164090 2.2088203 32.11286 2
TGAGG 2586425 2.0752692 16.473072 10
AGGGG 1628720 1.731472 7.0562005 12
AACTT 2868525 1.518254 14.259825 2
TGAGC 1720720 1.4961188 6.510825 10
ACTTT 2998915 1.4853576 13.023549 3
TGAGA 2206505 1.4279201 5.0663056 10
TGAGT 2146230 1.2997394 7.810207 10
GAGTG 1578185 1.266288 6.2968545 11
TTGAT 2499540 1.1424723 7.9535713 9
TAACT 2122045 1.1231569 14.017698 1
TTGAC 1596670 1.0477957 6.8774643 9
Produced by FastQC (version 0.10.1)