##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_16_TGACCA_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29390367	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19942054483362	33.0	31.0	34.0	31.0	34.0
2	32.36421712597192	34.0	31.0	34.0	31.0	34.0
3	32.45217849780508	34.0	31.0	34.0	31.0	34.0
4	35.900062050943426	37.0	35.0	37.0	35.0	37.0
5	35.92867867216493	37.0	37.0	37.0	35.0	37.0
6	35.90732204875155	37.0	36.0	37.0	35.0	37.0
7	35.957685999633824	37.0	37.0	37.0	35.0	37.0
8	35.94311333369876	37.0	37.0	37.0	35.0	37.0
9	37.67003477704106	39.0	38.0	39.0	35.0	39.0
10	37.66290907493602	39.0	38.0	39.0	35.0	39.0
11	37.653028626692546	39.0	38.0	39.0	35.0	39.0
12	37.590126656125115	39.0	38.0	39.0	35.0	39.0
13	37.57384366789295	39.0	38.0	39.0	35.0	39.0
14	39.05296538148026	41.0	39.0	41.0	36.0	41.0
15	39.0128222965028	41.0	39.0	41.0	36.0	41.0
16	38.972938956495504	41.0	39.0	41.0	36.0	41.0
17	38.95907893902788	41.0	39.0	41.0	36.0	41.0
18	38.92499294071421	41.0	39.0	41.0	36.0	41.0
19	38.92638992905397	41.0	39.0	41.0	36.0	41.0
20	38.90539424022844	41.0	39.0	41.0	36.0	41.0
21	38.85850823162569	41.0	39.0	41.0	35.0	41.0
22	38.81807767150373	41.0	39.0	41.0	35.0	41.0
23	38.78743909526546	40.0	39.0	41.0	35.0	41.0
24	38.74111599218887	41.0	39.0	41.0	35.0	41.0
25	38.668800222875745	40.0	39.0	41.0	35.0	41.0
26	38.59235418870407	40.0	39.0	41.0	34.0	41.0
27	38.558047165590004	40.0	39.0	41.0	34.0	41.0
28	38.51955016417454	40.0	38.0	41.0	34.0	41.0
29	38.43487027569271	40.0	38.0	41.0	34.0	41.0
30	38.393679160250024	40.0	38.0	41.0	34.0	41.0
31	38.33214654992229	40.0	38.0	41.0	34.0	41.0
32	38.279162624951226	40.0	38.0	41.0	34.0	41.0
33	38.20795085682326	40.0	38.0	41.0	34.0	41.0
34	38.151210599037434	40.0	38.0	41.0	34.0	41.0
35	38.09322622613049	40.0	38.0	41.0	33.0	41.0
36	38.03923965971572	40.0	38.0	41.0	33.0	41.0
37	37.97256294894174	40.0	38.0	41.0	33.0	41.0
38	37.91619587465512	40.0	38.0	41.0	33.0	41.0
39	37.825467813994976	40.0	38.0	41.0	33.0	41.0
40	37.74765640047979	40.0	38.0	41.0	33.0	41.0
41	37.9259156239866	40.0	38.0	41.0	33.0	41.0
42	37.967027597852045	40.0	38.0	41.0	33.0	41.0
43	37.94800007090759	40.0	38.0	41.0	33.0	41.0
44	37.9141548317515	40.0	38.0	41.0	33.0	41.0
45	37.85058373037669	40.0	38.0	41.0	33.0	41.0
46	37.80535571399976	40.0	38.0	41.0	33.0	41.0
47	37.7538914706305	40.0	38.0	41.0	33.0	41.0
48	37.692016843478	40.0	38.0	41.0	33.0	41.0
49	37.62399557650981	40.0	37.0	41.0	32.0	41.0
50	37.54395295574227	40.0	37.0	41.0	32.0	41.0
51	36.056953014571064	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	8.0
6	243.0
7	1918.0
8	7129.0
9	11989.0
10	13579.0
11	16984.0
12	23172.0
13	31665.0
14	39158.0
15	44590.0
16	47525.0
17	49249.0
18	51231.0
19	52891.0
20	56079.0
21	60253.0
22	66261.0
23	73673.0
24	84288.0
25	96805.0
26	111300.0
27	130348.0
28	155129.0
29	187716.0
30	228162.0
31	284784.0
32	365574.0
33	484730.0
34	684549.0
35	1018184.0
36	1549289.0
37	2501024.0
38	5716472.0
39	1.5141771E7
40	2645.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.49958801090467	26.55189335843364	31.475616427859755	23.472902202801933
2	20.59677711923494	28.554864718268014	33.22939640237056	17.618961760126485
3	22.16566400821058	27.543813930598414	31.714803016920477	18.575719044270524
4	20.754615735353163	25.23267185235091	33.63382740246218	20.378885009833745
5	27.358372233082566	25.27871054673173	28.864312670066944	18.49860455011876
6	20.282152711462263	25.06543118363987	35.70197121776522	18.950444887132655
7	25.249441116445244	25.431596008615415	30.821685260496608	18.497277614442737
8	21.847441692070706	25.67620116392981	33.7003651444432	18.77599199955629
9	22.257584913989167	25.993710940728782	32.96089586988133	18.787808275400728
10	21.707409560697577	27.884754307109016	31.647864042645036	18.75997208954837
11	26.414348359616874	25.695141567907697	28.786268684837392	19.104241387638037
12	20.738935414504965	30.896657973411624	29.081897446455173	19.28250916562824
13	23.95982518741235	26.147085527312708	29.976250116932263	19.916839168342683
14	24.336986411290486	26.664553875444525	28.789161347000707	20.20929836626428
15	23.342814146527424	28.19123409946089	28.1240204963041	20.341931257707586
16	23.55382059018111	27.062874026881666	28.731503824804182	20.651801558133034
17	23.380794458963685	27.110640697465243	28.90622328467927	20.602341558891798
18	22.974018365699994	27.14659657895745	29.088664250307467	20.79072080503509
19	22.58787728410027	27.54915546152664	29.402854086666814	20.460113167706275
20	22.259748144729503	27.575502243037732	29.460745461713	20.704004150519765
21	22.31892417294449	27.726926336395785	29.314002818025962	20.64014667263376
22	22.01691752537079	27.888295178668958	29.534565740035646	20.560221555924606
23	22.05172088794647	28.01629530528349	29.313162341281373	20.618821465488672
24	21.87502233350842	28.094297806929447	29.277648747291817	20.753031112270317
25	21.952750021946823	28.233900049065607	29.106427458775702	20.706922470211868
26	21.917960265235966	28.192230337558076	29.13577140023048	20.754037996975473
27	21.915996369670747	28.1623486183189	29.00312808289433	20.918526929116023
28	21.85154940561375	28.202829671881368	29.080278380590936	20.865342541913947
29	21.95481900474672	28.149985782718538	28.979453168492764	20.91574204404198
30	21.926026091644864	28.191171769343175	28.859484553990555	21.023317585021402
31	22.026596986957784	28.143228929908904	28.827142994020843	21.00303108911247
32	22.027781037733433	28.210952632086304	28.706423547056858	21.054842783123405
33	22.012278399310947	28.143073133297563	28.71355973129296	21.131088736098533
34	22.021205587987694	28.182129653091724	28.65568014550869	21.1409846134119
35	22.070828895901666	28.152369833522812	28.597528622795444	21.17927264778009
36	22.022143216922224	28.20979561819356	28.61270767560332	21.15535348928089
37	22.063564456289424	28.110847844834712	28.623923288534204	21.201664410341657
38	21.988260493886152	28.138765582757095	28.705732145728174	21.167241777628572
39	22.007427874707677	28.069404287252674	28.674420340279035	21.248747497760615
40	22.007793212941035	28.110913462338548	28.64169621784105	21.239597106879366
41	22.045954701059543	28.08462565388024	28.603502867029736	21.26591677803048
42	21.948712472451593	28.051832012922272	28.636548049847367	21.362907464778772
43	21.997550685779053	28.137233064482782	28.566994631553815	21.298221618184346
44	22.04116329588818	28.06187170640631	28.534381328025983	21.362583669679534
45	22.06848513647848	28.072543780115357	28.53133099472772	21.327640088678443
46	21.926680387748068	28.190519003216714	28.452836664312354	21.429963944722864
47	22.025940204451413	28.169522022622097	28.404546335352943	21.399991437573547
48	21.95240905111471	28.106417870421186	28.439904321914966	21.501268756549138
49	21.97859669641777	28.179654083657564	28.403997946682136	21.437751273242537
50	21.970886189316598	28.143579532188483	28.437122928862824	21.448411349632092
51	22.01064491447143	28.125268842363	28.40415309555527	21.459933147610304
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.97249021370661
2	38.215738879361425
3	40.741383052481105
4	41.13350074518691
5	45.856976783201326
6	39.232597598594914
7	43.74671873088798
8	40.62343369162699
9	41.0453931893899
10	40.46738165024594
11	45.518589747254914
12	40.021444580133206
13	43.87666435575503
14	44.54628477755477
15	43.68474540423501
16	44.20562214831414
17	43.983136017855486
18	43.76473917073508
19	43.04799045180655
20	42.96375229524927
21	42.95907084557825
22	42.5771390812954
23	42.670542353435145
24	42.62805344577873
25	42.65967249215869
26	42.67199826221144
27	42.83452329878677
28	42.7168919475277
29	42.870561048788694
30	42.94934367666627
31	43.02962807607025
32	43.08262382085684
33	43.14336713540948
34	43.16219020139959
35	43.25010154368175
36	43.177496706203115
37	43.26522886663108
38	43.15550227151472
39	43.25617537246829
40	43.2473903198204
41	43.31187147909002
42	43.31161993723037
43	43.295772303963396
44	43.40374696556771
45	43.39612522515692
46	43.356644332470935
47	43.42593164202496
48	43.45367780766385
49	43.41634796966031
50	43.41929753894869
51	43.470578062081735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21756.0
1	13526.5
2	5297.0
3	6938.5
4	8580.0
5	32252.0
6	55924.0
7	67941.0
8	79958.0
9	58106.0
10	36254.0
11	34389.5
12	32525.0
13	39980.0
14	47435.0
15	66486.5
16	85538.0
17	120210.0
18	154882.0
19	196229.5
20	237577.0
21	289093.0
22	340609.0
23	407215.0
24	473821.0
25	555117.0
26	745499.0
27	854585.0
28	936520.5
29	1018456.0
30	1095469.0
31	1172482.0
32	1266136.0
33	1359790.0
34	1439173.0
35	1518556.0
36	1645440.0
37	1772324.0
38	1846253.0
39	1920182.0
40	1990307.5
41	2060433.0
42	2065105.5
43	2069778.0
44	2063484.0
45	2057190.0
46	1999589.0
47	1941988.0
48	1873880.0
49	1805772.0
50	1710203.0
51	1614634.0
52	1490597.5
53	1366561.0
54	1251368.5
55	1136176.0
56	1020327.0
57	904478.0
58	799601.5
59	694725.0
60	607208.0
61	519691.0
62	443915.5
63	368140.0
64	313022.0
65	257904.0
66	217596.5
67	177289.0
68	157482.0
69	137675.0
70	124287.5
71	110900.0
72	95909.5
73	80919.0
74	68829.5
75	47733.5
76	38727.0
77	35943.0
78	33159.0
79	32745.0
80	32331.0
81	28450.5
82	24570.0
83	22051.5
84	19533.0
85	17560.0
86	15587.0
87	17840.0
88	20093.0
89	13849.0
90	7605.0
91	5041.0
92	2477.0
93	1848.5
94	1220.0
95	943.5
96	667.0
97	516.5
98	366.0
99	230.5
100	95.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.84643597679471E-5
2	0.003640648651988592
3	0.0
4	0.006012173988844713
5	0.014103260432236182
6	0.0076181423661705215
7	0.026134413360676984
8	0.006794743325253475
9	0.012854551969357851
10	0.007121380961319742
11	0.026263707424953216
12	0.029206848624925303
13	0.012575548988551248
14	0.014562594607954367
15	0.0016433956064584017
16	0.0412754287824987
17	0.002143559486684872
18	4.933589294750895E-4
19	0.0060257838903474735
20	0.0010649747925910553
21	9.86717858950179E-4
22	0.017352624416020392
23	0.0010445599403369138
24	0.021330118130202323
25	0.0039026392559167434
26	0.004392595710016142
27	0.008162538426280964
28	0.0013167579703921356
29	0.06009792256081729
30	0.006913829963402634
31	9.220708268120639E-4
32	0.005988356661214881
33	0.009605187985573641
34	0.01668914171776079
35	0.013606499027385402
36	0.011258791018159114
37	0.016416943687705566
38	0.027937725309792834
39	0.02105451762477141
40	0.022435922627301663
41	0.026175243065185268
42	0.016403333786202803
43	0.02256521669157789
44	0.016066488724009467
45	0.014273384201020695
46	0.029992820436709755
47	0.021003480494136056
48	0.04886294887028801
49	0.012868161870860612
50	0.04576329380303417
51	0.0965112140314546
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9390367E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.029987422084226
#Duplication Level	Relative count
1	100.0
2	11.062912243431137
3	3.0734198792766776
4	1.5462507712172193
5	0.919736164204072
6	0.6773990421005095
7	0.5260178477156069
8	0.44396669613723533
9	0.3409257150853268
10++	8.620459359237751
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33061	0.11248923839569612	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13213270	4.2656226	5.644533	17
TGTGT	7195160	4.077572	42.57654	4
AAAAA	7511820	3.379186	3.5725029	47
GTTTG	5576400	3.1602032	42.13406	7
TGTTT	6827135	2.9201496	32.316326	6
GTGTT	5151530	2.9194255	41.992237	5
TTTGA	5958340	2.7233965	34.135765	8
TTGTG	4762965	2.6992214	42.05024	3
CTTTG	4388785	2.6951737	45.791046	1
GGGGG	2000025	2.6362102	6.24407	13
GAGGG	2249815	2.3917503	13.432961	11
TTGAG	3683225	2.2305315	26.834738	9
TTTGT	5164090	2.2088203	32.11286	2
TGAGG	2586425	2.0752692	16.473072	10
AGGGG	1628720	1.731472	7.0562005	12
AACTT	2868525	1.518254	14.259825	2
TGAGC	1720720	1.4961188	6.510825	10
ACTTT	2998915	1.4853576	13.023549	3
TGAGA	2206505	1.4279201	5.0663056	10
TGAGT	2146230	1.2997394	7.810207	10
GAGTG	1578185	1.266288	6.2968545	11
TTGAT	2499540	1.1424723	7.9535713	9
TAACT	2122045	1.1231569	14.017698	1
TTGAC	1596670	1.0477957	6.8774643	9
>>END_MODULE