FastQCFastQC Report
Mon 11 Aug 2014
140701_JD_C_16_TGACCA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_16_TGACCA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29390367
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41361 0.14072978401392539 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12083735 4.0345116 5.178818 16
TGTGT 5825220 3.4907618 48.749527 4
AAAAA 7436355 3.1793666 3.3074615 45
GTTTG 4355935 2.6102931 48.07327 7
TGTTT 5609255 2.5090194 36.45368 6
CTTTG 3936040 2.4729514 50.45756 1
GTGTT 3909385 2.342698 47.966534 5
TTTGA 4784560 2.2486348 37.932205 8
GAGGG 1957880 2.2125351 14.703665 11
TTGTG 3609675 2.1630971 48.012054 3
GGGGG 1472145 2.1212196 5.9205713 13
TTTGT 4682100 2.094303 36.270355 2
GAGAG 2290875 2.0303674 5.0233054 11
TGAGG 2268500 1.9135238 18.598217 10
TTGAG 2976910 1.8743544 29.836739 9
AGGGG 1422540 1.6075653 7.718156 12
TGAGC 1619515 1.4322813 6.958151 10
TGAGA 2115010 1.39919 5.4884768 10
TGAGT 1991250 1.2537527 8.7317705 10
GAGTG 1456535 1.2286154 6.935703 11
TTGAT 2250100 1.0574961 8.994572 9
TTGAC 1482175 0.978439 7.5758476 9
Produced by FastQC (version 0.10.1)