##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_16_TGACCA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29390367	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49524883442252	34.0	31.0	34.0	31.0	34.0
2	32.79291221508054	34.0	31.0	34.0	31.0	34.0
3	32.913232046404865	34.0	31.0	34.0	31.0	34.0
4	36.387255695037766	37.0	37.0	37.0	35.0	37.0
5	36.298106995397504	37.0	37.0	37.0	35.0	37.0
6	36.26623352474639	37.0	37.0	37.0	35.0	37.0
7	36.27620386638928	37.0	37.0	37.0	35.0	37.0
8	36.2710109404214	37.0	37.0	37.0	35.0	37.0
9	38.03305154372519	39.0	38.0	39.0	35.0	39.0
10	38.03275825034781	39.0	38.0	39.0	35.0	39.0
11	38.02057963413659	39.0	38.0	39.0	35.0	39.0
12	38.00001388209953	39.0	38.0	39.0	35.0	39.0
13	37.98479515414013	39.0	38.0	39.0	35.0	39.0
14	39.470908308154165	41.0	39.0	41.0	37.0	41.0
15	39.49465813067254	41.0	39.0	41.0	37.0	41.0
16	39.4701274740802	41.0	39.0	41.0	37.0	41.0
17	39.46046022494377	41.0	39.0	41.0	37.0	41.0
18	39.4252171468291	41.0	39.0	41.0	37.0	41.0
19	39.44373345184835	41.0	39.0	41.0	37.0	41.0
20	39.420123709241196	41.0	39.0	41.0	37.0	41.0
21	39.39551608865585	41.0	39.0	41.0	36.0	41.0
22	39.37167150719826	41.0	39.0	41.0	36.0	41.0
23	39.339579223355734	41.0	39.0	41.0	36.0	41.0
24	39.29303757928576	41.0	39.0	41.0	36.0	41.0
25	39.25058547244409	41.0	39.0	41.0	36.0	41.0
26	39.15646374881947	40.0	39.0	41.0	36.0	41.0
27	39.10493244266055	40.0	39.0	41.0	36.0	41.0
28	39.03879158773349	40.0	39.0	41.0	36.0	41.0
29	38.98831923398575	40.0	39.0	41.0	35.0	41.0
30	38.930785893214605	40.0	39.0	41.0	35.0	41.0
31	38.8620119306438	40.0	38.0	41.0	35.0	41.0
32	38.77523312315222	40.0	38.0	41.0	35.0	41.0
33	38.703280670159714	40.0	38.0	41.0	35.0	41.0
34	38.835547647295456	40.0	38.0	41.0	35.0	41.0
35	38.85595936927225	40.0	38.0	41.0	35.0	41.0
36	38.85516931449002	40.0	38.0	41.0	35.0	41.0
37	38.816072184467785	40.0	38.0	41.0	35.0	41.0
38	38.77499991748997	40.0	38.0	41.0	35.0	41.0
39	38.53993361158096	40.0	38.0	41.0	35.0	41.0
40	38.48296947091542	40.0	38.0	41.0	34.0	41.0
41	38.537867424384324	40.0	38.0	41.0	35.0	41.0
42	38.301569048117024	40.0	38.0	41.0	34.0	41.0
43	38.326251693284405	40.0	38.0	41.0	34.0	41.0
44	38.30431818017107	40.0	38.0	41.0	34.0	41.0
45	38.322950475575894	40.0	38.0	41.0	34.0	41.0
46	38.28610595437614	40.0	38.0	41.0	34.0	41.0
47	38.2196080096584	40.0	38.0	41.0	34.0	41.0
48	38.17221064303144	40.0	38.0	41.0	34.0	41.0
49	38.039398895563295	40.0	38.0	41.0	33.0	41.0
50	37.85947902589988	40.0	38.0	41.0	33.0	41.0
51	37.76215329328824	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	7.0
11	13.0
12	50.0
13	88.0
14	169.0
15	262.0
16	416.0
17	790.0
18	1459.0
19	2804.0
20	5148.0
21	9385.0
22	15644.0
23	25008.0
24	37348.0
25	52939.0
26	72998.0
27	98883.0
28	130555.0
29	171467.0
30	221284.0
31	284699.0
32	365726.0
33	482715.0
34	680228.0
35	1015076.0
36	1538745.0
37	2571765.0
38	5873177.0
39	1.5723325E7
40	8187.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.518714038169776	28.06407318542173	29.326174821191493	25.091037955217004
2	19.454585238762075	27.706584950096065	33.61951553718264	19.21931427395922
3	18.827819047162112	26.65478435059502	34.596564404569044	19.92083219767383
4	19.866389008448532	26.485779943382393	34.13747185848144	19.510359189687637
5	26.35320273476	26.445637783291374	28.385936793507888	18.815222688440738
6	21.055861602544805	26.287283176831373	33.12442134526595	19.532433875357867
7	26.158553923467508	26.81712344728461	27.77941153303734	19.244911096210537
8	20.830818478721277	26.766219013188913	32.77016581657521	19.632796691514603
9	20.26489495690884	27.07416685201651	33.03666470037614	19.6242734906985
10	20.560583676957826	26.89787439537587	32.97305542322761	19.56848650443868
11	25.922360887837844	26.721030737724373	27.71006908488077	19.646539289557015
12	20.52723261332531	32.202694168466834	27.48714910569167	19.782924112516188
13	23.686091275768835	27.16586512451181	28.74274477886079	20.40529882085856
14	22.669158911829854	27.516951387507344	29.190397656483842	20.623492044178963
15	22.940754703743576	27.43925586230345	28.851769697193642	20.768219736759328
16	22.623647401204618	27.458677191747892	28.964102421722053	20.95357298532543
17	22.733717654235672	27.54433086512972	28.797894759696323	20.92405672093829
18	22.30955621000284	27.624410244649905	29.080635779787016	20.985397765560243
19	21.927813967072954	28.002147778556154	29.394712900318666	20.675325354052234
20	21.661243631289125	27.991661349448272	29.475824510799747	20.871270508462857
21	21.845671406553038	28.01218848338981	29.230897320880683	20.91124278917647
22	21.713504602701228	28.060669573516478	29.345982609938297	20.879843213843998
23	21.862530672039586	28.128992060561885	29.11282462039348	20.895652647005054
24	21.644932164338062	28.204135729234004	29.129081647738523	21.021850458689407
25	21.7448050240407	28.317853261240323	28.969383063505127	20.96795865121385
26	21.75308664910513	28.261467439314387	28.95328935497811	21.03215655660237
27	21.79258697094002	28.262647958359405	28.784016284725734	21.16074878597484
28	21.720684876102432	28.30692110785823	28.894327859192774	21.07806615684656
29	21.82476393795164	28.220301849932728	28.848483887543285	21.106450324572346
30	21.757418000258383	28.210226160156488	28.847485980695648	21.18486985888948
31	21.87238073112277	28.188938698875326	28.74267132098221	21.196009249019696
32	21.885159743385415	28.302742926764218	28.61434936486573	21.197747964984632
33	21.89647763801855	28.21556780607974	28.611722225290126	21.276232330611585
34	21.84872374109414	28.24024636157602	28.628512325156123	21.282517572173713
35	21.913130653525535	28.220172882893635	28.552016279785402	21.314680183795424
36	21.90066909848383	28.292305988405058	28.512009968170656	21.295014944940455
37	21.963090213510913	28.176466253064707	28.51933841742232	21.34110511600206
38	21.896921598149046	28.19275780233241	28.602526348023577	21.30779425149497
39	21.960421646029584	28.277293118369464	28.458578930866242	21.303706304734714
40	21.949372051053327	28.257986707005056	28.48794981022183	21.30469143171979
41	21.937753877053193	28.119262661860176	28.562921222993705	21.38006223809293
42	21.96167170267065	28.189837133572652	28.431347563893684	21.417143599863014
43	21.905770245566696	28.289038543964175	28.4376361959423	21.36755501452683
44	21.97096691919412	28.174546898681253	28.430777382061912	21.423708800062716
45	22.004621446203785	28.059588367848555	28.496173593204876	21.439616592742787
46	21.842942621301734	28.20562261097318	28.46544924056239	21.485985527162693
47	21.950297273260134	28.26250253527533	28.370157105240747	21.417043086223792
48	21.86917729073634	28.189575504504372	28.407713163048786	21.533534041710503
49	21.96232619673748	28.23293692081103	28.330050452286315	21.474686430165175
50	22.004012704527263	28.40960371999424	28.15613106879709	21.430252506681406
51	21.94694717874874	28.156356875510564	28.343162344321964	21.553533601418735
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.60975199338678
2	38.67389951272129
3	38.74865124483594
4	39.37674819813617
5	45.16842542320074
6	40.588295477902676
7	45.403465019678045
8	40.463615170235876
9	39.88916844760734
10	40.12907018139651
11	45.56890017739486
12	40.310156725841495
13	44.0913900966274
14	43.29265095600881
15	43.7089744405029
16	43.577220386530044
17	43.65777437517396
18	43.294953975563075
19	42.603139321125184
20	42.53251413975198
21	42.75691419572951
22	42.593347816545226
23	42.75818331904464
24	42.66678262302747
25	42.71276367525455
26	42.7852432057075
27	42.95333575691486
28	42.79875103294899
29	42.93121426252399
30	42.94228785914787
31	43.06838998014247
32	43.08290770837005
33	43.17270996863014
34	43.13124131326786
35	43.22781083732096
36	43.195684043424286
37	43.30419532951297
38	43.20471584964402
39	43.2641279507643
40	43.25406348277311
41	43.317816115146115
42	43.37881530253366
43	43.27332526009353
44	43.39467571925683
45	43.44423803894657
46	43.32892814846443
47	43.367340359483926
48	43.40271133244684
49	43.43701262690266
50	43.43426521120867
51	43.50048078016747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35106.0
1	21318.5
2	7531.0
3	9057.5
4	10584.0
5	20069.5
6	29555.0
7	51923.5
8	74292.0
9	54424.5
10	34557.0
11	33205.0
12	31853.0
13	40148.0
14	48443.0
15	69299.0
16	90155.0
17	127069.5
18	163984.0
19	208201.5
20	252419.0
21	302363.5
22	352308.0
23	419646.0
24	486984.0
25	575129.0
26	776279.5
27	889285.0
28	963801.0
29	1038317.0
30	1108354.0
31	1178391.0
32	1270002.5
33	1361614.0
34	1438587.0
35	1515560.0
36	1640804.5
37	1766049.0
38	1833960.0
39	1901871.0
40	1968290.0
41	2034709.0
42	2037825.5
43	2040942.0
44	2038564.0
45	2036186.0
46	1979223.5
47	1922261.0
48	1859135.5
49	1796010.0
50	1702058.0
51	1608106.0
52	1494008.0
53	1379910.0
54	1261730.5
55	1143551.0
56	1026474.0
57	909397.0
58	802794.0
59	696191.0
60	608123.5
61	520056.0
62	443608.5
63	367161.0
64	311317.5
65	255474.0
66	215977.0
67	176480.0
68	157013.0
69	137546.0
70	124533.0
71	111520.0
72	96073.5
73	80627.0
74	68610.0
75	46731.5
76	36870.0
77	34635.0
78	32400.0
79	31973.0
80	31546.0
81	27659.5
82	23773.0
83	21054.0
84	18335.0
85	16782.5
86	15230.0
87	17657.0
88	20084.0
89	12934.0
90	5784.0
91	3400.5
92	1017.0
93	696.5
94	376.0
95	225.5
96	75.0
97	48.0
98	21.0
99	12.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031353810586985865
2	0.0
3	0.0073391393853639185
4	0.03198667100686425
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.004038738270944354
14	0.0
15	0.0
16	0.0
17	0.01710764618897069
18	6.804950751380546E-6
19	0.0
20	0.0
21	0.0
22	5.7842081386734645E-5
23	0.0
24	0.0
25	0.0
26	0.0
27	0.00461035413406032
28	0.0
29	3.402475375690273E-6
30	0.0
31	3.0622278381212456E-5
32	0.00685258540664021
33	2.0414852254141638E-4
34	0.0024463797951213063
35	0.0010173401373313915
36	0.0027968347588174045
37	3.8447971745300083E-4
38	3.9808961895576193E-4
39	0.001061572317215365
40	0.0
41	0.002208206518822987
42	0.0011296218247291705
43	9.050584499336126E-4
44	1.0547673664639845E-4
45	0.0
46	0.0
47	2.3136832554693858E-4
48	0.0038345897484029375
49	8.165940901656656E-5
50	7.281297303977184E-4
51	0.08708635724079254
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9390367E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.398000147318804
#Duplication Level	Relative count
1	100.0
2	11.510888504542503
3	3.380676521079399
4	1.6318290244388578
5	0.9862985514141875
6	0.7451890619876423
7	0.5150098694151005
8	0.43849779143707684
9	0.38963293491329704
10++	8.993705434929371
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41361	0.14072978401392539	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	12083735	4.0345116	5.178818	16
TGTGT	5825220	3.4907618	48.749527	4
AAAAA	7436355	3.1793666	3.3074615	45
GTTTG	4355935	2.6102931	48.07327	7
TGTTT	5609255	2.5090194	36.45368	6
CTTTG	3936040	2.4729514	50.45756	1
GTGTT	3909385	2.342698	47.966534	5
TTTGA	4784560	2.2486348	37.932205	8
GAGGG	1957880	2.2125351	14.703665	11
TTGTG	3609675	2.1630971	48.012054	3
GGGGG	1472145	2.1212196	5.9205713	13
TTTGT	4682100	2.094303	36.270355	2
GAGAG	2290875	2.0303674	5.0233054	11
TGAGG	2268500	1.9135238	18.598217	10
TTGAG	2976910	1.8743544	29.836739	9
AGGGG	1422540	1.6075653	7.718156	12
TGAGC	1619515	1.4322813	6.958151	10
TGAGA	2115010	1.39919	5.4884768	10
TGAGT	1991250	1.2537527	8.7317705	10
GAGTG	1456535	1.2286154	6.935703	11
TTGAT	2250100	1.0574961	8.994572	9
TTGAC	1482175	0.978439	7.5758476	9
>>END_MODULE