FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_7_ATCACG_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_7_ATCACG_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 44627725
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 88936 0.19928418936882844 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61607 0.13804647223222785 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 24359685 6.067849 7.9015613 18
TGTGT 10180915 3.9033172 34.376568 4
AAAAA 11094120 3.8144042 4.1046386 47
TGTTT 9254310 2.8598886 27.738214 6
GTTTG 7443450 2.8537858 33.67887 7
GTGTT 7126880 2.7324138 33.802315 5
TTTGA 8090300 2.6666367 29.18612 8
CTTTG 6273320 2.5769877 36.54421 1
TTGTG 6646975 2.5484207 33.858788 3
GAGGG 3602060 2.2671287 10.003064 11
TTTGT 7139565 2.2063622 27.62227 2
TTGAG 5041545 2.0616024 20.899662 9
TGAGG 3868775 1.9627125 12.481916 10
AGGGG 2677370 1.6851307 5.657891 12
ACTTT 4535950 1.6018999 14.751484 3
AACTT 4160375 1.5670892 16.086576 2
TGAGC 2686505 1.4602888 5.030178 10
TGAGT 2975895 1.216911 5.971844 10
TAACT 3104095 1.16922 15.859631 1
TTGAT 3539825 1.1667588 7.2709956 9
TTGAC 2232940 0.97833174 5.888865 9
Produced by FastQC (version 0.10.1)