##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_7_ATCACG_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	44627725	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27122756089404	34.0	31.0	34.0	31.0	34.0
2	32.42523482879757	34.0	31.0	34.0	31.0	34.0
3	32.48768737371219	34.0	31.0	34.0	31.0	34.0
4	35.91141072057785	37.0	37.0	37.0	35.0	37.0
5	35.934966369000435	37.0	37.0	37.0	35.0	37.0
6	35.90769858871363	37.0	36.0	37.0	35.0	37.0
7	35.94991465507148	37.0	37.0	37.0	35.0	37.0
8	35.902498592522925	37.0	37.0	37.0	35.0	37.0
9	37.658334813168274	39.0	38.0	39.0	35.0	39.0
10	37.6017492937406	39.0	38.0	39.0	35.0	39.0
11	37.61163474051165	39.0	38.0	39.0	35.0	39.0
12	37.58538522857708	39.0	38.0	39.0	35.0	39.0
13	37.583659081882395	39.0	38.0	39.0	35.0	39.0
14	39.035955608312996	41.0	39.0	41.0	36.0	41.0
15	39.00568592730192	41.0	39.0	41.0	36.0	41.0
16	38.98913424334312	41.0	39.0	41.0	36.0	41.0
17	38.95688084032964	41.0	39.0	41.0	36.0	41.0
18	38.890005282590586	41.0	39.0	41.0	36.0	41.0
19	38.8980290615307	41.0	39.0	41.0	35.0	41.0
20	38.87944642035865	41.0	39.0	41.0	35.0	41.0
21	38.847266850371604	41.0	39.0	41.0	35.0	41.0
22	38.79466228672871	40.0	39.0	41.0	35.0	41.0
23	38.73574969371618	40.0	39.0	41.0	35.0	41.0
24	38.687970897015255	40.0	39.0	41.0	35.0	41.0
25	38.62232307382911	40.0	39.0	41.0	35.0	41.0
26	38.566707848092186	40.0	39.0	41.0	34.0	41.0
27	38.52804827044175	40.0	38.0	41.0	34.0	41.0
28	38.479267361264775	40.0	38.0	41.0	34.0	41.0
29	38.36154567592231	40.0	38.0	41.0	34.0	41.0
30	38.30516487228511	40.0	38.0	41.0	34.0	41.0
31	38.26910477735533	40.0	38.0	41.0	34.0	41.0
32	38.215019385370866	40.0	38.0	41.0	34.0	41.0
33	38.14628511760346	40.0	38.0	41.0	34.0	41.0
34	38.09063780419011	40.0	38.0	41.0	33.0	41.0
35	38.02928108031498	40.0	38.0	41.0	33.0	41.0
36	37.971125572724134	40.0	38.0	41.0	33.0	41.0
37	37.90750870675124	40.0	38.0	41.0	33.0	41.0
38	37.82223835071136	40.0	38.0	41.0	33.0	41.0
39	37.7566584449465	40.0	38.0	41.0	33.0	41.0
40	37.67241142137539	40.0	38.0	41.0	33.0	41.0
41	37.85952895873585	40.0	38.0	41.0	33.0	41.0
42	37.84606055988738	40.0	38.0	41.0	33.0	41.0
43	37.84092406682169	40.0	38.0	41.0	33.0	41.0
44	37.77590632280718	40.0	38.0	41.0	33.0	41.0
45	37.74653827861492	40.0	37.0	41.0	33.0	41.0
46	37.68535644602094	40.0	37.0	41.0	33.0	41.0
47	37.633168753280614	40.0	37.0	41.0	33.0	41.0
48	37.54782050843954	40.0	37.0	41.0	32.0	41.0
49	37.461956216679205	40.0	37.0	41.0	32.0	41.0
50	37.383756353253496	40.0	37.0	41.0	32.0	41.0
51	35.86817571363989	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	36.0
5	88.0
6	791.0
7	3487.0
8	11322.0
9	17922.0
10	21039.0
11	26104.0
12	36338.0
13	48549.0
14	59337.0
15	66546.0
16	71190.0
17	74327.0
18	77161.0
19	80315.0
20	85424.0
21	90403.0
22	99335.0
23	110620.0
24	125013.0
25	144467.0
26	168114.0
27	198945.0
28	237861.0
29	287166.0
30	351871.0
31	440318.0
32	570089.0
33	764846.0
34	1093232.0
35	1646897.0
36	2556451.0
37	4059134.0
38	8827128.0
39	2.2172026E7
40	3830.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.8977937262884	26.19260521574141	30.456039452664996	23.453561605305197
2	21.9321272800494	28.363353802609208	30.948102941715582	18.756415975625814
3	22.93275939371365	27.182831394974034	29.965238398733202	19.919170812579125
4	21.745229169408873	25.02650330724973	31.43107372107509	21.797193802266303
5	27.043259133782083	24.981985789898996	28.250236419351587	19.724518656967334
6	21.209588315216994	24.611610212308673	33.81791587521108	20.360885597263252
7	25.077170519116848	24.925092066152303	30.28306208047618	19.714675334254668
8	22.87164164909214	25.160445333466182	32.01380253081656	19.954110486625108
9	22.95031635028799	25.408770254016773	31.677886984775373	19.963026410919866
10	22.881386088073803	27.15757696760854	30.056994784982777	19.904042159334875
11	26.050612390154786	25.162196214054628	28.52396851051547	20.263222885275116
12	21.756018851309435	29.19716218669147	28.680837534455208	20.365981427543893
13	24.067874894195494	25.57219593123542	29.5069493140103	20.85297986055879
14	24.886262855687185	25.989787046114436	28.078759394043985	21.045190704154397
15	23.73699170900196	27.564013861766206	27.516726366140166	21.182268063091676
16	24.261361278871227	26.361954823270317	27.964753567342232	21.411930330516228
17	23.917357698097234	26.454233934989897	28.194790523522556	21.433617843390312
18	23.76235888801323	26.43435703083282	28.295145135914996	21.508138945238947
19	23.411636578795726	26.64332399239345	28.55829549136831	21.386743937442517
20	23.070977534099697	26.700363063369338	28.63768402773273	21.59097537479824
21	23.15585333183139	26.802750993392	28.475827769523242	21.56556790525337
22	22.861612300188515	26.985414425302572	28.687869813168486	21.465103461340423
23	22.83703461267171	27.09682856515565	28.526786656616586	21.53935016555605
24	22.69052136043689	27.133529456545713	28.48745456863227	21.68849461438513
25	22.71180299007232	27.330357501650592	28.33296880078788	21.6248707074892
26	22.70322573616803	27.273484510655386	28.327790790500845	21.69549896267574
27	22.734605415838228	27.22582656136629	28.248173688272683	21.791394334522806
28	22.705330054758775	27.33282607896158	28.20446362780644	21.757380238473203
29	22.77528793960623	27.309313719497457	28.093495538760067	21.821902802136243
30	22.782666257167477	27.290792274437152	28.042867023119882	21.883674445275485
31	22.835799767637017	27.22782236329893	27.970565412860722	21.965812456203334
32	22.887111313785496	27.29935728987606	27.88664552333686	21.926885873001584
33	22.83739491151955	27.228074360685646	27.8583396547904	22.076191073004413
34	22.91528809650619	27.26269988146538	27.800752452734095	22.021259569294333
35	22.962105453315584	27.21677371549807	27.748639467141345	22.072481364044997
36	22.918009833533482	27.195899233254405	27.77732618011602	22.108764753096093
37	22.922855631432533	27.169977999556842	27.789674257258106	22.117492111752515
38	22.921547865233848	27.21613702831151	27.822002135685548	22.040312970769094
39	22.873654444632145	27.110974760783186	27.814969257514182	22.20040153707049
40	22.918017347773155	27.166609080204633	27.790383574264908	22.1249899977573
41	22.92246985583782	27.143806736712072	27.797895052014116	22.135828355435986
42	22.84488010420833	27.134362416576717	27.774181846904167	22.246575632310787
43	22.873465646189803	27.208693143449064	27.704169809341895	22.21367140101924
44	22.93734853700368	27.160105668931607	27.702817189041752	22.199728605022962
45	22.895055173994116	27.148502446096874	27.664810143356515	22.2916322365525
46	22.807239237255224	27.294911038605218	27.584777689992386	22.313072034147172
47	22.882706830294712	27.179781316147338	27.639448282170054	22.2980635713879
48	22.81904979458237	27.09455533863416	27.574076894211714	22.51231797257176
49	22.899360184443804	27.197431172601345	27.566096012606668	22.337112630348184
50	22.835182793750725	27.166185045303504	27.6234940035954	22.375138157350374
51	22.87675577916703	27.125707598506054	27.572575077267725	22.424961545059194
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.351355331593595
2	40.688543255675214
3	42.85193020629277
4	43.54242297167518
5	46.76777779074942
6	41.570473912480246
7	44.79184585337152
8	42.82575213571725
9	42.91334276120786
10	42.78542824740868
11	46.3138352754299
12	42.12200027885332
13	44.92085475475429
14	45.931453559841586
15	44.91925977209363
16	45.673291609387455
17	45.35097554148754
18	45.27049783325218
19	44.79838051623825
20	44.66195290889794
21	44.72142123708476
22	44.32671576152894
23	44.37638477822776
24	44.379015974822025
25	44.33667369756152
26	44.398724698843765
27	44.52599975036103
28	44.46271029323198
29	44.59719074174247
30	44.666340702442966
31	44.80161222384035
32	44.81399718678708
33	44.91358598452396
34	44.93654766580052
35	45.034586817360584
36	45.02677458662958
37	45.040347743185045
38	44.96186083600294
39	45.074055981702635
40	45.043007345530455
41	45.058298211273815
42	45.091455736519116
43	45.08713704720904
44	45.13707714202664
45	45.18668741054661
46	45.1203112714024
47	45.180770401682615
48	45.33136776715413
49	45.23647281479199
50	45.2103209511011
51	45.301717324226225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52553.0
1	31498.0
2	10443.0
3	13877.0
4	17311.0
5	75947.5
6	134584.0
7	164479.0
8	194374.0
9	122778.5
10	51183.0
11	52413.0
12	53643.0
13	61407.0
14	69171.0
15	91614.0
16	114057.0
17	158529.0
18	203001.0
19	252774.0
20	302547.0
21	358733.5
22	414920.0
23	499039.0
24	583158.0
25	688064.0
26	924436.5
27	1055903.0
28	1164963.5
29	1274024.0
30	1377576.0
31	1481128.0
32	1612110.5
33	1743093.0
34	1861388.0
35	1979683.0
36	2160381.0
37	2341079.0
38	2459630.0
39	2578181.0
40	2702175.5
41	2826170.0
42	2883580.5
43	2940991.0
44	2980077.0
45	3019163.0
46	3013250.5
47	3007338.0
48	2965971.5
49	2924605.0
50	2822200.0
51	2719795.0
52	2557132.0
53	2394469.0
54	2229109.5
55	2063750.0
56	1895786.0
57	1727822.0
58	1549961.0
59	1372100.0
60	1227792.5
61	1083485.0
62	937876.5
63	792268.0
64	684154.5
65	576041.0
66	485890.0
67	395739.0
68	360628.0
69	325517.0
70	291674.5
71	257832.0
72	218019.0
73	178206.0
74	149378.5
75	104388.5
76	88226.0
77	80802.0
78	73378.0
79	74941.0
80	76504.0
81	67282.0
82	58060.0
83	52843.5
84	47627.0
85	42115.5
86	36604.0
87	41311.0
88	46018.0
89	31662.0
90	17306.0
91	11548.5
92	5791.0
93	4334.5
94	2878.0
95	2191.5
96	1505.0
97	1141.5
98	778.0
99	490.0
100	202.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004241757786219217
2	0.00188447876292148
3	7.618582394688503E-5
4	0.0027314858644486135
5	0.0010217863446993995
6	0.0036210673970048885
7	0.023801347704818024
8	0.010966277129295746
9	0.013767226539107696
10	0.0018710342057543824
11	0.013097239440280678
12	0.02080769297561101
13	0.0119544520810774
14	0.005023782861438713
15	0.006318941868535759
16	0.03713386689552291
17	0.010789257126595632
18	0.002095110158539338
19	0.029161244495434172
20	0.00488933728976774
21	0.002424501809133224
22	0.024890356835352907
23	0.001216732423622311
24	0.07083040867532459
25	0.020027908659919366
26	0.03547346408538638
27	0.061208587262738576
28	0.01594076328112177
29	0.10672737631147453
30	0.0529894813145864
31	0.021518013745939323
32	0.034483048374076876
33	0.022064759070734616
34	0.036490768911030085
35	0.0246819661992629
36	0.01873723117187802
37	0.025434861400620352
38	0.07426997455057367
39	0.038426785143092106
40	0.0565298813685887
41	0.016010226826485105
42	0.027966919667090355
43	0.03299742480711262
44	0.00941567153602385
45	0.03228038175820076
46	0.04243326317888711
47	0.046753447548581066
48	0.06241187512919379
49	0.016854993168484392
50	0.07133457956909074
51	0.12274656617607103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.4627725E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	34.79396994253993
#Duplication Level	Relative count
1	100.0
2	15.139263572236972
3	5.453095971926627
4	2.8899059712860082
5	1.8036999338026465
6	1.3232352710888398
7	0.9872659069393334
8	0.7779968538462086
9	0.6391959512844422
10++	13.346240630939077
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88936	0.19928418936882844	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61607	0.13804647223222785	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	24359685	6.067849	7.9015613	18
TGTGT	10180915	3.9033172	34.376568	4
AAAAA	11094120	3.8144042	4.1046386	47
TGTTT	9254310	2.8598886	27.738214	6
GTTTG	7443450	2.8537858	33.67887	7
GTGTT	7126880	2.7324138	33.802315	5
TTTGA	8090300	2.6666367	29.18612	8
CTTTG	6273320	2.5769877	36.54421	1
TTGTG	6646975	2.5484207	33.858788	3
GAGGG	3602060	2.2671287	10.003064	11
TTTGT	7139565	2.2063622	27.62227	2
TTGAG	5041545	2.0616024	20.899662	9
TGAGG	3868775	1.9627125	12.481916	10
AGGGG	2677370	1.6851307	5.657891	12
ACTTT	4535950	1.6018999	14.751484	3
AACTT	4160375	1.5670892	16.086576	2
TGAGC	2686505	1.4602888	5.030178	10
TGAGT	2975895	1.216911	5.971844	10
TAACT	3104095	1.16922	15.859631	1
TTGAT	3539825	1.1667588	7.2709956	9
TTGAC	2232940	0.97833174	5.888865	9
>>END_MODULE