FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_7_ATCACG_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_7_ATCACG_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 44627725
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112625 0.2523655418240567 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 21703305 5.6395273 7.1414924 18
AAAAA 11542230 3.7668424 4.001372 47
TGTGT 8004095 3.2440538 37.948574 4
TGTTT 7495235 2.432381 30.486603 6
CTTTG 5505340 2.3209326 38.922127 1
GTTTG 5624640 2.2796624 37.24086 7
TTTGA 6373005 2.1646354 31.45834 8
GTGTT 5303340 2.1494398 37.242638 5
GAGGG 3153915 2.086791 10.2374935 11
TTTGT 6343610 2.0586514 30.240202 2
TTGTG 4881020 1.9782737 37.24923 3
TGAGG 3391410 1.7967176 13.2688675 10
TTGAG 4004000 1.6984941 22.0814 9
AGGGG 2359125 1.5609174 5.7156796 12
TGAGC 2554150 1.4075003 5.3198676 10
TGAGT 2737390 1.1611991 6.2966123 10
TTGAT 3164995 1.0750124 8.239599 9
TGATT 2961020 1.0057311 5.1362824 10
TTGAC 2013660 0.88850254 6.204885 9
Produced by FastQC (version 0.10.1)