##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_7_ATCACG_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	44627725	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73379962792188	34.0	31.0	34.0	31.0	34.0
2	32.95604956336	34.0	31.0	34.0	31.0	34.0
3	33.031438169882065	34.0	31.0	34.0	31.0	34.0
4	36.42465767636598	37.0	37.0	37.0	35.0	37.0
5	36.353513718209925	37.0	37.0	37.0	35.0	37.0
6	36.31860458941163	37.0	37.0	37.0	35.0	37.0
7	36.313652107518365	37.0	37.0	37.0	35.0	37.0
8	36.29721615878918	37.0	37.0	37.0	35.0	37.0
9	38.06086830552084	39.0	38.0	39.0	37.0	39.0
10	38.04907901982456	39.0	38.0	39.0	37.0	39.0
11	38.00882502973208	39.0	38.0	39.0	35.0	39.0
12	37.98521954681759	39.0	38.0	39.0	35.0	39.0
13	37.99294785920635	39.0	38.0	39.0	35.0	39.0
14	39.52487380882624	41.0	40.0	41.0	37.0	41.0
15	39.527214102892316	41.0	39.0	41.0	37.0	41.0
16	39.52130867078705	41.0	39.0	41.0	37.0	41.0
17	39.489980051638305	41.0	39.0	41.0	37.0	41.0
18	39.43823468482877	41.0	39.0	41.0	37.0	41.0
19	39.46637259685543	41.0	39.0	41.0	37.0	41.0
20	39.43332264864498	41.0	39.0	41.0	37.0	41.0
21	39.400594473502736	41.0	39.0	41.0	36.0	41.0
22	39.34339384317708	41.0	39.0	41.0	36.0	41.0
23	39.303959590142675	41.0	39.0	41.0	36.0	41.0
24	39.249388558345736	41.0	39.0	41.0	36.0	41.0
25	39.21296431758509	40.0	39.0	41.0	36.0	41.0
26	39.144743564678684	40.0	39.0	41.0	36.0	41.0
27	39.083492134093774	40.0	39.0	41.0	36.0	41.0
28	39.006112500693234	40.0	39.0	41.0	35.0	41.0
29	38.92959385673368	40.0	39.0	41.0	35.0	41.0
30	38.878336168827786	40.0	38.0	41.0	35.0	41.0
31	38.797870897519424	40.0	38.0	41.0	35.0	41.0
32	38.73913514524883	40.0	38.0	41.0	35.0	41.0
33	38.658865111318136	40.0	38.0	41.0	35.0	41.0
34	38.76633458237004	40.0	38.0	41.0	35.0	41.0
35	38.7712666733516	40.0	38.0	41.0	35.0	41.0
36	38.76845920333156	40.0	38.0	41.0	35.0	41.0
37	38.710484480219414	40.0	38.0	41.0	35.0	41.0
38	38.662846940999124	40.0	38.0	41.0	35.0	41.0
39	38.558001264908754	40.0	38.0	41.0	35.0	41.0
40	38.537063047690644	40.0	38.0	41.0	35.0	41.0
41	38.50199601256842	40.0	38.0	41.0	35.0	41.0
42	38.430807709781305	40.0	38.0	41.0	34.0	41.0
43	38.35416241809324	40.0	38.0	41.0	34.0	41.0
44	38.23074140570688	40.0	38.0	41.0	34.0	41.0
45	38.20069687621316	40.0	38.0	41.0	34.0	41.0
46	38.11139443025608	40.0	38.0	41.0	34.0	41.0
47	38.0896573598587	40.0	38.0	41.0	34.0	41.0
48	38.01402849910006	40.0	37.0	41.0	33.0	41.0
49	37.96587356850478	40.0	37.0	41.0	33.0	41.0
50	38.03466522212369	40.0	37.0	41.0	34.0	41.0
51	37.757860724471165	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	11.0
10	42.0
11	67.0
12	75.0
13	146.0
14	255.0
15	433.0
16	763.0
17	1187.0
18	2177.0
19	4209.0
20	7902.0
21	14150.0
22	23549.0
23	37132.0
24	55751.0
25	78845.0
26	108264.0
27	146697.0
28	193481.0
29	252949.0
30	327959.0
31	422720.0
32	550773.0
33	734372.0
34	1054558.0
35	1614131.0
36	2478337.0
37	4011255.0
38	8670011.0
39	2.3822256E7
40	13261.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.776398900420492	27.499282325519932	28.27264269708614	24.451676076973435
2	21.527433896775207	27.18721668914126	31.143419685965835	20.141929728117706
3	20.387695683214666	26.15736956818254	32.389447889443666	21.06548685915913
4	21.420622666112685	26.102214332237494	31.678274479078443	20.79888852257137
5	25.963803056964572	26.108202989991298	27.818650122688744	20.10934383035539
6	21.965864869887298	25.768518391332062	31.27473975229823	20.99087698648241
7	25.82430540656061	26.24296667598449	27.482899923758158	20.449827993696744
8	21.954213440187687	26.24872094645201	30.99012105143159	20.806944561928713
9	21.26097397974017	26.572434512402328	31.370046310897543	20.79654519695996
10	21.79190684092166	26.256936334066218	31.165483385513625	20.785673439498495
11	25.61107428492604	26.162860553087718	27.400298052334254	20.825767109651984
12	21.694829839522402	30.286518078167774	27.094578538341356	20.924073543968465
13	23.788898044881293	26.597566871266686	28.23408766635539	21.379447417496635
14	23.1556347539562	26.801670934379917	28.51056826221816	21.532126049445722
15	23.347132303965754	26.801883806535066	28.221147280082054	21.629836609417126
16	23.268176901242445	26.787982134424283	28.16345892603757	21.780382038295702
17	23.226339777033466	26.92068960641036	28.04613810401691	21.806832512539266
18	23.107330700814348	26.888002917468906	28.242320216860705	21.76234616485604
19	22.768319469567405	27.074606648669636	28.528653432367435	21.628420449395527
20	22.50272672425045	27.12312805548569	28.59219913181772	21.781946088446137
21	22.63744105904421	27.117150885978646	28.370620927194295	21.874787127782845
22	22.54093839647887	27.21741697565807	28.45673849608063	21.784906131782428
23	22.62536125245013	27.232627251333113	28.30922257408371	21.832788922133044
24	22.483476359146696	27.287962807873356	28.278853560202766	21.94970727277718
25	22.538336874667035	27.424635694515015	28.16689401039376	21.87013342042419
26	22.532629660149606	27.354394605595513	28.12600238977004	21.98697334448485
27	22.55968683144839	27.341832907682388	28.04689013387978	22.051590126989446
28	22.56296954415669	27.42904326850629	28.0301964753973	21.97779071193972
29	22.64331421778726	27.43607029038563	27.918850893698927	22.001764598128183
30	22.591877582825475	27.342000964646978	27.98132775085443	22.08479370167312
31	22.715780380917916	27.275748427687045	27.85644574084832	22.152025450546716
32	22.73468892867333	27.35828579020265	27.795198065561177	22.111827215562847
33	22.682725444342054	27.296114983307625	27.761934801000532	22.259224771349793
34	22.6767939585095	27.397507251922278	27.734654162076687	22.19104462749153
35	22.72364194790886	27.370765547165398	27.695627358716862	22.20996514620888
36	22.78357098456421	27.319066050818314	27.657183764514798	22.24017920010268
37	22.798425575630088	27.3143102040681	27.655493296287	22.231770924014814
38	22.754072720489336	27.321278420533424	27.742767528481455	22.181881330495784
39	22.78406939905814	27.242590055345676	27.67314332501376	22.300197220582422
40	22.746419843336607	27.304705405096787	27.724805891665028	22.224068859901582
41	22.77928514349	27.216254914676608	27.742176186259364	22.262283755574032
42	22.70223645101256	27.284330296852794	27.667705249323156	22.34572800281149
43	22.75299310255568	27.35594487090739	27.595302996756114	22.295759029780815
44	22.85054151436875	27.240483755868578	27.582125700044212	22.32684902971846
45	22.811997520989642	27.189884727093972	27.570497697315954	22.427620054600432
46	22.7043378417589	27.380600018405758	27.548093790327727	22.366968349507616
47	22.735596908563334	27.327828893288164	27.569993210004874	22.366580988143628
48	22.708087783508297	27.24164576722391	27.527060779322564	22.523205669945227
49	22.810250802612053	27.242428333507924	27.55107279163345	22.396248072246568
50	22.72652614915379	27.246080270820283	27.537966950615516	22.48942662941041
51	22.768754820187258	27.220337050756417	27.509832626789414	22.501075502266907
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.22807497739393
2	41.66936362489292
3	41.453182542373796
4	42.21951118868405
5	46.073146887319965
6	42.9567418563697
7	46.27413340025735
8	42.761158002116396
9	42.05751917670014
10	42.57758028042016
11	46.436841394578025
12	42.61890338349087
13	45.16834546237793
14	44.68776080340192
15	44.97696891338288
16	45.04855893953815
17	45.03317228957273
18	44.869676865670385
19	44.39673991896293
20	44.28467281269659
21	44.51222818682706
22	44.3258445282613
23	44.458150174583174
24	44.43318363192388
25	44.40847029509122
26	44.51960300463445
27	44.611276958437834
28	44.540760256096405
29	44.64507881591545
30	44.676671284498596
31	44.867805831464636
32	44.846516144236176
33	44.94195021569185
34	44.86783858600103
35	44.93360709411774
36	45.023750184666895
37	45.0301964996449
38	44.935954050985124
39	45.08426661964056
40	44.97048870323819
41	45.04156889906403
42	45.04796445382405
43	45.048752132336496
44	45.17739054408721
45	45.239617575590074
46	45.071306191266515
47	45.10217789670696
48	45.231293453453524
49	45.20649887485862
50	45.2159527785642
51	45.26983032245416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80588.0
1	47264.5
2	13941.0
3	17658.0
4	21375.0
5	47041.5
6	72708.0
7	126613.5
8	180519.0
9	111202.0
10	41885.0
11	46786.5
12	51688.0
13	60573.5
14	69459.0
15	94837.0
16	120215.0
17	170303.5
18	220392.0
19	274357.0
20	328322.0
21	386068.5
22	443815.0
23	530864.5
24	617914.0
25	729069.5
26	974649.5
27	1109074.0
28	1207998.0
29	1306922.0
30	1402172.0
31	1497422.0
32	1622726.5
33	1748031.0
34	1859131.0
35	1970231.0
36	2144374.5
37	2318518.0
38	2429434.5
39	2540351.0
40	2656814.0
41	2773277.0
42	2827253.5
43	2881230.0
44	2921387.5
45	2961545.0
46	2957797.0
47	2954049.0
48	2924873.0
49	2895697.0
50	2799412.5
51	2703128.0
52	2555780.0
53	2408432.0
54	2245006.0
55	2081580.0
56	1913422.0
57	1745264.0
58	1567285.5
59	1389307.0
60	1244697.0
61	1100087.0
62	952074.0
63	804061.0
64	695097.0
65	586133.0
66	493268.5
67	400404.0
68	367933.0
69	335462.0
70	299999.5
71	264537.0
72	223835.0
73	183133.0
74	153403.5
75	105046.0
76	86418.0
77	79911.5
78	73405.0
79	76626.0
80	79847.0
81	69293.5
82	58740.0
83	52504.0
84	46268.0
85	40965.5
86	35663.0
87	41100.0
88	46537.0
89	29764.0
90	12991.0
91	7663.5
92	2336.0
93	1561.0
94	786.0
95	448.0
96	110.0
97	66.0
98	22.0
99	14.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025537936338901432
2	6.453387440206733E-4
3	0.0012503438165400544
4	0.028040864731509393
5	0.0021802590205976216
6	0.0020480542084544977
7	0.0
8	0.0
9	0.0
10	9.075076087790717E-4
11	0.0014856235669642582
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.012754403232519695
18	0.0
19	0.0
20	0.0
21	2.912987386204428E-5
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	4.48151905569912E-6
33	3.7868836020657563E-4
34	0.0013265296404869394
35	9.904157113095054E-4
36	1.6133468600516832E-4
37	8.223587467207884E-4
38	0.0
39	6.72227858354868E-5
40	1.5461240742161962E-4
41	0.018618470916901993
42	0.024267425686610732
43	8.066734300258416E-5
44	1.792607622279648E-5
45	5.355415271560448E-4
46	8.828592539727267E-4
47	0.010571903452394223
48	0.005673603124515086
49	0.0
50	0.013345963747871979
51	0.11400536325792991
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.4627725E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	36.275010231719705
#Duplication Level	Relative count
1	100.0
2	15.76307756698442
3	5.774407008378014
4	3.0733815856888227
5	2.0068265296549934
6	1.3985004726614085
7	1.0189281049524812
8	0.821204095917793
9	0.7187339890458012
10++	13.749034832620131
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112625	0.2523655418240567	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	21703305	5.6395273	7.1414924	18
AAAAA	11542230	3.7668424	4.001372	47
TGTGT	8004095	3.2440538	37.948574	4
TGTTT	7495235	2.432381	30.486603	6
CTTTG	5505340	2.3209326	38.922127	1
GTTTG	5624640	2.2796624	37.24086	7
TTTGA	6373005	2.1646354	31.45834	8
GTGTT	5303340	2.1494398	37.242638	5
GAGGG	3153915	2.086791	10.2374935	11
TTTGT	6343610	2.0586514	30.240202	2
TTGTG	4881020	1.9782737	37.24923	3
TGAGG	3391410	1.7967176	13.2688675	10
TTGAG	4004000	1.6984941	22.0814	9
AGGGG	2359125	1.5609174	5.7156796	12
TGAGC	2554150	1.4075003	5.3198676	10
TGAGT	2737390	1.1611991	6.2966123	10
TTGAT	3164995	1.0750124	8.239599	9
TGATT	2961020	1.0057311	5.1362824	10
TTGAC	2013660	0.88850254	6.204885	9
>>END_MODULE