FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_3_ACAGTG_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_3_ACAGTG_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29275164
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48459 0.16552938866542302 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34493 0.11782342192856717 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 15127995 5.240991 6.710867 17
TGTGT 6838425 3.9682212 33.406887 4
AAAAA 7551800 3.5464687 3.7683966 47
GTTTG 4939685 2.8664148 32.72591 7
TGTTT 6250565 2.8025618 25.95726 6
GTGTT 4695645 2.7248025 32.822052 5
TTTGA 5367030 2.5573647 27.087662 8
CTTTG 4047300 2.524663 35.443016 1
TTGTG 4328960 2.5120215 32.807 3
GGGGG 1977055 2.486967 5.3467803 13
GAGGG 2248345 2.322379 10.576637 11
TTTGT 4753505 2.131326 25.738682 2
TTGAG 3368185 2.077105 20.655539 9
TGAGG 2495875 1.9919991 12.698647 10
AGGGG 1658920 1.7135454 5.9576216 12
AACTT 2852565 1.5527987 14.58747 2
ACTTT 2987265 1.5301383 13.340598 3
TGAGC 1749535 1.5010227 5.2169113 10
GAGTG 1576005 1.2578356 5.0182834 11
TGAGT 2023405 1.247801 6.104101 10
TAACT 2092805 1.139222 14.295946 1
TTGAT 2338830 1.1144415 6.5352187 9
TTGAC 1465860 0.9717472 5.4746118 9
Produced by FastQC (version 0.10.1)