##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_3_ACAGTG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29275164	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.533554312454065	34.0	31.0	34.0	31.0	34.0
2	32.70376425559905	34.0	31.0	34.0	31.0	34.0
3	32.74839129850818	34.0	31.0	34.0	31.0	34.0
4	36.12379534406708	37.0	37.0	37.0	35.0	37.0
5	36.14452854986568	37.0	37.0	37.0	35.0	37.0
6	36.161331666664616	37.0	37.0	37.0	35.0	37.0
7	36.189266335109174	37.0	37.0	37.0	35.0	37.0
8	36.18044609417047	37.0	37.0	37.0	35.0	37.0
9	37.94822191260825	39.0	39.0	39.0	37.0	39.0
10	37.935254470308	39.0	39.0	39.0	37.0	39.0
11	37.936771899894396	39.0	39.0	39.0	37.0	39.0
12	37.877473888788465	39.0	39.0	39.0	37.0	39.0
13	37.88511705690188	39.0	39.0	39.0	37.0	39.0
14	39.432140533866864	41.0	40.0	41.0	37.0	41.0
15	39.38134361945846	41.0	40.0	41.0	37.0	41.0
16	39.35119536819674	41.0	40.0	41.0	37.0	41.0
17	39.322820121520074	41.0	40.0	41.0	37.0	41.0
18	39.290605408734855	41.0	40.0	41.0	37.0	41.0
19	39.27952338712774	41.0	40.0	41.0	37.0	41.0
20	39.24569146734754	41.0	40.0	41.0	36.0	41.0
21	39.22398105096866	41.0	40.0	41.0	36.0	41.0
22	39.186939311424524	41.0	39.0	41.0	36.0	41.0
23	39.14254622109034	41.0	39.0	41.0	36.0	41.0
24	39.102308393558445	41.0	39.0	41.0	36.0	41.0
25	39.072823298274265	41.0	39.0	41.0	36.0	41.0
26	39.01130275478559	41.0	39.0	41.0	36.0	41.0
27	38.94304130969172	41.0	39.0	41.0	36.0	41.0
28	38.90079300665916	41.0	39.0	41.0	35.0	41.0
29	38.81051019902058	41.0	39.0	41.0	35.0	41.0
30	38.77456102380844	40.0	39.0	41.0	35.0	41.0
31	38.73767702889726	40.0	39.0	41.0	35.0	41.0
32	38.66820424985493	40.0	39.0	41.0	35.0	41.0
33	38.60990449105597	40.0	38.0	41.0	35.0	41.0
34	38.566708763783524	40.0	38.0	41.0	35.0	41.0
35	38.51646207686488	40.0	38.0	41.0	35.0	41.0
36	38.43446352683114	40.0	38.0	41.0	34.0	41.0
37	38.37455943884721	40.0	38.0	41.0	34.0	41.0
38	38.318557054027096	40.0	38.0	41.0	34.0	41.0
39	38.25554391428857	40.0	38.0	41.0	34.0	41.0
40	38.18192181604858	40.0	38.0	41.0	34.0	41.0
41	38.33088808657058	40.0	38.0	41.0	34.0	41.0
42	38.36598934168225	40.0	38.0	41.0	34.0	41.0
43	38.34978229327768	40.0	38.0	41.0	34.0	41.0
44	38.31555775400609	40.0	38.0	41.0	34.0	41.0
45	38.27577317756444	40.0	38.0	41.0	34.0	41.0
46	38.178667487567274	40.0	38.0	41.0	34.0	41.0
47	38.14459000127207	40.0	38.0	41.0	34.0	41.0
48	38.071734149806986	40.0	38.0	41.0	33.0	41.0
49	38.01384084475154	40.0	38.0	41.0	33.0	41.0
50	37.93330599275208	40.0	38.0	41.0	33.0	41.0
51	36.41806095432975	38.0	36.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	16.0
6	320.0
7	1697.0
8	6537.0
9	11010.0
10	12237.0
11	14606.0
12	19378.0
13	25592.0
14	31216.0
15	35116.0
16	37544.0
17	38970.0
18	40093.0
19	41009.0
20	43865.0
21	46535.0
22	50648.0
23	56966.0
24	63627.0
25	72122.0
26	84021.0
27	98408.0
28	116755.0
29	140527.0
30	173124.0
31	216024.0
32	280424.0
33	379951.0
34	550272.0
35	854200.0
36	1363900.0
37	2221701.0
38	5211953.0
39	1.6929992E7
40	4803.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.207211676941096	27.10655760776947	30.984187042904466	22.702043672384967
2	21.352918460792484	29.076204609847984	31.43959841413824	18.131278515221297
3	22.615183089399967	27.783688684618152	30.207684283651382	19.393443942330503
4	21.24807240711855	25.60492766872117	31.935877175182238	21.211122748978042
5	26.354615675292294	25.589922025401147	28.8509842046443	19.204478094662264
6	20.59808488062972	25.239075660420912	34.34307284682973	19.819766612119636
7	24.60414953558935	25.52530349384115	30.684389571239073	19.186157399330426
8	22.2601716290963	25.804430255814836	32.511253073321214	19.424145041767655
9	22.499401886230306	26.058649415208208	32.03201838039571	19.409930318165777
10	22.24635661760863	27.720026560056603	30.64104865082824	19.39256817150653
11	25.476212016398097	25.75000676466641	29.03766880575896	19.73611241317653
12	21.26668113678557	29.698580909084484	29.17676833229169	19.857969621838258
13	23.651607251045522	26.153725898442808	29.89573241587571	20.298934434635964
14	24.268769818420026	26.614390765746993	28.584754720961836	20.532084694871145
15	23.2508675987484	28.078846980726297	28.051337564652506	20.618947855872797
16	23.698012795588316	26.95061218998807	28.526856489585743	20.824518524837867
17	23.429691968800455	27.026200082017766	28.689275126566464	20.854832822615315
18	23.19713414739539	27.002224224846678	28.85265126959536	20.947990358162574
19	22.89037202740292	27.258320808897775	29.041982390786508	20.8093247729128
20	22.53667607630246	27.309612178830378	29.145329768969354	21.008381975897812
21	22.61665588479012	27.41605525148667	29.021285262082262	20.94600360164094
22	22.306829689077123	27.593720327777415	29.1942013277852	20.905248655360257
23	22.300934924218875	27.66120013253584	29.056413129246355	20.981451813998927
24	22.13673107391964	27.734393702743176	29.025883344897547	21.10299187843964
25	22.189624237004914	27.84731025230642	28.910560326127577	21.05250518456108
26	22.168901324054787	27.816765780746504	28.901327471040837	21.113005424157873
27	22.19034105558316	27.836403517359848	28.77755004539555	21.19570538166144
28	22.161435358072314	27.86787619314512	28.797026064565074	21.173662384217494
29	22.22722872959906	27.865369051986296	28.693950201907032	21.213452016507613
30	22.2519128444803	27.836164626789017	28.62019251633662	21.291730012394066
31	22.29830077728039	27.787000237402708	28.579105487076923	21.335593498239973
32	22.326587161490856	27.85993052971011	28.467236997107538	21.346245311691504
33	22.307105137638636	27.792809162968435	28.427885187273638	21.472200512119294
34	22.341981815163365	27.80847541265508	28.39093192871738	21.45861084346418
35	22.376118476584576	27.7589635707399	28.347855634207132	21.51706231846839
36	22.34440623506162	27.81751970367377	28.342103787901017	21.49597027336359
37	22.394088245500328	27.744266928714655	28.35628354356653	21.505361282218487
38	22.328551969413944	27.80919157246373	28.389325913643574	21.47293054447875
39	22.326055622678567	27.678416587506156	28.397929950907503	21.597597838907777
40	22.347920778336942	27.761949851128193	28.355564585440955	21.53456478509391
41	22.33437267255225	27.77263675318086	28.32411257307539	21.568878001191504
42	22.252834412037206	27.701144807069067	28.34927251292528	21.69674826796845
43	22.293693894322654	27.802452734128853	28.287253414673618	21.616599956874868
44	22.33276731700128	27.759937699714456	28.246101842355493	21.661193140928773
45	22.33845199381101	27.738353535695165	28.21348397903341	21.709710491460417
46	22.24230311117593	27.904750456219375	28.1216848793355	21.731261553269192
47	22.30850820854205	27.78986310014228	28.162968553467714	21.738660137847955
48	22.25460650701637	27.758088264771143	28.12745503027897	21.85985019793352
49	22.308406826052916	27.82206628677043	28.119219838868343	21.750307048308308
50	22.296469769567267	27.78468632800871	28.14440487750026	21.774439024923755
51	22.312938361257984	27.72600960421227	28.160888937680006	21.800163096849744
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.90925534932607
2	39.48419697601378
3	42.00862703173047
4	42.45919515609659
5	45.55909376995456
6	40.41785149274935
7	43.790306934919776
8	41.68431667086395
9	41.90933220439608
10	41.63892478911516
11	45.21232442957463
12	41.12465075862383
13	43.95054168568148
14	44.80085451329116
15	43.8698154546212
16	44.52253132042618
17	44.28452479141577
18	44.145124505557966
19	43.69969680031572
20	43.54505805220027
21	43.56265948643106
22	43.21207834443738
23	43.2823867382178
24	43.23972295235928
25	43.242129421566
26	43.281906748212656
27	43.3860464372446
28	43.335097742289804
29	43.44068074610667
30	43.543642856874364
31	43.63389427552036
32	43.67283247318236
33	43.77930564975793
34	43.800592658627544
35	43.89318079505297
36	43.84037650842521
37	43.899449527718815
38	43.801482513892694
39	43.92365346158634
40	43.88248556343085
41	43.903250673743756
42	43.949582680005655
43	43.91029385119752
44	43.99396045793005
45	44.048162485271426
46	43.97356466444512
47	44.04716834639
48	44.11445670494989
49	44.058713874361224
50	44.070908794491025
51	44.11310145810772
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29866.0
1	18020.0
2	6174.0
3	8055.5
4	9937.0
5	49065.5
6	88194.0
7	97537.0
8	106880.0
9	68343.0
10	29806.0
11	31835.0
12	33864.0
13	40399.0
14	46934.0
15	64302.5
16	81671.0
17	118315.0
18	154959.0
19	193157.5
20	231356.0
21	272878.5
22	314401.0
23	376556.5
24	438712.0
25	518645.0
26	698416.5
27	798255.0
28	874314.0
29	950373.0
30	1015438.0
31	1080503.0
32	1167978.5
33	1255454.0
34	1332249.0
35	1409044.0
36	1538795.5
37	1668547.0
38	1740487.5
39	1812428.0
40	1884207.0
41	1955986.0
42	1977785.0
43	1999584.0
44	2005962.0
45	2012340.0
46	1981388.0
47	1950436.0
48	1903268.0
49	1856100.0
50	1774133.0
51	1692166.0
52	1576807.0
53	1461448.0
54	1347055.5
55	1232663.0
56	1117830.5
57	1002998.0
58	892805.0
59	782612.0
60	689137.0
61	595662.0
62	512424.5
63	429187.0
64	367995.0
65	306803.0
66	256067.0
67	205331.0
68	185088.5
69	164846.0
70	144960.0
71	125074.0
72	105123.0
73	85172.0
74	71451.0
75	49437.0
76	41144.0
77	38234.0
78	35324.0
79	35570.0
80	35816.0
81	31635.5
82	27455.0
83	24886.0
84	22317.0
85	19591.5
86	16866.0
87	19296.0
88	21726.0
89	14698.0
90	7670.0
91	5028.0
92	2386.0
93	1804.5
94	1223.0
95	970.5
96	718.0
97	531.5
98	345.0
99	222.5
100	100.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004085374210030045
2	7.82233021820134E-4
3	0.0054517200996721995
4	0.00991967115880205
5	0.013543903631077867
6	4.167354963408574E-4
7	0.02793494171373387
8	0.007760844653167442
9	0.013584894007767129
10	0.003859927138239089
11	0.01854131372244405
12	0.01930305155591955
13	0.014107521310555255
14	0.0064628160580073955
15	0.005298006187087458
16	0.06005090184977273
17	0.0034807661538633914
18	8.403027221299256E-4
19	0.003610569013379396
20	0.0019231318396713338
21	0.0024935812485969338
22	0.003935076162169408
23	2.1861534234274486E-4
24	0.04753175763592648
25	0.005847960407668425
26	0.01140557231378789
27	0.0385412016820811
28	5.4653835585686214E-5
29	0.0983188343539254
30	0.03810055513267151
31	4.782210613747543E-5
32	0.013479002201319864
33	0.010514031620796385
34	0.022930699892919473
35	0.015149360051407398
36	0.009670313033942356
37	0.01778640761841676
38	0.054923688898890544
39	0.018855573277061742
40	0.03204422697683265
41	0.02133890693148636
42	0.019736866375880936
43	0.023388425765949593
44	0.009379964532393396
45	0.013096425352220059
46	0.032922104210927734
47	0.02035513789094401
48	0.051535151092577995
49	0.007115246220311525
50	0.05716108029317957
51	0.11910778706483079
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9275164E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.704314580178053
#Duplication Level	Relative count
1	100.0
2	10.273096670551547
3	2.9640595961823166
4	1.445805902919961
5	0.9336315242385107
6	0.6432103820833465
7	0.5027246668976596
8	0.39499795256244685
9	0.301760796295713
10++	8.536869625476424
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48459	0.16552938866542302	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34493	0.11782342192856717	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	15127995	5.240991	6.710867	17
TGTGT	6838425	3.9682212	33.406887	4
AAAAA	7551800	3.5464687	3.7683966	47
GTTTG	4939685	2.8664148	32.72591	7
TGTTT	6250565	2.8025618	25.95726	6
GTGTT	4695645	2.7248025	32.822052	5
TTTGA	5367030	2.5573647	27.087662	8
CTTTG	4047300	2.524663	35.443016	1
TTGTG	4328960	2.5120215	32.807	3
GGGGG	1977055	2.486967	5.3467803	13
GAGGG	2248345	2.322379	10.576637	11
TTTGT	4753505	2.131326	25.738682	2
TTGAG	3368185	2.077105	20.655539	9
TGAGG	2495875	1.9919991	12.698647	10
AGGGG	1658920	1.7135454	5.9576216	12
AACTT	2852565	1.5527987	14.58747	2
ACTTT	2987265	1.5301383	13.340598	3
TGAGC	1749535	1.5010227	5.2169113	10
GAGTG	1576005	1.2578356	5.0182834	11
TGAGT	2023405	1.247801	6.104101	10
TAACT	2092805	1.139222	14.295946	1
TTGAT	2338830	1.1144415	6.5352187	9
TTGAC	1465860	0.9717472	5.4746118	9
>>END_MODULE