FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_3_ACAGTG_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_3_ACAGTG_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29275164
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58490 0.19979392771292417 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 13713115 4.9768157 6.1944494 16
AAAAA 7574750 3.4290724 3.6544847 47
TGTGT 5427015 3.3205366 35.608963 4
TGTTT 5094470 2.4006584 27.521038 6
GTTTG 3771435 2.307565 34.84878 7
CTTTG 3529480 2.254184 36.431866 1
GTGTT 3508235 2.146525 34.82546 5
GAGGG 1972425 2.1265626 10.547272 11
TTTGA 4229500 2.0831423 28.305283 8
TTTGT 4207440 1.9826647 27.216198 2
TTGTG 3174770 1.9424934 34.813103 3
TGAGG 2182325 1.8120961 13.104851 10
TTGAG 2673205 1.7095356 21.161634 9
AGGGG 1472455 1.5875218 5.9487576 12
TGAGC 1661760 1.4403281 5.2454877 10
GAGTG 1464465 1.21602 5.007458 11
TGAGT 1864670 1.192471 6.2322745 10
TTGAT 2106380 1.0374488 7.194327 9
TTGAC 1329925 0.887778 5.6463776 9
Produced by FastQC (version 0.10.1)