##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_3_ACAGTG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29275164	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05894501564534	34.0	33.0	34.0	31.0	34.0
2	33.23177608842772	34.0	34.0	34.0	31.0	34.0
3	33.269333111165494	34.0	34.0	34.0	31.0	34.0
4	36.575201423295184	37.0	37.0	37.0	35.0	37.0
5	36.517977866836205	37.0	37.0	37.0	35.0	37.0
6	36.500452260489475	37.0	37.0	37.0	35.0	37.0
7	36.502586595245035	37.0	37.0	37.0	35.0	37.0
8	36.486307711205306	37.0	37.0	37.0	35.0	37.0
9	38.312448941362035	39.0	39.0	39.0	37.0	39.0
10	38.28232709473463	39.0	39.0	39.0	37.0	39.0
11	38.26638815755225	39.0	39.0	39.0	37.0	39.0
12	38.24908420666747	39.0	39.0	39.0	37.0	39.0
13	38.26232095574255	39.0	39.0	39.0	37.0	39.0
14	39.85192574839205	41.0	40.0	41.0	38.0	41.0
15	39.82935473905458	41.0	40.0	41.0	38.0	41.0
16	39.79100574808052	41.0	40.0	41.0	38.0	41.0
17	39.74679728523468	41.0	40.0	41.0	38.0	41.0
18	39.73215319989326	41.0	40.0	41.0	38.0	41.0
19	39.74452559855856	41.0	40.0	41.0	38.0	41.0
20	39.718487418208824	41.0	40.0	41.0	38.0	41.0
21	39.676297184876574	41.0	40.0	41.0	37.0	41.0
22	39.6502056145612	41.0	40.0	41.0	37.0	41.0
23	39.60891392444462	41.0	40.0	41.0	37.0	41.0
24	39.59227821917582	41.0	40.0	41.0	37.0	41.0
25	39.5475463433783	41.0	40.0	41.0	37.0	41.0
26	39.46779591055408	41.0	39.0	41.0	37.0	41.0
27	39.413631602542004	41.0	39.0	41.0	37.0	41.0
28	39.36258263147561	41.0	39.0	41.0	36.0	41.0
29	39.30230173945396	41.0	39.0	41.0	36.0	41.0
30	39.2429855559477	40.0	39.0	41.0	36.0	41.0
31	39.17763388106041	40.0	39.0	41.0	36.0	41.0
32	39.10610536630982	40.0	39.0	41.0	36.0	41.0
33	39.01499489464858	40.0	39.0	41.0	35.0	41.0
34	39.12816546476051	40.0	39.0	41.0	36.0	41.0
35	39.152315662518575	40.0	39.0	41.0	36.0	41.0
36	39.09993771512262	40.0	39.0	41.0	35.0	41.0
37	39.08097276585709	40.0	39.0	41.0	35.0	41.0
38	39.05461926703468	40.0	39.0	41.0	35.0	41.0
39	39.0262842934031	40.0	39.0	41.0	35.0	41.0
40	38.831047778246436	40.0	39.0	41.0	35.0	41.0
41	38.85155013990699	40.0	39.0	41.0	35.0	41.0
42	38.81247619996253	40.0	38.0	41.0	35.0	41.0
43	38.56662664639556	40.0	38.0	41.0	35.0	41.0
44	38.634802694871325	40.0	38.0	41.0	35.0	41.0
45	38.64403307868745	40.0	38.0	41.0	35.0	41.0
46	38.61280158840442	40.0	38.0	41.0	35.0	41.0
47	38.56567307359918	40.0	38.0	41.0	35.0	41.0
48	38.5055473301533	40.0	38.0	41.0	35.0	41.0
49	38.45153834151023	40.0	38.0	41.0	35.0	41.0
50	38.502606783005554	40.0	38.0	41.0	35.0	41.0
51	38.21950684887709	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	0.0
10	3.0
11	13.0
12	37.0
13	87.0
14	165.0
15	297.0
16	493.0
17	761.0
18	1350.0
19	2220.0
20	4054.0
21	7087.0
22	11633.0
23	18609.0
24	27314.0
25	38607.0
26	53120.0
27	70502.0
28	93187.0
29	120767.0
30	156471.0
31	201326.0
32	262468.0
33	353706.0
34	514763.0
35	813831.0
36	1291449.0
37	2145170.0
38	5063761.0
39	1.8004702E7
40	17208.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.69753707619789	27.999409130370918	28.63847228969396	23.664581503737235
2	21.442127084486476	27.649964085490332	31.40697909716784	19.500929732855347
3	20.490682156211292	26.566823598053425	32.349485583423345	20.59300866231194
4	21.20750063021775	26.506193085595324	31.973280817921477	20.31302546626544
5	25.253327359669104	26.61808487221455	28.43613446537823	19.692453302738116
6	21.442333222625102	26.320836146726357	31.72524928908133	20.51158134156721
7	25.320944401882773	26.728864781082013	27.95872979567254	19.99146102136268
8	21.534779446496014	26.8419435669088	31.289010029115467	20.334266957479727
9	20.8935601522164	27.1035748937222	31.696737890178856	20.306127063882546
10	21.388747813675785	26.79399848964125	31.529145319220074	20.28810837746289
11	24.95286357235181	26.669095121664537	28.014349654957964	20.36369165102569
12	21.28299947354693	30.535791362261882	27.727445011068085	20.453764153123107
13	23.38312006143384	27.089167435324068	28.658403595332015	20.869308907910078
14	22.680747407597785	27.360649456993645	28.94829214278697	21.010310992621594
15	22.885063940205423	27.304253530398668	28.692911848418678	21.117770680977227
16	22.809696300932764	27.299566963997197	28.643702901203216	21.247033833866823
17	22.826805816143096	27.436644686094763	28.4991961502124	21.237353347549746
18	22.615453153396512	27.406633827909555	28.73139156453573	21.246521454158206
19	22.297207284645783	27.609946096288308	28.985572890385857	21.107273728680052
20	22.01383442780052	27.652230047918625	29.056260912760973	21.27767461151989
21	22.149146628179437	27.67165027666455	28.87110043175164	21.30810266340438
22	22.056060898582842	27.72404964153232	28.92763299293558	21.292256466949254
23	22.143544610031903	27.75239448701295	28.79620076594618	21.30786013700897
24	21.97649516156425	27.8203633632932	28.792969357917176	21.410172117225372
25	22.059859340156045	27.916355993770008	28.679897403819837	21.343887262254107
26	22.042925741723504	27.877971362821878	28.663907262542466	21.415195632912145
27	22.073199045894295	27.8698089571696	28.553798317023887	21.503193679912215
28	22.064894324759376	27.898955578865415	28.582008968421153	21.454141127954056
29	22.104631079094894	27.91386924425086	28.511430371491684	21.47006930516256
30	22.096128991796597	27.87162524520785	28.485278511163937	21.54696725183162
31	22.20717875397726	27.82715410236472	28.402433544010208	21.56323359964781
32	22.187072974211176	27.90222114554166	28.341200752965896	21.569505127281268
33	22.180381295216655	27.84850325689038	28.29079283723227	21.680322610660696
34	22.16216125738809	27.90205240501652	28.28331376657845	21.652472571016936
35	22.183752896486197	27.880006947980156	28.248150834705537	21.68808932082811
36	22.235057916387333	27.913173685216947	28.197162137669462	21.654606260726254
37	22.29510652784046	27.823280511767585	28.20951916785163	21.672093792540327
38	22.215052322166326	27.851717585595765	28.26603464971195	21.66719544252596
39	22.197269330412635	27.727936895588357	28.282078966321077	21.792714807677935
40	22.269251847743703	27.967368517559798	28.138745866632892	21.624633768063607
41	22.22616050838234	27.77844257232379	28.248759567960143	21.74663735133373
42	22.17695695494559	27.834410128965075	28.183699864712057	21.804933051377272
43	22.323566415641498	27.92401214518032	28.037921032826745	21.714500406351434
44	22.249017785110805	27.79998962943399	28.156725486267163	21.79426709918804
45	22.24383781419636	27.75425954915231	28.14723087460757	21.854671762043758
46	22.16362647874492	27.887655898358076	28.115709274933526	21.83300834796348
47	22.22439830734056	27.865852858658457	28.067388402702964	21.842360431298022
48	22.15907654693241	27.817893693097673	28.070141639513956	21.95288812045596
49	22.266003373850626	27.831357340379583	28.045965939377602	21.85667334639219
50	22.208654598374572	27.81663552231974	28.05259238255962	21.922117496746072
51	22.231998643205173	27.791351199212468	28.046916695383622	21.92973346219874
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.36211857993512
2	40.94305681734182
3	41.08369081852323
4	41.520526096483195
5	44.945780662407216
6	41.95391456419231
7	45.31240542324545
8	41.86904640397574
9	41.199687216098944
10	41.676856191138675
11	45.3165552233775
12	41.73676362667003
13	44.25242896934392
14	43.691058400219376
15	44.00283462118265
16	44.05673013479959
17	44.06415916369284
18	43.861974607554714
19	43.404481013325835
20	43.29150903932041
21	43.457249291583814
22	43.3483173655321
23	43.451404747040876
24	43.38666727878962
25	43.40374660241015
26	43.45812137463565
27	43.576392725806514
28	43.51903545271343
29	43.574700384257454
30	43.643096243628214
31	43.77041235362507
32	43.756578101492444
33	43.86070390587735
34	43.81463382840502
35	43.8718422173143
36	43.88966417711359
37	43.96720032038078
38	43.88224776469228
39	43.98998413809056
40	43.89388561580731
41	43.97279785971607
42	43.981890006322864
43	44.038066821992935
44	44.04328488429884
45	44.09850957624012
46	43.9966348267084
47	44.066758738638576
48	44.11196466738837
49	44.122676720242815
50	44.13077209512064
51	44.161732105403914
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43994.0
1	25873.0
2	7752.0
3	9822.0
4	11892.0
5	35019.5
6	58147.0
7	78313.5
8	98480.0
9	61576.0
10	24672.0
11	28680.0
12	32688.0
13	39676.5
14	46665.0
15	65650.0
16	84635.0
17	123357.0
18	162079.0
19	201301.0
20	240523.0
21	281105.5
22	321688.0
23	384621.5
24	447555.0
25	528699.0
26	711948.5
27	814054.0
28	881958.0
29	949862.0
30	1012633.0
31	1075404.0
32	1158414.0
33	1241424.0
34	1318156.0
35	1394888.0
36	1522204.5
37	1649521.0
38	1719254.0
39	1788987.0
40	1859697.5
41	1930408.0
42	1948739.0
43	1967070.0
44	1978124.0
45	1989178.0
46	1960908.5
47	1932639.0
48	1892511.5
49	1852384.0
50	1774823.0
51	1697262.0
52	1589508.0
53	1481754.0
54	1367605.5
55	1253457.0
56	1138336.0
57	1023215.0
58	910457.5
59	797700.0
60	703788.0
61	609876.0
62	524538.5
63	439201.0
64	378090.5
65	316980.0
66	264366.5
67	211753.0
68	191732.5
69	171712.0
70	151146.5
71	130581.0
72	110332.5
73	90084.0
74	75263.5
75	50890.5
76	41338.0
77	38801.0
78	36264.0
79	37181.0
80	38098.0
81	33028.0
82	27958.0
83	25035.5
84	22113.0
85	19860.0
86	17607.0
87	20560.5
88	23514.0
89	14874.5
90	6235.0
91	3673.5
92	1112.0
93	756.5
94	401.0
95	228.0
96	55.0
97	34.0
98	13.0
99	9.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03356087091433544
2	3.005960957212742E-4
3	0.0041810184223049955
4	0.052727287881290774
5	0.0
6	5.1237970861580824E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	1.195552653436886E-4
12	0.0
13	3.176754193418011E-4
14	0.0
15	0.0
16	0.0
17	0.016293674733982706
18	0.0
19	0.0
20	0.003245071487900119
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.0247594172316165E-5
27	3.4158647241053884E-6
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.003484182018587496
35	0.001602040555605427
36	1.0247594172316165E-4
37	0.0
38	0.0
39	0.0
40	0.0
41	0.004409881358820056
42	0.006985443360795519
43	4.099037668926466E-5
44	6.831729448210777E-6
45	0.0
46	0.0
47	0.008239065714542197
48	0.0
49	3.07427825169485E-5
50	0.009783036569837832
51	0.14197016966326814
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9275164E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.697614046031205
#Duplication Level	Relative count
1	100.0
2	10.659231911087428
3	3.096334640124843
4	1.466017077100699
5	0.9585252286885142
6	0.6914576434616019
7	0.5170073205699134
8	0.40726220835077837
9	0.3260635126048288
10++	8.751062561057614
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58490	0.19979392771292417	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13713115	4.9768157	6.1944494	16
AAAAA	7574750	3.4290724	3.6544847	47
TGTGT	5427015	3.3205366	35.608963	4
TGTTT	5094470	2.4006584	27.521038	6
GTTTG	3771435	2.307565	34.84878	7
CTTTG	3529480	2.254184	36.431866	1
GTGTT	3508235	2.146525	34.82546	5
GAGGG	1972425	2.1265626	10.547272	11
TTTGA	4229500	2.0831423	28.305283	8
TTTGT	4207440	1.9826647	27.216198	2
TTGTG	3174770	1.9424934	34.813103	3
TGAGG	2182325	1.8120961	13.104851	10
TTGAG	2673205	1.7095356	21.161634	9
AGGGG	1472455	1.5875218	5.9487576	12
TGAGC	1661760	1.4403281	5.2454877	10
GAGTG	1464465	1.21602	5.007458	11
TGAGT	1864670	1.192471	6.2322745	10
TTGAT	2106380	1.0374488	7.194327	9
TTGAC	1329925	0.887778	5.6463776	9
>>END_MODULE