FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_1_CGATGT_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_1_CGATGT_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24596630
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50793 0.20650389911138234 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33711 0.13705536083601697 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 13129140 5.8676686 7.5431147 18
AAAAA 6192525 3.6667833 3.9353468 47
TGTGT 5061035 3.589233 31.303028 4
TGTTT 4772030 2.6865752 25.047045 6
GTTTG 3746125 2.6567125 30.732166 7
GTGTT 3542175 2.5120733 30.793987 5
TTTGA 4111255 2.4485438 26.03405 8
CTTTG 3230485 2.4281723 32.84628 1
TTGTG 3314090 2.350318 30.75568 3
GAGGG 1842755 2.193818 9.268312 11
TTTGT 3710975 2.0892184 24.819674 2
TTGAG 2577115 1.9334536 18.993214 9
TGAGG 2028800 1.9173691 11.5638485 10
AGGGG 1374240 1.6360463 5.179037 12
ACTTT 2386620 1.5064898 12.077374 3
AACTT 2238585 1.494838 13.118069 2
TGAGT 1582360 1.1871492 5.38544 10
TTGAT 1874615 1.1164662 6.5887246 9
TAACT 1636365 1.0926993 12.869976 1
TTGAC 1191750 0.94762063 5.171499 9
Produced by FastQC (version 0.10.1)