##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_1_CGATGT_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	24596630	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.465708635695215	34.0	31.0	34.0	31.0	34.0
2	32.62849077292296	34.0	31.0	34.0	31.0	34.0
3	32.70270045124068	34.0	31.0	34.0	31.0	34.0
4	36.08433415471957	37.0	37.0	37.0	35.0	37.0
5	36.10938754618011	37.0	37.0	37.0	35.0	37.0
6	36.10666469349663	37.0	37.0	37.0	35.0	37.0
7	36.119360294479364	37.0	37.0	37.0	35.0	37.0
8	36.12651245312874	37.0	37.0	37.0	35.0	37.0
9	37.89386379353594	39.0	39.0	39.0	37.0	39.0
10	37.88660373392615	39.0	39.0	39.0	35.0	39.0
11	37.86554954886096	39.0	39.0	39.0	35.0	39.0
12	37.822083838314434	39.0	38.0	39.0	35.0	39.0
13	37.817862894225755	39.0	38.0	39.0	35.0	39.0
14	39.34228408525883	41.0	40.0	41.0	37.0	41.0
15	39.30381849058184	41.0	40.0	41.0	37.0	41.0
16	39.2660171738974	41.0	40.0	41.0	36.0	41.0
17	39.23250242004698	41.0	40.0	41.0	36.0	41.0
18	39.19230032732126	41.0	39.0	41.0	36.0	41.0
19	39.20540992810804	41.0	40.0	41.0	36.0	41.0
20	39.16909727064236	41.0	39.0	41.0	36.0	41.0
21	39.13326077596809	41.0	39.0	41.0	36.0	41.0
22	39.06400246700463	41.0	39.0	41.0	36.0	41.0
23	39.03565785231554	41.0	39.0	41.0	36.0	41.0
24	38.9854892316549	41.0	39.0	41.0	36.0	41.0
25	38.95262720949984	41.0	39.0	41.0	36.0	41.0
26	38.886936625057984	41.0	39.0	41.0	35.0	41.0
27	38.82558557818693	41.0	39.0	41.0	35.0	41.0
28	38.766724547224555	40.0	39.0	41.0	35.0	41.0
29	38.68193537082112	40.0	39.0	41.0	35.0	41.0
30	38.62927653097193	40.0	39.0	41.0	35.0	41.0
31	38.59475651745788	40.0	38.0	41.0	35.0	41.0
32	38.51774576435878	40.0	38.0	41.0	35.0	41.0
33	38.45523065558168	40.0	38.0	41.0	34.0	41.0
34	38.40894053372352	40.0	38.0	41.0	34.0	41.0
35	38.34229079349488	40.0	38.0	41.0	34.0	41.0
36	38.28306446858777	40.0	38.0	41.0	34.0	41.0
37	38.22105629917594	40.0	38.0	41.0	34.0	41.0
38	38.13142251601134	40.0	38.0	41.0	34.0	41.0
39	38.06658249524427	40.0	38.0	41.0	33.0	41.0
40	37.97551436924489	40.0	38.0	41.0	33.0	41.0
41	38.17308131235865	40.0	38.0	41.0	34.0	41.0
42	38.20482395352534	40.0	38.0	41.0	34.0	41.0
43	38.17916381227835	40.0	38.0	41.0	34.0	41.0
44	38.138719206655544	40.0	38.0	41.0	34.0	41.0
45	38.0996169800497	40.0	38.0	41.0	33.0	41.0
46	38.037464116019144	40.0	38.0	41.0	33.0	41.0
47	37.974793254197834	40.0	38.0	41.0	33.0	41.0
48	37.88330202145578	40.0	38.0	41.0	33.0	41.0
49	37.826446224543766	40.0	38.0	41.0	33.0	41.0
50	37.71988951331951	40.0	37.0	41.0	33.0	41.0
51	36.241424983829084	38.0	35.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	61.0
5	186.0
6	568.0
7	1709.0
8	5024.0
9	7678.0
10	8911.0
11	11845.0
12	17129.0
13	24329.0
14	30090.0
15	33954.0
16	35692.0
17	36886.0
18	37824.0
19	38829.0
20	40118.0
21	42576.0
22	46737.0
23	51620.0
24	58665.0
25	66043.0
26	76809.0
27	90355.0
28	108006.0
29	130520.0
30	158843.0
31	198137.0
32	255189.0
33	343418.0
34	494509.0
35	759351.0
36	1209428.0
37	1958180.0
38	4546063.0
39	1.3668014E7
40	3331.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.746364495973598	27.03941798107102	29.963226982893527	23.25099054006186
2	22.0059533073487	28.70668475735723	30.634211733611494	18.65315020168258
3	22.57301402807192	27.42698800472862	29.884554411545107	20.11544355565436
4	21.643445271276406	25.581354787073536	31.11644350280809	21.65875643884197
5	26.26398519894738	25.576915438290342	28.343965345820354	19.815134016941915
6	21.273473703605617	25.234010308593486	33.01086419949842	20.48165178830248
7	24.717492969070417	25.653109357566915	29.815440004733716	19.813957668628948
8	22.456195179678627	25.706353007229854	31.702759019957387	20.13469279313413
9	22.555755738106356	25.883403211077034	31.452844945742108	20.107996105074506
10	22.507594902685906	27.30264587781932	30.101033726528037	20.088725492966734
11	25.3914778130381	25.69454389958884	28.54649047837118	20.36748780900188
12	21.619276400225125	29.273810447907035	28.656549714919162	20.450363436948685
13	23.651118686852257	26.077870558920544	29.410310688517765	20.860700065709434
14	24.230095654246405	26.42370798706005	28.288044077081224	21.058152281612323
15	23.304263459515028	27.673770928125734	27.845902670894272	21.176062941464963
16	23.700443456472843	26.701268902298597	28.220176264857233	21.378111376371326
17	23.491389229840696	26.765208289251746	28.371568365476772	21.371834115430786
18	23.29973767977872	26.71567414654242	28.49435056239037	21.49023761128849
19	23.009254537768395	26.91874656697914	28.698004932001588	21.37399396325088
20	22.7497895084481	26.962405552220098	28.744150623847396	21.5436543154844
21	22.815033572458784	27.053572248990037	28.59666203410402	21.534732144447158
22	22.571562051242676	27.217385773673076	28.77512065004298	21.43593152504127
23	22.565834855897123	27.275497542075378	28.650863449409268	21.507804152618228
24	22.43285555056687	27.325301345960025	28.594717312925273	21.64712579054783
25	22.454548803422828	27.474699323854267	28.49508192961959	21.57566994310331
26	22.45475913307124	27.435134929942954	28.47537608498154	21.634729852004266
27	22.464876324818075	27.420736301312044	28.37921596083136	21.735171413038522
28	22.467357676141976	27.48130677207637	28.345320031859718	21.70601551992194
29	22.502728783459386	27.473537534413435	28.25975186604194	21.76398181608524
30	22.527111055641523	27.42823845606292	28.21457683858434	21.830073649711224
31	22.596608220238764	27.39471753604305	28.137205144847265	21.871469098870925
32	22.640861747479388	27.467548115786588	28.040197203497737	21.851392933236287
33	22.598228541541335	27.382781699020427	28.035810631735757	21.98317912770248
34	22.63166373060044	27.42836508795002	27.97591429313804	21.964056888311504
35	22.683626149005917	27.376982834346954	27.93392431446342	22.00546670218371
36	22.671983108136406	27.37268381567512	27.929249544446172	22.026083531742305
37	22.69623514492026	27.346383078666303	27.938573481323736	22.018808295089702
38	22.653832424724992	27.398401437199947	27.978826941837138	21.968939196237926
39	22.623885043554015	27.2995656472237	27.98578903246228	22.09076027676001
40	22.67652760351126	27.348275925891652	27.95031321193197	22.024883258665117
41	22.676441217490126	27.341796199769536	27.930797683079472	22.050964899660862
42	22.595050055877415	27.30815200364804	27.941989138414364	22.154808802060174
43	22.62792580749757	27.401692428810886	27.862532174547972	22.10784958914357
44	22.675276896816797	27.339297047897855	27.86860287594678	22.11682317933857
45	22.678533073428444	27.33482971022465	27.8190094636816	22.167627752665307
46	22.582032663440536	27.472963311655285	27.75047489314269	22.194529131761488
47	22.644569067041896	27.3759495555803	27.79391381706806	22.185567560309746
48	22.605539055058824	27.310495868457764	27.753090648235286	22.330874428248126
49	22.659999485187992	27.38073613318683	27.74312556195736	22.21613881966782
50	22.59894125774647	27.392739059046882	27.775424140054227	22.23289554315242
51	22.645256626679505	27.324237722050388	27.75544885238135	22.275056798888762
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.99735503603546
2	40.65910350903128
3	42.688457583726276
4	43.30220171011837
5	46.0791192158893
6	41.755125491908096
7	44.531450637699365
8	42.59088797281276
9	42.66375184318086
10	42.59632039565264
11	45.75896562203998
12	42.0696398371738
13	44.511818752561695
14	45.28824793585873
15	44.48032640097999
16	45.07855483284417
17	44.86322334527148
18	44.789975291067215
19	44.383248501019274
20	44.293443823932506
21	44.34976571690594
22	44.00749357628395
23	44.07363900851535
24	44.0799813411147
25	44.03021874652614
26	44.089488985075505
27	44.20004773785659
28	44.17337319606391
29	44.26671059954463
30	44.35718470535275
31	44.46807731910969
32	44.492254680715675
33	44.581407669243816
34	44.59572061891194
35	44.68909285118963
36	44.69806663987871
37	44.715043440009964
38	44.62277162096292
39	44.71464532031403
40	44.701410862176374
41	44.727406117150984
42	44.74985885793759
43	44.735775396641145
44	44.792100076155364
45	44.84616082609375
46	44.77656179520203
47	44.83013662735164
48	44.93641348330695
49	44.876138304855814
50	44.83183680089889
51	44.92031342556827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29158.0
1	17512.0
2	5866.0
3	8382.5
4	10899.0
5	41797.5
6	72696.0
7	88198.0
8	103700.0
9	64975.5
10	26251.0
11	27243.0
12	28235.0
13	33021.0
14	37807.0
15	50821.5
16	63836.0
17	90315.5
18	116795.0
19	146461.5
20	176128.0
21	208754.0
22	241380.0
23	289142.0
24	336904.0
25	398186.5
26	536536.0
27	613603.0
28	673403.5
29	733204.0
30	787331.0
31	841458.0
32	911309.5
33	981161.0
34	1047209.0
35	1113257.0
36	1215445.5
37	1317634.0
38	1382887.5
39	1448141.0
40	1515347.5
41	1582554.0
42	1609519.5
43	1636485.0
44	1652472.0
45	1668459.0
46	1657290.0
47	1646121.0
48	1617802.0
49	1589483.0
50	1534071.5
51	1478660.0
52	1389537.0
53	1300414.0
54	1208413.0
55	1116412.0
56	1022192.5
57	927973.0
58	831285.5
59	734598.0
60	653804.5
61	573011.0
62	493923.0
63	414835.0
64	357399.0
65	299963.0
66	251018.5
67	202074.0
68	184250.0
69	166426.0
70	147068.0
71	127710.0
72	108224.5
73	88739.0
74	74606.0
75	51477.0
76	42481.0
77	39144.0
78	35807.0
79	36409.0
80	37011.0
81	32334.0
82	27657.0
83	25280.0
84	22903.0
85	20481.5
86	18060.0
87	21166.0
88	24272.0
89	17051.0
90	9830.0
91	6547.5
92	3265.0
93	2506.0
94	1747.0
95	1362.5
96	978.0
97	740.5
98	503.0
99	323.5
100	144.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00799296488990565
2	0.0022523410727404527
3	8.537754968871751E-5
4	8.293819112618274E-4
5	4.065597604224644E-6
6	2.601982466703772E-4
7	0.028723447073847108
8	0.013546571217276513
9	0.01883184810276855
10	0.01686816445992805
11	0.01749833208858287
12	0.015372024541573378
13	0.0142702475908285
14	0.004764880392151283
15	0.008521492578454854
16	0.045241970139811835
17	0.008464574211995708
18	0.007647389093546555
19	0.018644830612974218
20	0.0025653920882657503
21	0.004764880392151283
22	0.026434515622668634
23	0.017636562407126504
24	0.04917746862070129
25	0.009058151462212507
26	0.021600520071245532
27	0.06731409953314743
28	0.010420126659627762
29	0.08027115909781136
30	0.059325200240846
31	0.0267922882118404
32	0.040025808413591615
33	0.02870718468343021
34	0.030927000975336863
35	0.015209400637404393
36	0.014180804443535557
37	0.015709469142724024
38	0.04490859113626541
39	0.0188562416883939
40	0.054621303812758086
41	0.017612168821501156
42	0.010631537735047443
43	0.04238792062164614
44	0.01950673730506984
45	0.03938344399212412
46	0.03442341491497006
47	0.017628431211918056
48	0.05364556038774418
49	0.020852450112068197
50	0.09097587758973485
51	0.1314367049469785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.459663E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.4204270051933
#Duplication Level	Relative count
1	100.0
2	11.763782514569012
3	3.8713348550947395
4	1.9575781755868482
5	1.294369264779944
6	0.8659650866946165
7	0.7031060938041637
8	0.5513659879785209
9	0.43036633462617646
10++	9.372567743454573
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50793	0.20650389911138234	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33711	0.13705536083601697	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13129140	5.8676686	7.5431147	18
AAAAA	6192525	3.6667833	3.9353468	47
TGTGT	5061035	3.589233	31.303028	4
TGTTT	4772030	2.6865752	25.047045	6
GTTTG	3746125	2.6567125	30.732166	7
GTGTT	3542175	2.5120733	30.793987	5
TTTGA	4111255	2.4485438	26.03405	8
CTTTG	3230485	2.4281723	32.84628	1
TTGTG	3314090	2.350318	30.75568	3
GAGGG	1842755	2.193818	9.268312	11
TTTGT	3710975	2.0892184	24.819674	2
TTGAG	2577115	1.9334536	18.993214	9
TGAGG	2028800	1.9173691	11.5638485	10
AGGGG	1374240	1.6360463	5.179037	12
ACTTT	2386620	1.5064898	12.077374	3
AACTT	2238585	1.494838	13.118069	2
TGAGT	1582360	1.1871492	5.38544	10
TTGAT	1874615	1.1164662	6.5887246	9
TAACT	1636365	1.0926993	12.869976	1
TTGAC	1191750	0.94762063	5.171499	9
>>END_MODULE