FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_1_CGATGT_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_1_CGATGT_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24596630
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64669 0.26291813146760346 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11793195 5.3876 6.79462 18
AAAAA 6397075 3.6365983 3.8919456 45
TGTGT 4186630 3.0960097 35.401077 4
TGTTT 4051745 2.3550105 28.018854 6
CTTTG 2940785 2.2472894 36.095776 1
GTTTG 3002400 2.2202725 34.79649 7
GAGGG 1658680 2.0742648 9.950304 11
TTTGA 3405415 2.067811 28.809135 8
GTGTT 2777850 2.054218 34.744408 5
TTTGT 3408675 1.9812367 27.736738 2
TTGTG 2588390 1.9141124 34.768333 3
TGAGG 1829445 1.7981837 12.792394 10
TTGAG 2146025 1.657916 20.670853 9
AGGGG 1231735 1.540348 5.52837 12
TGAGC 1394055 1.4159653 5.148533 10
TGAGT 1486790 1.1486228 5.782082 10
TTGAT 1722770 1.0460877 7.6157374 9
TTGAC 1111200 0.88711107 5.68812 9
Produced by FastQC (version 0.10.1)