##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_1_CGATGT_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	24596630	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75961845179604	34.0	31.0	34.0	31.0	34.0
2	33.000585202119154	34.0	31.0	34.0	31.0	34.0
3	33.08287119007767	34.0	33.0	34.0	31.0	34.0
4	36.491707685158495	37.0	37.0	37.0	35.0	37.0
5	36.41489655290176	37.0	37.0	37.0	35.0	37.0
6	36.39512595831218	37.0	37.0	37.0	35.0	37.0
7	36.40256457896874	37.0	37.0	37.0	35.0	37.0
8	36.38261790334692	37.0	37.0	37.0	35.0	37.0
9	38.19386761519769	39.0	39.0	39.0	37.0	39.0
10	38.19710098497233	39.0	39.0	39.0	37.0	39.0
11	38.185334860913876	39.0	39.0	39.0	37.0	39.0
12	38.153663652297084	39.0	39.0	39.0	37.0	39.0
13	38.134895999980486	39.0	38.0	39.0	37.0	39.0
14	39.69616878409766	41.0	40.0	41.0	38.0	41.0
15	39.69592549873702	41.0	40.0	41.0	38.0	41.0
16	39.669568961276404	41.0	40.0	41.0	37.0	41.0
17	39.654122495642696	41.0	40.0	41.0	37.0	41.0
18	39.63619357611185	41.0	40.0	41.0	37.0	41.0
19	39.642698085062875	41.0	40.0	41.0	37.0	41.0
20	39.61892909719746	41.0	40.0	41.0	37.0	41.0
21	39.59766687550287	41.0	40.0	41.0	37.0	41.0
22	39.56533825975347	41.0	40.0	41.0	37.0	41.0
23	39.531646815031166	41.0	40.0	41.0	37.0	41.0
24	39.49809010421346	41.0	39.0	41.0	37.0	41.0
25	39.445479766943684	41.0	39.0	41.0	37.0	41.0
26	39.373994242300675	41.0	39.0	41.0	36.0	41.0
27	39.29633766902214	41.0	39.0	41.0	36.0	41.0
28	39.2258641122788	40.0	39.0	41.0	36.0	41.0
29	39.16908031710035	40.0	39.0	41.0	36.0	41.0
30	39.1152384290043	40.0	39.0	41.0	36.0	41.0
31	39.0459873161486	40.0	39.0	41.0	35.0	41.0
32	38.9582867246448	40.0	39.0	41.0	35.0	41.0
33	38.898385144631604	40.0	38.0	41.0	35.0	41.0
34	39.04099927510395	40.0	39.0	41.0	35.0	41.0
35	39.066978321827015	40.0	39.0	41.0	35.0	41.0
36	39.04396041246301	40.0	39.0	41.0	35.0	41.0
37	39.01363032252792	40.0	39.0	41.0	35.0	41.0
38	38.97847058723085	40.0	39.0	41.0	35.0	41.0
39	38.91264091869496	40.0	39.0	41.0	35.0	41.0
40	38.73726006367539	40.0	38.0	41.0	35.0	41.0
41	38.75204363361973	40.0	38.0	41.0	35.0	41.0
42	38.587606228983404	40.0	38.0	41.0	35.0	41.0
43	38.5470436803741	40.0	38.0	41.0	35.0	41.0
44	38.575676830525154	40.0	38.0	41.0	35.0	41.0
45	38.56381170916504	40.0	38.0	41.0	35.0	41.0
46	38.49023675194529	40.0	38.0	41.0	35.0	41.0
47	38.43308526411951	40.0	38.0	41.0	35.0	41.0
48	38.38192711765799	40.0	38.0	41.0	34.0	41.0
49	38.32614967985452	40.0	38.0	41.0	34.0	41.0
50	38.412203582360675	40.0	38.0	41.0	35.0	41.0
51	38.12427222753686	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	2.0
9	2.0
10	11.0
11	20.0
12	50.0
13	101.0
14	198.0
15	318.0
16	520.0
17	846.0
18	1387.0
19	2340.0
20	4210.0
21	7111.0
22	11550.0
23	18022.0
24	26761.0
25	37487.0
26	50743.0
27	68019.0
28	89054.0
29	114952.0
30	148589.0
31	190491.0
32	247656.0
33	329520.0
34	475324.0
35	742277.0
36	1170992.0
37	1953995.0
38	4394750.0
39	1.4497375E7
40	11956.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.843181213843415	28.110018860866454	28.4882853487532	24.55851457653693
2	20.846542527380972	27.720644039611177	31.331016361925013	20.101797071082842
3	19.74689517461573	26.5951201011707	32.3578799560761	21.300104768137476
4	20.752241744279598	26.49827121418192	31.835719249851856	20.913767791686627
5	25.530672291285434	26.362054476568535	28.07979792353668	20.02747530860935
6	21.87108119786484	26.12460219406571	31.153227796009165	20.851088812060286
7	25.39873551783313	26.667482496585915	27.600915247332665	20.33286673824829
8	21.707412763455807	26.544083478102486	31.02949875653697	20.719005001904733
9	21.108525842767893	26.717721086181317	31.435822712298396	20.7379303587524
10	21.571755968195642	26.58860583746635	31.13582226508266	20.70381592925535
11	25.11920845706477	26.439914363840007	27.646482632430093	20.79439454666513
12	21.51787866874446	30.282937134070803	27.360642494520594	20.838541702664145
13	23.498328836104783	26.877031528302858	28.38261176429454	21.242027871297818
14	22.93846159600969	27.090089168485616	28.606845482872224	21.36460375263247
15	23.055483617064613	27.05103504016607	28.378916136072302	21.514565206697014
16	22.981993874770648	27.042497285197197	28.36033228942339	21.615176550608762
17	23.00059482933521	27.09933080683334	28.236873807952662	21.663200555878788
18	22.818991870024472	27.09075999435695	28.43670454041875	21.65354359519983
19	22.521231567088662	27.316319349439333	28.683079755234765	21.47936932823724
20	22.282609446903905	27.32007596162564	28.741160882608714	21.65615370886174
21	22.420071367500345	27.300618011491817	28.54582111451853	21.733489506489303
22	22.31460163445155	27.376888622547074	28.62563286108707	21.68287688191431
23	22.40339835172542	27.402538477832127	28.48399557175109	21.710067598691367
24	22.25848647297551	27.455811600734965	28.45618913237407	21.829512793915455
25	22.326302424356506	27.599427238609515	28.327356227255525	21.746914109778455
26	22.334401094784123	27.522937085283633	28.315094384881178	21.827567435051062
27	22.365592359603735	27.524628373886994	28.190390309566798	21.919388956942477
28	22.351992935617602	27.580957228693524	28.215881606545285	21.851168229143585
29	22.39894245675119	27.586401063885578	28.160565085542206	21.854091393821022
30	22.40080043485632	27.51944880253921	28.141672253475374	21.938078509129095
31	22.49998881960659	27.478658661776024	28.05854297926179	21.962809539355593
32	22.54297490001169	27.544413105456538	27.970024342770607	21.942587651761166
33	22.46793564809488	27.465998390836464	27.95012162235233	22.11594433871632
34	22.4616658462282	27.537992643212046	27.92795298082613	22.072388529733615
35	22.527449920944388	27.51558039908752	27.885184177819628	22.071785502148465
36	22.5729459686144	27.499242782446213	27.85005506851955	22.07775618041984
37	22.59831529766476	27.470238809137676	27.86263402750702	22.068811865690545
38	22.560822356558603	27.498157267885887	27.901578386957887	22.039441988597623
39	22.502029749603906	27.436949695954283	27.904070598289277	22.15694995615253
40	22.61003641555774	27.505267998095672	27.84264348408705	22.04205210225954
41	22.560502574372162	27.426329245172738	27.89992426822264	22.113243912232463
42	22.58979031204479	27.51113257938599	27.762639897439072	22.136437211130154
43	22.551233047266976	27.574446802130243	27.741380280480772	22.132939870122016
44	22.59247002230388	27.40949366213732	27.812890551628637	22.185145763930166
45	22.598624168015363	27.37171727093427	27.776882893771372	22.252775667279
46	22.52351855228788	27.513962726893677	27.752292188496458	22.210226532321986
47	22.55232655843412	27.513915819260426	27.733211166907978	22.200546455397472
48	22.505465114647972	27.41069342968497	27.734930809097186	22.348910646569873
49	22.58020306033794	27.456179972622262	27.729969512083564	22.23364745495623
50	22.522854674208652	27.410256521805778	27.735798433334637	22.33109037065094
51	22.572417250359702	27.40448672346681	27.701036933056024	22.322059093117467
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.401695790380344
2	40.94833959846381
3	41.0469999427532
4	41.666009535966225
5	45.55814759989478
6	42.72217000992512
7	45.73160225608142
8	42.42641776536054
9	41.84645620152029
10	42.275571897450995
11	45.913603003729904
12	42.3564203714086
13	44.740356707402604
14	44.303065348642164
15	44.570048823761624
16	44.59717042537941
17	44.663795385214
18	44.4725354652243
19	44.0006008953259
20	43.93876315576565
21	44.15356087398965
22	43.99747851636586
23	44.11346595041679
24	44.08799926689097
25	44.073216534134964
26	44.161968529835185
27	44.284981316546215
28	44.20316116476119
29	44.25303385057221
30	44.338878943985414
31	44.46279835896219
32	44.48556255177286
33	44.583879986811205
34	44.534054375961816
35	44.59923542309286
36	44.65070214903424
37	44.6671271633553
38	44.600264345156226
39	44.658979705756444
40	44.652088517817276
41	44.67374648660463
42	44.72622752317494
43	44.684172917388985
44	44.777615786234044
45	44.851399835294366
46	44.733745084609865
47	44.75287301383159
48	44.85437576121784
49	44.81385051529417
50	44.85394504485959
51	44.894476343477166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43211.0
1	25374.5
2	7538.0
3	10337.5
4	13137.0
5	26476.5
6	39816.0
7	69615.0
8	99414.0
9	60314.0
10	21214.0
11	24269.0
12	27324.0
13	32702.5
14	38081.0
15	52819.5
16	67558.0
17	96182.5
18	124807.0
19	155720.0
20	186633.0
21	219426.5
22	252220.0
23	301329.0
24	350438.0
25	414608.0
26	557960.0
27	637142.0
28	691205.5
29	745269.0
30	796909.0
31	848549.0
32	917540.0
33	986531.0
34	1048611.5
35	1110692.0
36	1214084.5
37	1317477.0
38	1377797.5
39	1438118.0
40	1504178.5
41	1570239.0
42	1595602.0
43	1620965.0
44	1636876.5
45	1652788.0
46	1641011.5
47	1629235.0
48	1608030.0
49	1586825.0
50	1530691.0
51	1474557.0
52	1390569.0
53	1306581.0
54	1213797.0
55	1121013.0
56	1026725.0
57	932437.0
58	834709.5
59	736982.0
60	655022.5
61	573063.0
62	492853.0
63	412643.0
64	356141.5
65	299640.0
66	249569.0
67	199498.0
68	183324.0
69	167150.0
70	147012.0
71	126874.0
72	107372.0
73	87870.0
74	73707.5
75	49555.0
76	39565.0
77	37222.5
78	34880.0
79	35261.0
80	35642.0
81	31237.0
82	26832.0
83	23614.5
84	20397.0
85	18342.0
86	16287.0
87	19709.0
88	23131.0
89	14775.5
90	6420.0
91	3767.5
92	1115.0
93	752.5
94	390.0
95	240.0
96	90.0
97	55.5
98	21.0
99	14.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022364852420839763
2	0.0017929285434630678
3	0.005553606327370863
4	0.030113881454491936
5	0.0
6	0.001894568483568684
7	0.0
8	0.0
9	0.0
10	0.0
11	4.472157364647108E-5
12	0.0
13	0.0
14	0.007488830786981794
15	0.0
16	0.0
17	0.005447900789661023
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	6.50495616675943E-5
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	2.032798802112322E-5
33	0.0
34	1.0570553770984074E-4
35	8.131195208449288E-5
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.013233520201751215
42	5.24462090944979E-4
43	2.4393585625347862E-5
44	4.065597604224644E-5
45	8.944314729294216E-5
46	0.006850531963118524
47	0.011615412355269807
48	0.008110867220428164
49	0.0
50	0.00541944160643145
51	0.06766780652471496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.459663E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.729684984843136
#Duplication Level	Relative count
1	100.0
2	12.318101770321624
3	4.114640573070456
4	2.1655654568806852
5	1.3227758431206387
6	0.9977093070984998
7	0.6984627199661031
8	0.5581081260013506
9	0.4753518795598692
10++	9.75927863035102
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64669	0.26291813146760346	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11793195	5.3876	6.79462	18
AAAAA	6397075	3.6365983	3.8919456	45
TGTGT	4186630	3.0960097	35.401077	4
TGTTT	4051745	2.3550105	28.018854	6
CTTTG	2940785	2.2472894	36.095776	1
GTTTG	3002400	2.2202725	34.79649	7
GAGGG	1658680	2.0742648	9.950304	11
TTTGA	3405415	2.067811	28.809135	8
GTGTT	2777850	2.054218	34.744408	5
TTTGT	3408675	1.9812367	27.736738	2
TTGTG	2588390	1.9141124	34.768333	3
TGAGG	1829445	1.7981837	12.792394	10
TTGAG	2146025	1.657916	20.670853	9
AGGGG	1231735	1.540348	5.52837	12
TGAGC	1394055	1.4159653	5.148533	10
TGAGT	1486790	1.1486228	5.782082	10
TTGAT	1722770	1.0460877	7.6157374	9
TTGAC	1111200	0.88711107	5.68812	9
>>END_MODULE