FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_18_CCGTCC_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_18_CCGTCC_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 37169300
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11129890 3.417264 4.143156 16
TGTGT 5892200 2.9376292 21.135567 4
TGTTT 5920630 2.3164425 16.813358 6
GTTTG 4444695 2.2159576 20.622324 7
CTTTG 3939555 2.0394864 21.62692 1
GAGGG 2438375 2.0271158 6.517264 11
TTTGA 5031860 2.0216637 16.86304 8
GTGTT 3881335 1.9350877 20.496765 5
TTGTG 3653505 1.8215001 20.535734 3
TTTGT 4633025 1.8126677 16.526632 2
TGAGG 2580250 1.6833482 7.874905 10
TTGAG 3134470 1.6047612 12.586776 9
ACTTT 3321850 1.3858445 6.3777256 3
AACTT 3194325 1.368486 6.733674 2
TAACT 2266585 0.9710314 6.403194 1
Produced by FastQC (version 0.10.1)