##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_18_CCGTCC_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	37169300	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.272520601679346	34.0	31.0	34.0	31.0	34.0
2	32.42399509810516	34.0	31.0	34.0	31.0	34.0
3	32.47913842875707	34.0	31.0	34.0	31.0	34.0
4	35.9230641685477	37.0	37.0	37.0	35.0	37.0
5	35.93232778126034	37.0	37.0	37.0	35.0	37.0
6	35.92712224873753	37.0	37.0	37.0	35.0	37.0
7	35.943835907590405	37.0	37.0	37.0	35.0	37.0
8	35.901191171208495	37.0	37.0	37.0	35.0	37.0
9	37.66850667082781	39.0	38.0	39.0	35.0	39.0
10	37.604629062156135	39.0	38.0	39.0	35.0	39.0
11	37.61490706039662	39.0	38.0	39.0	35.0	39.0
12	37.59239953402405	39.0	38.0	39.0	35.0	39.0
13	37.5786077219641	39.0	38.0	39.0	35.0	39.0
14	39.04019508034857	41.0	39.0	41.0	36.0	41.0
15	39.01609198989489	41.0	39.0	41.0	36.0	41.0
16	38.998187832431604	41.0	39.0	41.0	36.0	41.0
17	38.973002074292495	41.0	39.0	41.0	36.0	41.0
18	38.91224074706815	41.0	39.0	41.0	36.0	41.0
19	38.926457990868805	41.0	39.0	41.0	36.0	41.0
20	38.910938032193236	41.0	39.0	41.0	36.0	41.0
21	38.875322672205286	41.0	39.0	41.0	35.0	41.0
22	38.82625626525116	41.0	39.0	41.0	35.0	41.0
23	38.76766557347058	40.0	39.0	41.0	35.0	41.0
24	38.7243577898965	41.0	39.0	41.0	35.0	41.0
25	38.660626646183815	40.0	39.0	41.0	35.0	41.0
26	38.609754232659746	40.0	39.0	41.0	35.0	41.0
27	38.575653025480705	40.0	39.0	41.0	34.0	41.0
28	38.53439125837721	40.0	38.0	41.0	34.0	41.0
29	38.41825856284622	40.0	38.0	41.0	34.0	41.0
30	38.367246598671485	40.0	38.0	41.0	34.0	41.0
31	38.331429405450194	40.0	38.0	41.0	34.0	41.0
32	38.28143868730377	40.0	38.0	41.0	34.0	41.0
33	38.21610202505831	40.0	38.0	41.0	34.0	41.0
34	38.16026645645734	40.0	38.0	41.0	34.0	41.0
35	38.10323756433401	40.0	38.0	41.0	33.0	41.0
36	38.049387505279896	40.0	38.0	41.0	33.0	41.0
37	37.98767356393583	40.0	38.0	41.0	33.0	41.0
38	37.90823671686042	40.0	38.0	41.0	33.0	41.0
39	37.845185892658726	40.0	38.0	41.0	33.0	41.0
40	37.766057391449394	40.0	38.0	41.0	33.0	41.0
41	37.95120892779794	40.0	38.0	41.0	33.0	41.0
42	37.94157420236593	40.0	38.0	41.0	33.0	41.0
43	37.94106819337464	40.0	38.0	41.0	33.0	41.0
44	37.88082842560931	40.0	38.0	41.0	33.0	41.0
45	37.85419149674597	40.0	38.0	41.0	33.0	41.0
46	37.79956967174523	40.0	38.0	41.0	33.0	41.0
47	37.75360450694525	40.0	38.0	41.0	33.0	41.0
48	37.6721343420511	40.0	37.0	41.0	33.0	41.0
49	37.591638691070315	40.0	37.0	41.0	32.0	41.0
50	37.515943560949495	40.0	37.0	41.0	32.0	41.0
51	35.998900086899674	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	30.0
5	78.0
6	867.0
7	3761.0
8	12031.0
9	19155.0
10	22386.0
11	26400.0
12	33609.0
13	42649.0
14	50855.0
15	56525.0
16	59901.0
17	61449.0
18	62816.0
19	64680.0
20	68097.0
21	73062.0
22	79461.0
23	88138.0
24	100445.0
25	115604.0
26	134549.0
27	158563.0
28	190587.0
29	230373.0
30	281447.0
31	351351.0
32	456240.0
33	611745.0
34	872182.0
35	1290811.0
36	1977948.0
37	3256159.0
38	7259050.0
39	1.9052859E7
40	3433.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.399875897884563	28.323885051081476	28.920799921889333	23.355439129144628
2	22.385602965557027	29.231742094987222	29.75414004708652	18.62851489236923
3	21.789687298800438	27.749503063728508	29.936060323838092	20.524749313632963
4	21.49596187715002	26.534871419862	30.56533181806197	21.40383488492601
5	24.46903800200937	26.797422565847583	28.742387150893318	19.991152281249732
6	21.869668533537805	26.589231194784162	30.812838919870977	20.728261351807056
7	23.796868361540994	27.166335543179404	28.86144309274109	20.17535300253851
8	21.802861436592625	26.99272930943723	30.59292065019695	20.611488603773196
9	21.90755363805625	26.81088721400694	30.67171328709432	20.609845860842487
10	21.9436537148135	27.777299924627147	29.72896578841981	20.550080572139542
11	23.829503606753615	26.916327502904252	28.51427948098567	20.739889409356458
12	21.566185213067598	29.088185489053252	28.51012525055601	20.835504047323145
13	22.88962693146796	27.215692170895732	28.863948298428454	21.030732599207855
14	23.0413213063222	27.456097946640735	28.37339525436567	21.1291854926714
15	22.55465606466756	27.94225345090664	28.192040714156203	21.3110497702696
16	22.73797911065642	27.46974033709874	28.364956010167457	21.42732454207738
17	22.642406549128555	27.51400030942883	28.44486987172157	21.398723269721042
18	22.569061821418185	27.47973755202993	28.399308984944515	21.55189164160737
19	22.328852846661356	27.646661430881732	28.59577685135919	21.428708871097722
20	22.21765189909706	27.684715290617802	28.54889648893446	21.548736321350685
21	22.283069919111114	27.68758929125615	28.448386240427453	21.580954549205288
22	22.192730941565983	27.750183691469804	28.54810865062496	21.50897671633925
23	22.252113479471305	27.841160982266793	28.392475495981817	21.514250042280082
24	22.146251191241927	27.816547949235815	28.38397716240109	21.653223697121167
25	22.162873210698883	27.962780297874296	28.280610077317203	21.593736414109618
26	22.210349465530825	27.918077889117583	28.239745774743124	21.631826870608467
27	22.18440028664819	27.854440435700944	28.193682971622152	21.767476306028712
28	22.185947020236423	27.891278080188226	28.19599426156382	21.72678063801153
29	22.21019822908307	27.93479442984717	28.160049487380185	21.694957853689573
30	22.24434956980027	27.925381709980496	28.068298438166433	21.761970282052797
31	22.307444882658174	27.878532611028323	28.029552961088438	21.784469545225065
32	22.348042146325373	27.904339310742625	27.942873666039286	21.80474487689272
33	22.300068746784095	27.84466493659174	27.943001873657312	21.91226444296685
34	22.339584876895124	27.878846077814707	27.90792642534814	21.873642619942036
35	22.343803728700866	27.864995640213202	27.868130725466262	21.92306990561967
36	22.380146137191577	27.858724719702792	27.852315004921653	21.90881413818398
37	22.346030856436748	27.832276235747116	27.87740568986883	21.944287217947302
38	22.354268746991085	27.868549498815444	27.940110560885103	21.837071193308375
39	22.334613809919528	27.767797940923888	27.918138746431293	21.97944950272529
40	22.36378897089068	27.761447110280322	27.892839606936963	21.98192431189203
41	22.351571707647846	27.77870162579312	27.9144561584785	21.95527050808053
42	22.34285443633093	27.774156688337587	27.8710407154579	22.011948159873583
43	22.34509213000348	27.819540353158175	27.83848698246804	21.9968805343703
44	22.44615660262437	27.783580768923517	27.78758446060708	21.982678167845034
45	22.40939881037092	27.74631290125545	27.8064817065517	22.037806581821933
46	22.334711904945955	27.818996640741023	27.77121908953122	22.075072364781807
47	22.37212636094351	27.816368754957853	27.77429552643767	22.037209357660963
48	22.36221068512834	27.727329052809534	27.756330769464565	22.154129492597562
49	22.411245238673644	27.879950587364345	27.690202536051757	22.01860163791026
50	22.338096023609204	27.819787860383073	27.780216264238323	22.061899851769397
51	22.380465247784333	27.749384423556243	27.769827315345385	22.100323013314036
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.75531502702919
2	41.01411785792626
3	42.3144366124334
4	42.89979676207603
5	44.4601902832591
6	42.59792988534486
7	43.97222136407951
8	42.41435004036582
9	42.51739949889874
10	42.49373428695304
11	44.56939301611008
12	42.40168926039074
13	43.92035953067581
14	44.1705067989936
15	43.86570583493716
16	44.1653036527338
17	44.0411298188496
18	44.12095346302556
19	43.757561717759074
20	43.766388220447745
21	43.8640244683164
22	43.70170765790524
23	43.76636352175139
24	43.79947488836309
25	43.756609624808505
26	43.842176336139296
27	43.9518765926769
28	43.91272765824795
29	43.90515608277264
30	44.00631985185307
31	44.091914427883246
32	44.152787023218096
33	44.21233318975094
34	44.21322749683716
35	44.26687363432053
36	44.28896027537556
37	44.29031807438405
38	44.19133994029946
39	44.31406331264482
40	44.34571328278271
41	44.30684221572837
42	44.354802596204514
43	44.341972664373785
44	44.42883477046941
45	44.44720539219285
46	44.40978426972776
47	44.40933571860447
48	44.5163401777259
49	44.4298468765839
50	44.3999958753786
51	44.48078826109837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14804.0
1	10098.5
2	5393.0
3	7613.0
4	9833.0
5	19440.5
6	29048.0
7	35528.5
8	42009.0
9	34542.0
10	27075.0
11	30306.5
12	33538.0
13	42984.5
14	52431.0
15	75852.5
16	99274.0
17	140095.0
18	180916.0
19	231920.5
20	282925.0
21	344108.0
22	405291.0
23	478156.5
24	551022.0
25	654914.0
26	899414.5
27	1040023.0
28	1130223.0
29	1220423.0
30	1302601.0
31	1384779.0
32	1492786.0
33	1600793.0
34	1701066.0
35	1801339.0
36	1974151.5
37	2146964.0
38	2238063.5
39	2329163.0
40	2437838.0
41	2546513.0
42	2547431.5
43	2548350.0
44	2549335.0
45	2550320.0
46	2500154.0
47	2449988.0
48	2384602.5
49	2319217.0
50	2211652.0
51	2104087.0
52	1958359.5
53	1812632.0
54	1676504.5
55	1540377.0
56	1404590.5
57	1268804.0
58	1125221.0
59	981638.0
60	873583.0
61	765528.0
62	661305.0
63	557082.0
64	478667.0
65	400252.0
66	337100.0
67	273948.0
68	258026.0
69	242104.0
70	218938.5
71	195773.0
72	167380.0
73	138987.0
74	118928.5
75	83377.5
76	67885.0
77	63549.0
78	59213.0
79	58786.0
80	58359.0
81	51601.5
82	44844.0
83	40562.5
84	36281.0
85	33167.5
86	30054.0
87	34947.5
88	39841.0
89	26870.0
90	13899.0
91	9009.5
92	4120.0
93	3261.0
94	2402.0
95	1827.0
96	1252.0
97	951.5
98	651.0
99	415.5
100	180.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004092086749010609
2	0.0018859650302803658
3	1.264484399760017E-4
4	0.0027980080335115267
5	0.0010680857589462271
6	0.0037853820222603063
7	0.024062869088199133
8	0.011278124688923386
9	0.013868972512261463
10	0.0020043422932366226
11	0.01305916441794707
12	0.02138323831764386
13	0.01244844535678638
14	0.005275859378573177
15	0.006653340256609621
16	0.037385691955457864
17	0.010896088976655465
18	0.0021254099485327944
19	0.0293548708208118
20	0.0053242864406916465
21	0.0024401858523028412
22	0.024654755402980413
23	0.001339815385277635
24	0.07086762462569916
25	0.02040393550591483
26	0.0357149583123707
27	0.06112571396286721
28	0.01577915107360106
29	0.10716908846817132
30	0.05298727713462455
31	0.021746441283532376
32	0.03456077999854718
33	0.022300662105554852
34	0.03665121484666109
35	0.024821559728055144
36	0.01872513068580791
37	0.025647510176409025
38	0.07423599583527266
39	0.03869860341733635
40	0.056659662678608426
41	0.015873314805498084
42	0.028087696028711865
43	0.03307837381925406
44	0.009688102816033661
45	0.032747455561444525
46	0.042691145649770106
47	0.04683704024557901
48	0.06247898130984441
49	0.017046325865700995
50	0.0712873258307259
51	0.12475887358653513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.71693E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.488511555825074
#Duplication Level	Relative count
1	100.0
2	11.59276994759052
3	3.373335142785134
4	1.5425576601203286
5	0.9648255494594262
6	0.6643273039814402
7	0.48144342555075187
8	0.3877396715844982
9	0.3056681077657794
10++	9.933890385994843
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11129890	3.417264	4.143156	16
TGTGT	5892200	2.9376292	21.135567	4
TGTTT	5920630	2.3164425	16.813358	6
GTTTG	4444695	2.2159576	20.622324	7
CTTTG	3939555	2.0394864	21.62692	1
GAGGG	2438375	2.0271158	6.517264	11
TTTGA	5031860	2.0216637	16.86304	8
GTGTT	3881335	1.9350877	20.496765	5
TTGTG	3653505	1.8215001	20.535734	3
TTTGT	4633025	1.8126677	16.526632	2
TGAGG	2580250	1.6833482	7.874905	10
TTGAG	3134470	1.6047612	12.586776	9
ACTTT	3321850	1.3858445	6.3777256	3
AACTT	3194325	1.368486	6.733674	2
TAACT	2266585	0.9710314	6.403194	1
>>END_MODULE