##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_18_CCGTCC_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	37169300	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.728562361949244	34.0	31.0	34.0	31.0	34.0
2	32.954079764752095	34.0	31.0	34.0	31.0	34.0
3	33.03034603826276	34.0	31.0	34.0	31.0	34.0
4	36.423653714221146	37.0	37.0	37.0	35.0	37.0
5	36.35317226851192	37.0	37.0	37.0	35.0	37.0
6	36.33185168943187	37.0	37.0	37.0	35.0	37.0
7	36.31477808298784	37.0	37.0	37.0	35.0	37.0
8	36.30565375188664	37.0	37.0	37.0	35.0	37.0
9	38.05950407997998	39.0	38.0	39.0	37.0	39.0
10	38.043908117720804	39.0	38.0	39.0	37.0	39.0
11	38.008906220994206	39.0	38.0	39.0	35.0	39.0
12	37.990712927066156	39.0	38.0	39.0	35.0	39.0
13	37.991201825162165	39.0	38.0	39.0	35.0	39.0
14	39.52733920197583	41.0	40.0	41.0	37.0	41.0
15	39.52871818947357	41.0	39.0	41.0	37.0	41.0
16	39.524598553107	41.0	39.0	41.0	37.0	41.0
17	39.499351588542154	41.0	39.0	41.0	37.0	41.0
18	39.45516450403962	41.0	39.0	41.0	37.0	41.0
19	39.4894508640195	41.0	39.0	41.0	37.0	41.0
20	39.46101605357109	41.0	39.0	41.0	37.0	41.0
21	39.42372514413777	41.0	39.0	41.0	37.0	41.0
22	39.368212449521515	41.0	39.0	41.0	36.0	41.0
23	39.329898975767634	41.0	39.0	41.0	36.0	41.0
24	39.27934179551404	41.0	39.0	41.0	36.0	41.0
25	39.24626858724808	41.0	39.0	41.0	36.0	41.0
26	39.18348042605053	40.0	39.0	41.0	36.0	41.0
27	39.12673574697398	40.0	39.0	41.0	36.0	41.0
28	39.058111640520536	40.0	39.0	41.0	36.0	41.0
29	38.981298975229556	40.0	39.0	41.0	36.0	41.0
30	38.93229775648183	40.0	39.0	41.0	35.0	41.0
31	38.85116125404568	40.0	38.0	41.0	35.0	41.0
32	38.797205866131456	40.0	38.0	41.0	35.0	41.0
33	38.72094529625255	40.0	38.0	41.0	35.0	41.0
34	38.82362395848187	40.0	38.0	41.0	35.0	41.0
35	38.827123217278775	40.0	38.0	41.0	35.0	41.0
36	38.8248657628742	40.0	38.0	41.0	35.0	41.0
37	38.770034141078796	40.0	38.0	41.0	35.0	41.0
38	38.72560223087333	40.0	38.0	41.0	35.0	41.0
39	38.6230817637136	40.0	38.0	41.0	35.0	41.0
40	38.60653759957815	40.0	38.0	41.0	35.0	41.0
41	38.573436007672996	40.0	38.0	41.0	35.0	41.0
42	38.50807534712787	40.0	38.0	41.0	35.0	41.0
43	38.434701110862996	40.0	38.0	41.0	34.0	41.0
44	38.31537556531869	40.0	38.0	41.0	34.0	41.0
45	38.28694627017458	40.0	38.0	41.0	34.0	41.0
46	38.20288149090782	40.0	38.0	41.0	34.0	41.0
47	38.18354195532334	40.0	38.0	41.0	34.0	41.0
48	38.111512888324505	40.0	38.0	41.0	34.0	41.0
49	38.068029556650245	40.0	38.0	41.0	33.0	41.0
50	38.141624162951686	40.0	38.0	41.0	34.0	41.0
51	37.86855324151921	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	14.0
11	35.0
12	65.0
13	71.0
14	146.0
15	254.0
16	468.0
17	791.0
18	1535.0
19	3020.0
20	5573.0
21	10438.0
22	17871.0
23	28823.0
24	43240.0
25	63094.0
26	87430.0
27	118460.0
28	158076.0
29	207833.0
30	270829.0
31	349658.0
32	454040.0
33	604344.0
34	856067.0
35	1274334.0
36	1954312.0
37	3241998.0
38	7148718.0
39	2.025623E7
40	11524.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.847994929569932	28.873125065611955	28.46835934143505	23.810520663383063
2	21.47180616117856	28.52708673418256	30.69643876857141	19.304668336067465
3	19.855473481683667	27.282610190009	31.767154076108795	21.094762252198542
4	20.664564256214554	27.18804508256224	31.27567030648214	20.87172035474106
5	23.970794941048457	27.294588653206592	28.771422849224017	19.963193556520935
6	22.108504184143477	27.107498091329337	30.028783213674338	20.75521451085285
7	23.876061696077137	27.83679272948374	27.96442763248164	20.322717941957475
8	21.401597554971442	27.307743218193508	30.38837965740544	20.90227956942961
9	21.112810841204972	27.244962912941595	30.796192018682085	20.846034227171348
10	21.47432044619611	27.433886479649626	30.284685485314245	20.80710758884002
11	23.533597128468863	27.37243568803055	28.230390471649535	20.86357671185105
12	21.43392261893552	29.54612812186401	28.056686566602007	20.963262692598462
13	22.665522353124757	27.68148445087747	28.528578692630745	21.124414503367024
14	22.346172782376854	27.7793286394955	28.643714032817407	21.230784545310243
15	22.345753081171825	27.72428321222084	28.529786678791368	21.400177027815968
16	22.306879602252398	27.692103429443115	28.531774878730566	21.46924208957392
17	22.355814197282516	27.709557488444318	28.460566356929455	21.474061957343704
18	22.270559305663546	27.6690575286594	28.487905341235916	21.572477824441137
19	22.040834774935227	27.833787561240054	28.652199530257498	21.47317813356722
20	21.976798056460574	27.865031087483487	28.629099821626987	21.529071034428952
21	22.069601563441637	27.798561069786665	28.49649764536736	21.635339721404343
22	22.03887347891943	27.849703922322995	28.561788895674656	21.54963370308292
23	22.13089834890622	27.883384944026385	28.41009381398089	21.5756228930865
24	22.020382412367194	27.894937488733984	28.387599443626865	21.697080655271957
25	22.060485400585968	27.986800935180376	28.33376738329751	21.61894628093615
26	22.112428805492705	27.94468015270667	28.265506748849184	21.67738429295144
27	22.08829598620367	27.89547018641729	28.200391183046225	21.81584264433282
28	22.11416948933663	27.953383571926295	28.212352667389485	21.72009427134759
29	22.14604794817228	27.997597479640458	28.183022010099734	21.67333256208753
30	22.142327135566177	27.912481537182565	28.176150748063588	21.769040579187664
31	22.236821785721013	27.875464428977676	28.08316809840379	21.80454568689752
32	22.26027393574805	27.934074626102724	28.01827045438036	21.787380983768863
33	22.233981672816434	27.875497532779796	28.007609749792834	21.882911044610935
34	22.221723596013657	27.915642991992932	27.986315185769694	21.876318226223717
35	22.243198233965032	27.888345833519534	27.959620449486916	21.90883548302852
36	22.30535276025052	27.909245310902538	27.905260834808814	21.880141094038127
37	22.285741374327976	27.899820433776874	27.928726229160162	21.885711962734984
38	22.28788274194026	27.915758435052584	27.971237015493973	21.825121807513188
39	22.302869176144295	27.82884982747579	27.927119161535458	21.941161834844454
40	22.28744906812232	27.839890042458748	27.963193559752714	21.909467329666217
41	22.28356842500995	27.796043147047556	27.994023299573595	21.9263651283689
42	22.264435260661223	27.825529300336154	27.933034138578634	21.97700130042399
43	22.276833047214353	27.878654272162308	27.8916623299748	21.952850350648543
44	22.419398722488477	27.825778122817674	27.827249767704753	21.927573386989092
45	22.366038306520228	27.760059793790415	27.866417021870088	22.00748487781927
46	22.27391651084214	27.87957721480722	27.855428023167512	21.991078251183126
47	22.29946776729124	27.859817816188976	27.8804687875434	21.960245628976388
48	22.32667097853432	27.78340325925644	27.833103009216003	22.056822752993238
49	22.400725867853307	27.848213444966678	27.804817416523854	21.94624327065616
50	22.281045789093564	27.849597259387203	27.828751953860596	22.040604997658637
51	22.325173576376354	27.77169229303974	27.82541658276455	22.077717547819358
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.65851559295299
2	40.77647449724603
3	40.95023573388221
4	41.53628461095561
5	43.933988497569395
6	42.86371869499632
7	44.198779638034615
8	42.303877124401055
9	41.95884506837632
10	42.28142803503613
11	44.397173840319915
12	42.39718531153398
13	43.789936856491785
14	43.5769573276871
15	43.74593010898779
16	43.77612169182632
17	43.82987615462623
18	43.84303713010468
19	43.514012908502444
20	43.505869090889526
21	43.70494128484598
22	43.58850718200235
23	43.70652124199272
24	43.71746306763916
25	43.67943168152212
26	43.78981309844415
27	43.904138630536494
28	43.83426376068422
29	43.81938051025981
30	43.911367714753844
31	44.04136747261853
32	44.047654919516916
33	44.11689271742737
34	44.09804182223737
35	44.15203371699356
36	44.18549385428865
37	44.17145333706296
38	44.11300454945345
39	44.244031010988756
40	44.19691639778854
41	44.209933553378846
42	44.24143656108521
43	44.22968339786289
44	44.34697210947757
45	44.3735231843395
46	44.26499476202527
47	44.25971339626763
48	44.38349373152756
49	44.346969138509465
50	44.3216507867522
51	44.40289112419571
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22391.0
1	14097.0
2	5803.0
3	8568.0
4	11333.0
5	14449.0
6	17565.0
7	27813.5
8	38062.0
9	31523.0
10	24984.0
11	28475.5
12	31967.0
13	42483.0
14	52999.0
15	78308.0
16	103617.0
17	147110.0
18	190603.0
19	245364.0
20	300125.0
21	362655.5
22	425186.0
23	500877.0
24	576568.0
25	685869.5
26	941775.0
27	1088379.0
28	1174822.5
29	1261266.0
30	1336654.0
31	1412042.0
32	1516843.5
33	1621645.0
34	1719117.0
35	1816589.0
36	1982627.5
37	2148666.0
38	2234043.5
39	2319421.0
40	2420023.5
41	2520626.0
42	2519830.0
43	2519034.0
44	2518854.0
45	2518674.0
46	2466392.5
47	2414111.0
48	2354786.5
49	2295462.0
50	2187134.0
51	2078806.0
52	1939424.5
53	1800043.0
54	1665272.5
55	1530502.0
56	1400028.5
57	1269555.0
58	1123159.0
59	976763.0
60	869217.5
61	761672.0
62	656629.5
63	551587.0
64	473253.0
65	394919.0
66	331885.5
67	268852.0
68	254553.0
69	240254.0
70	217243.0
71	194232.0
72	166064.0
73	137896.0
74	117295.5
75	80544.5
76	64394.0
77	61385.5
78	58377.0
79	58076.5
80	57776.0
81	50963.0
82	44150.0
83	38903.5
84	33657.0
85	30978.0
86	28299.0
87	32927.5
88	37556.0
89	23364.0
90	9172.0
91	5172.5
92	1173.0
93	864.5
94	556.0
95	320.0
96	84.0
97	57.5
98	31.0
99	20.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025647510176409025
2	7.398578934766058E-4
3	0.0012940787154990814
4	0.027885916603218246
5	0.0021738370106512634
6	0.0020473885706752616
7	0.0
8	0.0
9	0.0
10	9.470181036500553E-4
11	0.0015012389256725308
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.013161399326863835
18	0.0
19	0.0
20	0.0
21	5.38078467982986E-5
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	3.793453199280051E-4
34	0.0013694097010166992
35	0.0010411818355470779
36	1.264484399760017E-4
37	7.613810321959252E-4
38	0.0
39	6.456941615795831E-5
40	1.1837726295625692E-4
41	0.018803152063665445
42	0.02451485500130484
43	8.340216253736283E-5
44	1.8832746379404508E-5
45	5.999574918010293E-4
46	9.523988883298853E-4
47	0.01070238072818159
48	0.005679418229560417
49	0.0
50	0.0136053140629498
51	0.11489051448372717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.71693E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.765108973275073
#Duplication Level	Relative count
1	100.0
2	12.173697561739063
3	3.556639516912623
4	1.6342021689371165
5	0.9468721266148017
6	0.6723313791596836
7	0.4929995369977893
8	0.4030075580220024
9	0.3475777158992351
10++	10.195830371640788
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	10685675	3.240356	3.933143	16
TGTGT	5262000	2.6576953	21.935019	4
TGTTT	5318605	2.0814707	17.17894	6
GAGGG	2255480	1.9451905	6.7086973	11
GTTTG	3824455	1.9316301	21.308067	7
CTTTG	3707890	1.920323	21.993174	1
TTTGA	4462485	1.7904183	17.212732	8
TTTGT	4402130	1.7228022	16.89487	2
GTGTT	3249870	1.6414223	21.197857	5
TGAGG	2422425	1.6187943	8.253364	10
TTGTG	3099945	1.5656995	21.220146	3
TTGAG	2789560	1.444426	13.009384	9
>>END_MODULE