FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_16_GGCTAC_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_16_GGCTAC_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 27772345
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8285185 3.4813685 4.2439685 16
TGTGT 4973525 3.2862427 25.161476 4
AAAAA 6294970 3.0875256 3.3016446 10
TGTTT 4727645 2.491073 20.286818 6
GTTTG 3529050 2.33181 24.549187 7
TTTGA 4001505 2.174697 20.456282 8
CTTTG 3129930 2.161163 25.83627 1
GTGTT 3214860 2.1242096 24.479673 5
GAGGG 1911480 2.0484548 7.4131017 11
TTGTG 3009045 1.9882181 24.485538 3
TTTGT 3709045 1.9543557 19.948524 2
TGAGG 2041005 1.7442427 9.265737 10
TTGAG 2545265 1.734611 15.281871 9
ACTTT 2482630 1.4099541 7.7070327 3
AACTT 2378590 1.3933058 8.212939 2
TAACT 1716080 1.0052274 7.9528813 1
Produced by FastQC (version 0.10.1)