##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_16_GGCTAC_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	27772345	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55248345071329	34.0	31.0	34.0	31.0	34.0
2	32.71939139456895	34.0	31.0	34.0	31.0	34.0
3	32.75800689498852	34.0	31.0	34.0	31.0	34.0
4	36.15614774337565	37.0	37.0	37.0	35.0	37.0
5	36.16273786027071	37.0	37.0	37.0	35.0	37.0
6	36.18883281192136	37.0	37.0	37.0	35.0	37.0
7	36.19575696614744	37.0	37.0	37.0	35.0	37.0
8	36.1914814899498	37.0	37.0	37.0	35.0	37.0
9	37.96296456060876	39.0	39.0	39.0	37.0	39.0
10	37.94512206297308	39.0	39.0	39.0	37.0	39.0
11	37.945542229149176	39.0	39.0	39.0	37.0	39.0
12	37.88939248738268	39.0	39.0	39.0	37.0	39.0
13	37.890482024474345	39.0	39.0	39.0	37.0	39.0
14	39.44380613160322	41.0	40.0	41.0	37.0	41.0
15	39.39512558266146	41.0	40.0	41.0	37.0	41.0
16	39.364799299446986	41.0	40.0	41.0	37.0	41.0
17	39.337883891331465	41.0	40.0	41.0	37.0	41.0
18	39.31159821037799	41.0	40.0	41.0	37.0	41.0
19	39.29885528211608	41.0	40.0	41.0	37.0	41.0
20	39.26440986528145	41.0	40.0	41.0	37.0	41.0
21	39.24056427356062	41.0	40.0	41.0	36.0	41.0
22	39.20643647484575	41.0	39.0	41.0	36.0	41.0
23	39.16230195901714	41.0	39.0	41.0	36.0	41.0
24	39.121921933491755	41.0	39.0	41.0	36.0	41.0
25	39.08894095907277	41.0	39.0	41.0	36.0	41.0
26	39.026656553488735	41.0	39.0	41.0	36.0	41.0
27	38.9571506115166	41.0	39.0	41.0	36.0	41.0
28	38.91728872732929	41.0	39.0	41.0	36.0	41.0
29	38.82674196219297	40.0	39.0	41.0	35.0	41.0
30	38.7893783546186	40.0	39.0	41.0	35.0	41.0
31	38.751288304966685	40.0	39.0	41.0	35.0	41.0
32	38.67836165797306	40.0	39.0	41.0	35.0	41.0
33	38.61831382981884	40.0	38.0	41.0	35.0	41.0
34	38.57201075386324	40.0	38.0	41.0	35.0	41.0
35	38.519308362329504	40.0	38.0	41.0	35.0	41.0
36	38.434148106686706	40.0	38.0	41.0	34.0	41.0
37	38.37143914206741	40.0	38.0	41.0	34.0	41.0
38	38.31229505466679	40.0	38.0	41.0	34.0	41.0
39	38.24475981412445	40.0	38.0	41.0	34.0	41.0
40	38.16738258868669	40.0	38.0	41.0	34.0	41.0
41	38.326008768795	40.0	38.0	41.0	34.0	41.0
42	38.36020282046763	40.0	38.0	41.0	34.0	41.0
43	38.34482936172657	40.0	38.0	41.0	34.0	41.0
44	38.30790377982126	40.0	38.0	41.0	34.0	41.0
45	38.266380710739405	40.0	38.0	41.0	34.0	41.0
46	38.16675415777818	40.0	38.0	41.0	34.0	41.0
47	38.129746803879904	40.0	38.0	41.0	34.0	41.0
48	38.053048527230956	40.0	38.0	41.0	33.0	41.0
49	37.99549785947136	40.0	38.0	41.0	33.0	41.0
50	37.91034098849053	40.0	38.0	41.0	33.0	41.0
51	36.38340752284332	38.0	35.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	2.0
5	20.0
6	402.0
7	2413.0
8	8587.0
9	13438.0
10	13728.0
11	15240.0
12	18871.0
13	23842.0
14	28467.0
15	31983.0
16	33764.0
17	34837.0
18	35774.0
19	36837.0
20	38913.0
21	41661.0
22	45553.0
23	51450.0
24	57317.0
25	65900.0
26	76707.0
27	90403.0
28	108098.0
29	130739.0
30	162054.0
31	203333.0
32	265867.0
33	362118.0
34	530154.0
35	810328.0
36	1270372.0
37	2146024.0
38	5034653.0
39	1.5978139E7
40	4356.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.490887739909816	27.785548984148072	29.118784086648237	23.604779189293875
2	22.349762370027264	28.956354508848246	29.913575352592183	18.78030776853231
3	22.01882701303535	27.528264429431793	29.958934063035947	20.49397449449691
4	21.631826072990776	26.108064837317933	30.68467519223214	21.57543389745915
5	25.09577908684656	26.35601857318609	28.53270223767647	20.015500102290883
6	21.8561344916889	26.12150102094739	31.286913653790453	20.73545083357326
7	24.278790232332035	26.671641328200053	28.87025659881901	20.179311840648896
8	22.095974963741817	26.56424039451667	30.770351502346898	20.569433139394615
9	22.154644239915513	26.520344349772852	30.773216157937583	20.551795252374056
10	22.163132148779226	27.489740325911416	29.81624587929647	20.530881646012887
11	24.476034986749887	26.512335799734593	28.2817256492094	20.729903564306117
12	21.6900349040699	29.18818857424443	28.305231101575817	20.816545420109854
13	23.299483159775434	26.852701634363747	28.754814395555435	21.093000810305387
14	23.53160324373974	27.097146621855828	28.15916667740942	21.21208345699501
15	22.943372754103223	27.779525805681025	27.882147326608376	21.39495411360738
16	23.16654094074025	27.211663638267886	28.091011351649662	21.5307840693422
17	23.031001154605722	27.2484069483751	28.205089519301975	21.51550237771721
18	22.91288131835029	27.202329618322384	28.22514377107128	21.659645292256048
19	22.695642570663622	27.42342778074866	28.38120098891676	21.499728659670954
20	22.50788822095286	27.465692882287595	28.387239597248836	21.63917929951071
21	22.581737131211145	27.45802283983847	28.279605280924976	21.680634748025415
22	22.452577491314234	27.543951299949665	28.397713812955	21.6057573957811
23	22.50777357966628	27.611078760703663	28.25360442190622	21.627543237723838
24	22.393567571020007	27.629103973790276	28.208603572972063	21.76872488221766
25	22.374523801250273	27.774235475836857	28.150052366333515	21.70118835657935
26	22.417507712321008	27.73054839674543	28.11431527632663	21.73762861460693
27	22.4411297359099	27.68795927908409	28.00785149376387	21.863059491242137
28	22.40220435271444	27.751522423851803	28.020325989991836	21.825947233441926
29	22.46665512365036	27.736300369083384	27.96358774247002	21.833456764796242
30	22.494222679313786	27.690850415553577	27.900687572842365	21.91423933229028
31	22.548831418028048	27.67454014939687	27.846156975997875	21.9304714565772
32	22.602590079566358	27.727755531463632	27.758001875856742	21.91165251311326
33	22.549458337241987	27.648155346938026	27.742848875847443	22.059537439972544
34	22.573750095350437	27.679721794981045	27.738876880996678	22.007651228671847
35	22.597323472826616	27.66791028583938	27.684598471766947	22.05016776956705
36	22.61797356207607	27.665170044970672	27.673920604124202	22.04293578882906
37	22.57553286775774	27.638093935434743	27.707369543992115	22.0790036528154
38	22.583745906185218	27.656372682495352	27.764020745957847	21.995860665361583
39	22.560175816748266	27.557749743554353	27.74309863837745	22.13897580131993
40	22.61679063948884	27.563474089754603	27.708361577737072	22.11137369301948
41	22.597698703737223	27.607559889388767	27.694830716132905	22.099910690741105
42	22.559012885196918	27.557939304860152	27.706684742864002	22.176363067078928
43	22.598213355423738	27.633003688012597	27.618694771065073	22.150088185498596
44	22.67152739206689	27.572593989900966	27.621877758393005	22.134000859639137
45	22.643768314162543	27.527456163947967	27.62396372044751	22.204811801441977
46	22.56263804596253	27.65716213843853	27.55473591525072	22.22546390034822
47	22.602232172858237	27.597120681261387	27.60005583637407	22.200591309506308
48	22.58686956156158	27.51505930722954	27.553873226764253	22.344197904444627
49	22.613524295873585	27.61985965382554	27.532633476163216	22.23398257413766
50	22.566533197967203	27.612172431856386	27.580173088992183	22.241121281184228
51	22.599545086098917	27.57071326382789	27.563106731366656	22.266634918706536
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.09566692920369
2	41.130070138559574
3	42.51280150753226
4	43.207259970449925
5	45.111279189137434
6	42.59158532526216
7	44.458102072980935
8	42.66540810313643
9	42.70643949228957
10	42.69401379479211
11	45.205938551056
12	42.50658032417975
13	44.39248397008082
14	44.743686700734756
15	44.3383268677106
16	44.69732501008245
17	44.54650353232293
18	44.57252661060633
19	44.19537123033458
20	44.14706752046357
21	44.26237187923656
22	44.058334887095334
23	44.13531681739012
24	44.16229245323767
25	44.07571215782963
26	44.15513632692794
27	44.30418922715204
28	44.22815158615636
29	44.300111888446594
30	44.40846201160406
31	44.479302874605246
32	44.51424259267962
33	44.60899577721453
34	44.58140132402228
35	44.64749124239367
36	44.66090935090513
37	44.65453652057314
38	44.579606571546805
39	44.69915161806819
40	44.72816433250832
41	44.69760939447833
42	44.73537595227584
43	44.74830154092233
44	44.80552825170603
45	44.84858011560452
46	44.788101946310746
47	44.802823482364545
48	44.931067466006205
49	44.847506870011244
50	44.80765447915143
51	44.86618000480546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9649.0
1	6573.5
2	3498.0
3	4917.5
4	6337.0
5	12822.5
6	19308.0
7	24655.0
8	30002.0
9	24702.0
10	19402.0
11	21758.5
12	24115.0
13	31771.5
14	39428.0
15	54596.0
16	69764.0
17	101516.5
18	133269.0
19	169143.0
20	205017.0
21	245754.5
22	286492.0
23	340042.5
24	393593.0
25	468601.0
26	638419.5
27	733230.0
28	802396.0
29	871562.0
30	933285.5
31	995009.0
32	1075986.0
33	1156963.0
34	1235857.5
35	1314752.0
36	1440744.0
37	1566736.0
38	1641153.5
39	1715571.0
40	1793880.5
41	1872190.0
42	1885719.5
43	1899249.0
44	1907267.0
45	1915285.0
46	1887050.5
47	1858816.0
48	1815208.5
49	1771601.0
50	1691607.0
51	1611613.0
52	1502498.0
53	1393383.0
54	1287107.5
55	1180832.0
56	1076981.0
57	973130.0
58	863043.5
59	752957.0
60	671016.0
61	589075.0
62	510858.5
63	432642.0
64	374022.0
65	315402.0
66	265709.0
67	216016.0
68	204614.0
69	193212.0
70	174145.5
71	155079.0
72	133491.5
73	111904.0
74	97101.5
75	69861.5
76	57424.0
77	54372.0
78	51320.0
79	50936.5
80	50553.0
81	44801.5
82	39050.0
83	35886.0
84	32722.0
85	29389.0
86	26056.0
87	30088.0
88	34120.0
89	22860.0
90	11600.0
91	7619.5
92	3639.0
93	2845.0
94	2051.0
95	1569.0
96	1087.0
97	812.0
98	537.0
99	366.0
100	195.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0036871211271500477
2	6.301232395031821E-4
3	0.005030183803348258
4	0.009127785212231808
5	0.012588061973160711
6	3.6367112679897934E-4
7	0.027516581693047527
8	0.007421051409234618
9	0.012973337325314084
10	0.003582700704603806
11	0.018122344368111516
12	0.018349188734332663
13	0.013689877466234847
14	0.006059985211907745
15	0.0049221626765762845
16	0.05834221056954319
17	0.0034566760567031696
18	8.713704226272575E-4
19	0.00341346760599438
20	0.002005592253732985
21	0.002502489436884066
22	0.003741131690536035
23	2.4844859157554036E-4
24	0.04636986901898273
25	0.005905154930201249
26	0.01131701338147715
27	0.03778579014483653
28	7.201408451464938E-5
29	0.09668971057359399
30	0.03723488239829946
31	3.600704225732469E-5
32	0.013322605635210132
33	0.009937943663021614
34	0.02285366972072398
35	0.015115756339624906
36	0.009689495071446074
37	0.017989118311759415
38	0.054827923245228304
39	0.018910898593546926
40	0.03144495000332165
41	0.021269359861401695
42	0.019598633100661827
43	0.02319573662216856
44	0.009192597888294993
45	0.012728489437964277
46	0.032215500707628394
47	0.019800272537302845
48	0.05135324366739647
49	0.007244616902173727
50	0.05590093310449658
51	0.11893126057594344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.7772345E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.983961059324532
#Duplication Level	Relative count
1	100.0
2	9.582646042762441
3	2.374031309327441
4	1.1895054620148766
5	0.6990134138371044
6	0.5016961812579752
7	0.3821854284024774
8	0.31060346705673647
9	0.23902150571099562
10++	9.211664747440167
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	8285185	3.4813685	4.2439685	16
TGTGT	4973525	3.2862427	25.161476	4
AAAAA	6294970	3.0875256	3.3016446	10
TGTTT	4727645	2.491073	20.286818	6
GTTTG	3529050	2.33181	24.549187	7
TTTGA	4001505	2.174697	20.456282	8
CTTTG	3129930	2.161163	25.83627	1
GTGTT	3214860	2.1242096	24.479673	5
GAGGG	1911480	2.0484548	7.4131017	11
TTGTG	3009045	1.9882181	24.485538	3
TTTGT	3709045	1.9543557	19.948524	2
TGAGG	2041005	1.7442427	9.265737	10
TTGAG	2545265	1.734611	15.281871	9
ACTTT	2482630	1.4099541	7.7070327	3
AACTT	2378590	1.3933058	8.212939	2
TAACT	1716080	1.0052274	7.9528813	1
>>END_MODULE